Open Access
Research
(Published
online: 23-09-2016)
14.
The expression of cyclic adenosine
monophosphate responsive element modulator in rat sertoli cells
following seminal extract administration -
Muslim Akmal, Tongku Nizwan Siregar, Sri Wahyuni, Hamny, Mustafa
Kamal Nasution, Wiwik Indriati, Budianto Panjaitan and Dwinna
Aliza
Veterinary World, 9(9): 1001-1005
doi:
10.14202/vetworld.2016.1001-1005
Muslim Akmal:
Laboratory of Histology, Faculty of Veterinary Medicine, Syiah
Kuala University, Banda Aceh, Aceh, Indonesia; muslim_akmal70@yahoo.com
Tongku Nizwan Siregar:
Laboratory of Reproduction, Faculty of Veterinary Medicine,
Syiah Kuala University, Banda Aceh, Aceh, Indonesia; tongku_ns@yahoo.com
Sri Wahyuni:
Laboratory of Research, Faculty of Veterinary Medicine, Syiah
Kuala University, Banda Aceh, Aceh, Indonesia; Laboratory of
Anatomy, Faculty of Veterinary Medicine, Syiah Kuala University,
Banda Aceh, Aceh, Indonesia; yuyun.anwar@gmail.com
Hamny:
Laboratory of Anatomy, Faculty of Veterinary Medicine, Syiah
Kuala University, Banda Aceh, Aceh, Indonesia; hamny@unsyiah.ac.id
Mustafa Kamal Nasution:
Department of PGMI , Faculty of Tarbiyah, STAIN Gajah Putih
Takengon, Aceh Tengah, Aceh, Indonesia; kamalnasution@gmail.com
Wiwik Indriati:
Student at Veterinary Public Health Graduate Program, Syiah
Kuala University, Banda Aceh, Aceh, Indonesia; indria@yahoo.co.id
Budianto Panjaitan:
Laboratory of Clinic, Faculty of Veterinary Medicine, Syiah
Kuala University, Banda Aceh, Aceh, Indonesia; antopjt@gmail.com
Dwinna Aliza:
Laboratory of Pathology, Faculty of Veterinary Medicine, Syiah
Kuala University, Banda Aceh, Aceh, Indonesia; dwinna2000@yahoo.com
Received: 30-03-2016, Accepted: 10-08-2016, Published online:
23-09-2016
Corresponding author:
Budianto Panjaitan, e-mail: antopjt@gmail.com
Citation:
Akmal M, Siregar TN, Wahyuni S, Hamny, Nasution MK, Indriati W,
Panjaitan B, Aliza D (2016) The expression of cyclic adenosine
monophosphate responsive element modulator in rat sertoli cells
following seminal extract administration,
Veterinary World, 9(9):
1001-1005.
Abstract
Aim:
This study aims to determine the effect of seminal vesicle
extract on cyclic adenosine monophosphate responsive element
modulator (CREM) expression in rat Sertoli cells.
Materials and Methods:
This study examined the expression of CREM on 20 male rats (Rattus
norvegicus)
at 4 months of age, weighing 250-300 g. The rats were divided
into four groups: K0, KP1, KP2, and KP3. K0 group was injected
with 0.2 ml normal saline; KP1 was injected with 25 mg
cloprostenol (Prostavet C, Virbac S. A); KP2 and KP3 were
injected with 0.2 and 0.4 ml seminal vesicle extract,
respectively. The treatments were conducted 5 times within
12-day interval. At the end of the study, the rats were
euthanized by cervical dislocation; then, the testicles were
necropsied and processed for histology observation using
immunohistochemistry staining.
Results:
CREM expression in rat Sertoli cells was not altered by the
administration of either 0.2 or 0.4 ml seminal vesicle extract.
Conclusion:
The administration of seminal vesicle extract is unable to
increase CREM expression in rat Sertoli cells.
Keywords:
cyclic adenosine monophosphate responsive element modulator,
seminal vesicle extract, Sertoli cells, spermatogenesis.
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