Open Access
Research
(Published
online: 29-11-2016)
25.
Steroid and metabolic hormonal
profile of porcine serum vis-à-vis ovarian follicular fluid
-
Soumen Naskar, S. Borah, Y. Vashi, R. Thomas, D. K. Sarma, J.
Goswami
and S. K. Dhara
Veterinary World, 9(11): 1320-1323
doi:
10.14202/vetworld.2016.1320-1323
Soumen Naskar :
ICAR-National
Research Centre on Pig, Guwahati - 781 131, Assam, India;
ICAR-Indian
Institute of Agricultural Biotechnology, Ranchi - 834 010,
Jharkhand, India; snrana@gmail.com
S. Borah :
Department of
Physiology and Biochemistry, Lakhimpur College of Veterinary
Science (AAU), Lakhimpur - 787 051, Assam, India;
sbsborah06@gmail.com
Y. Vashi :
ICAR-National
Research Centre on Pig, Guwahati - 781 131, Assam, India;
yoyavashi@gmail.com
R. Thomas :
ICAR-National
Research Centre on Pig, Guwahati - 781 131, Assam, India;
thomasrlpt@gmail.com
D. K. Sarma :
ICAR-National
Research Centre on Pig, Guwahati - 781 131, Assam, India;
dksarma1956@gmail.com
J. Goswami :
Department of
Veterinary Physiology, College of Veterinary Science (AAU),
Guwahati - 781 022, Assam, India;
jeetendragoswami@yahoo.co.in
S. K. Dhara :
Division of
Veterinary Biotechnology, ICAR-Indian Veterinary Research
Institute, Izatnagar - 243 122, Uttar Pradesh, India;
sujoylab.office@gmail.com
Received: 09-05-2016, Accepted: 20-10-2016, Published online:
29-11-2016
Corresponding author:
Soumen Naskar,
e-mail: snrana@gmail.com
Citation:
Naskar S, Borah S, Vashi Y, Thomas R, Sarma DK, Goswami J, Dhara
SK (2016) Steroid and metabolic hormonal profile of porcine serum
vis-à-vis ovarian follicular fluid, Veterinary World, 9(11):
1320-1323.
Abstract
Aim:
This study was
conducted to understand whether serum level of the steroid and
metabolic hormones may be indicative of their level in ovarian
follicular fluid (FF) in porcine, and its influence on fertility.
Materials and
Methods:
Ovaries from pigs
(n=32) of two genetic groups, namely, native (Ghungroo; n=16) and
crossbred (Hampshire × Ghungroo; n=16) were collected. Both the
genetic groups comprised gilts (n=8) and sows (n=8), and sows were
in luteal phase of estrus cycle. FF was aspirated from small,
medium and large follicles, and centrifuged for the collection of
supernatant for further analysis. Blood samples were collected
from the same animals, and serum was separated. Hormones, namely,
cortisol, T3, T4 and testosterone were estimated by
radioimmunoassay. Two-way ANOVA was used for analysis of data
considering genetic background (native or crossbred), stage of
reproductive life (gilt or sow), and source of sample (serum or
FF) as fixed effects.
Results:
It was
observed that all the hormones except cortisol differed
significantly (p<0.01) based on genetic background. Stage of
reproductive life and source of sample did not affect the studied
hormonal level. Within the genetic groups, stage of reproductive
life influenced T3 (p<0.01), cortisol (p<0.05) and testosterone
(p<0.01) level in crossbred pigs as compared to T3 (p<0.01) only
in native pigs. The level of T3 in serum, as well as FF, was
higher (p<0.01) in Ghungroo gilts compared to sows. However, a
reverse of this was observed in the case of crossbred pigs. The
level of cortisol (p<0.05) and testosterone (p<0.01) was higher in
crossbred sows than gilts in both serum and FF.
Conclusion:
The
study revealed that serum level of the steroid and metabolic
hormones is indicative of their level in the ovarian FF. Further,
varying level of steroid and metabolic hormones in pigs based on
genetic background may be due to variation in body size, rate of
energy metabolism and stage of (re)productive life.
Keywords:
cortisol, follicular fluid, pig, serum, T3, T4, testosterone.
References
1. Tabatabaei, S. and Mamoei, M. (2011) Biochemical
composition of blood plasma and follicular fluid in relation
to follicular size in buffalo. Comp. Clin. Pathol., 20:
441-445.
https://doi.org/10.1007/s00580-010-1014-5 |
|
|
2. Sato, E. and Jiang, J.Y. (2001) Follicular development and
ovulation in hypothyroid rdw rats. Ital. J. Anat. Embryol.,
106 2 Suppl 2: 249-256.
PMid:11732584 |
|
|
|
3. Kor, N.M. and Moradi, K. (2013) A review of biochemical
metabolites concentration and hormonal composition of ovarian
follicular fluid in domestic animals. Annu. Rev. Res. Biol.,
3(3): 246-255. |
|
|
|
4. Snedecor, G.W. and Cochran, W.G. (1994) Statistical
Methods. 8th ed. The IOWA State University Press, IOWA. |
|
|
|
5. Ali, S., Ahmad, N., Akhtar, N., Rahman, Z.U. and Ahmad, M.
(2011) Hormonal profiles in the serum and follicular fluid of
female camel (Camelus dromedarius) during the peak and the low
breeding season. Pak. Vet. J., 31(4): 331-335. |
|
|
6. Gregoraszczuk, E.L., Slomczynska, M. and Wilk, R. (1998)
Thyroid hormone inhibits aromatase activity in porcine thecal
cells cultured alone and in coculture with granulosa cells.
Thyroid, 8: 1157-1163.
https://doi.org/10.1089/thy.1998.8.1157
PMid:9920372 |
|
|
7. Maruo, T., Hayashi, M., Matsuo, H., Yamamoto, T., Okada, H.
and Mochizuki, M. (1987) The role of thyroid hormones as a
biological amplifier of the actions of follicle-stimulating
hormone in the functional differentiation of cultured porcine
granulosa cells. Endocrinology, 121: 1233-1241.
https://doi.org/10.1210/endo-121-4-1233
PMid:3115761 |
|
|
|
8. Huszenicza, G.Y., Kulcsar, M. and Rudas, P. (2002) Clinical
endocrinology of thyroid gland function in ruminants. Vet.
Med., 47(7): 199-210. |
|
|
9. Sheikh, N., Amiri, I., Farimani, M., Yavangi, M. and Rabiee,
S. (2011) Interleukin 6, interleukin 1β, estradiol and
testosterone concentrations in serum and follicular fluid of
females with stimulated and non-stimulated ovaries. Afr. J.
Biotechnol., 10(46): 9410-9414.
https://doi.org/10.5897/AJB11.680 |
|
|
10. Costa, L.O., Mendes, M.C., Ferriani, R.A., Moura, M.D.,
Reis, E.M. and Silva, M.F. (2004) Estradiol and testosterone
concentrations in follicular fluids as criteria to
discriminate between mature and immature oocytes. Braz. J.
Med. Biol. Res., 73(11): 1747-1755.
https://doi.org/10.1590/S0100-879X2004001100021 |
|
|
11. Spicer, L.J., Alonso, J. and Chamberlain, C.S. (2001)
Effects of thyroid hormone on bovine granulose and thecal cell
function in-vitro: Dependence on insulin and gonadotropins. J.
Dairy Sci., 84: 1069-1076.
https://doi.org/10.3168/jds.S0022-0302(01)74567-5 |
|
|
12. Kim, M.K., Fibrianto, Y.X., Oh, H.J., Jang, G., Kim, H.J.,
Lee, H.K., Kang, S.K., Lee, C.B. and Hwang, W.S. (2005)
Effects of estradiol-l7 and progesterone supplementation on
in vitro nuclear maturation of canine oocytes. Theriogenology,
63: 1342-1353.
https://doi.org/10.1016/j.theriogenology.2004.07.019
PMid:15725442 |
|
|
13. Zhang, S.S., Carrillo, A.J. and Darling, D.S. (1997)
Expression of multiple thyroid hormone receptor mRNAs in human
oocytes, cumulus cells, and granulosa cells. Mol. Hum. Reprod.,
3: 555-562.
https://doi.org/10.1093/molehr/3.7.555
PMid:9268132 |
|
|
14. Lewicka, S., Hagens, C.V., Von, C., Hettinger, U.,
Grunwald, K., Vecsei, P., Runnebaum, B. and Rabe, T. (2003)
Cortisol and cortisone in human follicular fluid and serum and
the outcome of IVF treatment. Hum. Reprod., 18(8): 1613-1617.
https://doi.org/10.1093/humrep/deg352 |
|
|
15. Paredes, A.H., Salvetti, N.R., Diaz, A.E., Dallard, B.E.,
Ortega, H.H. and Lara, H.E. (2011) Sympathetic nerve activity
in normal and cystic follicles from isolated bovine ovary:
Local effect of beta-adrenergic stimulation on steroid
secretion. Reprod. Biol. Endocrinol., 9: 66.
https://doi.org/10.1186/1477-7827-9-66 |
|
|
16. Spicer, L.J. and Zinn S.A. (1987) Relationship between
concentrations of cortisol in ovarian follicular fluid and
various biochemical markers of follicular differentiation in
cyclic and anovulatory cattle. J. Reprod. Fertil., 81:
221-226.
https://doi.org/10.1530/jrf.0.0810221
PMid:3668952 |
|
