Open Access
Research
(Published
online: 24-09-2016)
15.
Enzyme histochemistry of cecal lymphoid
tissue during prenatal period of buffalo -
Kritima Kapoor and Opinder Singh
Veterinary World, 9(9): 1006-1011
doi:
10.14202/vetworld.2016.1006-1011
Kritima Kapoor:
Department of Veterinary Anatomy, Guru Angad Dev Veterinary and
Animal Sciences University, Ludhiana, Punjab, India;
kritimakapoor89@gmail.com
Opinder Singh:
Department of Veterinary Anatomy, Guru Angad Dev Veterinary and
Animal Sciences University, Ludhiana, Punjab, India;
singhopinder68@gmail.com
Received: 22-04-2016, Accepted: 11-08-2016, Published online:
24-09-2016
Corresponding author:
Kritima Kapoor, e-mail: kritimakapoor89@gmail.com
Citation:
Kapoor K, Singh O (2016) Enzyme histochemistry of cecal lymphoid
tissue during prenatal period of buffalo,
Veterinary World, 9(9):
1006-1011.
Abstract
Aim:
This study was designed to elucidate the histoenzymic
distribution of enzymes, i.e., phosphatases, oxidoreductases,
dehydrogenases, and diaphorases in cecal lymphoid tissue during
its development in the prenatal period.
Materials and Methods:
The study was conducted on cecum of 15 buffalo fetuses ranging
from 16 cm curved crownrump length (CVRL) (100 days) to 100 cm
CVRL (full term). The fetuses were categorized into three groups
based on their CVRL.
Results:
In Group I, the distribution of enzymes was uniformly weak in
developing villi-like projections in cecum and completely absent
from submucosa. In Group II, the enzymes showed a moderate to
strong activity in epithelium lining tunica mucosa which
progressively decreased as the fetus progresses toward late
gestational age. However, the intense activity of enzymes was
observed in developing lymphoid tissue in this group. In Group
III, distribution of enzymes reduced in tunica mucosa of cecum
with advancing age, whereas the intense activity was noticed in
the developed lymphoid tissue complex.
Conclusion:
The distribution of enzymes was completely absent from
submucosal region in cecum of Group I as there was no lymphoid
tissue development at this age. In Group II, the enzymes showed
a moderate to strong activity in epithelium lining tunica mucosa
which progressively decreased toward late gestational age but an
intense activity was observed in developing lymphoid tissue. In
Group III, distribution of enzymes reduced in tunica mucosa with
advancing age with intense activity noticed in the developed
lymphoid tissue complex.
Keywords:
buffalo, cecum, histoenzyme, lymphoid tissue, prenatal.
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