Open Access
Research
(Published
online: 21-09-2016)
13.
Occurrence and characteristics of extended-spectrum β-lactamases
producing
Escherichia coli
in foods of animal origin and human
clinical samples in Chhattisgarh, India -
Bhoomika, Sanjay Shakya, Anil Patyal and Nitin Eknath Gade
Veterinary World, 9(9): 996-1000
doi:
10.14202/vetworld.2016.996-1000
Bhoomika:
Department of Veterinary Public Health and Epidemiology, College
of Veterinary Science and Animal Husbandry, Chhattisgarh
Kamdhenu Vishwa Vidyalaya, Anjora, Durg, Chhattisgarh, India;
sirsant.mannat.09@gmail.com
Sanjay Shakya:
Department of Veterinary Public Health and Epidemiology, College
of Veterinary Science and Animal Husbandry, Chhattisgarh
Kamdhenu Vishwa Vidyalaya, Anjora, Durg, Chhattisgarh, India;
shakyadurg@gmail.com
Anil Patyal:
Department of Veterinary Public Health and Epidemiology, College
of Veterinary Science and Animal Husbandry, Chhattisgarh
Kamdhenu Vishwa Vidyalaya, Anjora, Durg, Chhattisgarh, India;
anilvet01@gmail.com
Nitin Eknath Gade:
Department of Veterinary Physiology and Biochemistry, College of
Veterinary Science and Animal Husbandry, Chhattisgarh Kamdhenu
Vishwa Vidyalaya, Anjora, Durg, Chhattisgarh, India;
nitingadeivri@gmail.com
Received: 29-03-2016, Accepted: 10-08-2016, Published online:
21-09-2016
Corresponding author:
Sanjay Shakya, e-mail: shakyadurg@gmail.com
Citation:
Bhoomika, Shakya S, Patyal A, Gade NE (2016) Occurrence and
characteristics of extended-spectrum β-lactamases producing
Escherichia coli
in foods of animal origin and human clinical samples in
Chhattisgarh, India,
Veterinary World, 9(9):
996-1000.
Abstract
Aim:
To assess the prevalence of antimicrobial resistance producing
extended-spectrum β-lactamases (ESBL) (blaTEM,
blaSHV,
and
blaCTX-M)
genes in
Escherichia coli
isolated from chicken meat, chevon meat, raw milk, and human
urine and stool samples collected from tribal districts of
Chhattisgarh,
viz.,
Jagdalpur, Dantewada, Kondagaon, and Kanker.
Materials and Methods:
A total of 330 samples, comprising 98 chicken meat, 82 chevon
meat, 90 raw milk, and 60 human urine and stool samples, were
processed for isolation of
E. coli.
Isolates were confirmed biochemically and further tested against
commonly used antibiotics to know their resistant pattern. The
resistant isolates were tested for ESBL production by phenotypic
method followed by characterization with molecular method using
multiplex-polymerase chain reaction technique.
Results:
Overall 57.87% (191/330) samples were found positive for
E. coli,
which include 66.32% (65/98) chicken meat, 46.34% (38/82) chevon
meat, 81.11% (73/90) raw milk, and 25% (15/60) human urine and
stool samples. Isolates showed the highest resistance against
cefotaxime (41.36%) followed by oxytetracycline (34.03%),
ampicillin (29.31%), cephalexin (24.60%), cefixime (16.75%), and
ceftazidime (13.08%). Phenotypic method detected 10.99% (21/191)
isolates as presumptive ESBL producers, however, molecular
method detected 3.66% (7/191), 2.09% (4/191), and 0.00% (0/191)
prevalence of
blaTEM,
blaCTX-M,
and
blaSHV,
respectively.
Conclusion:
The present study indicates a high prevalence of
E. coli
in raw chicken meat, chevon meat, and milk due to poor hygienic
practices. The antibiotic susceptibility test detected the
presence of the resistance pattern against ESBL in
E. coli
isolated from raw chicken meat, chevon meat, milk, and also in
human clinical samples is of great concern. The appearance of
E. coli
in the human food chain is alarming and requires adaptation of
hygienic practices and stipulate use of antibiotics.
Keywords:
blaTEM,
blaSHV,
blaCTX-M,
Escherichia coli,
extended-spectrum β-lactamases.
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