Open Access
Research
(Published
online: 28-09-2016)
18.
Detection of
Peste des petits ruminants
virus and goatpox virus from an outbreak
in goats with high mortality in Meghalaya state, India -
A. Karim, U. Bhattacharjee, K. Puro, I. Shakuntala, R. Sanjukta,
S. Das, S. Ghatak and A. Sen
Veterinary World, 9(9): 1025-1027
doi:
10.14202/vetworld.2016.1025-1027
A. Karim:
Division of Animal Health, Indian Council of Agricultural
Research - North Eastern Hill Region, Umiam - 793 013, Meghalaya,
India; kihom33@gmail.com
U. Bhattacharjee:
Division of Animal Health, Indian Council of Agricultural
Research - North Eastern Hill Region, Umiam - 793 013, Meghalaya,
India; uttaranbhattacharjee@gmail.com
K. Puro:
Division of Animal Health, Indian Council of Agricultural
Research - North Eastern Hill Region, Umiam - 793 013, Meghalaya,
India; akulepuro@rediffmail.com
I. Shakuntala:
Division of Animal Health, Indian Council of Agricultural
Research - North Eastern Hill Region, Umiam - 793 013, Meghalaya,
India; drshakuntala@rediffmail.com
R. Sanjukta:
Division of Animal Health, Indian Council of Agricultural
Research - North Eastern Hill Region, Umiam - 793 013, Meghalaya,
India; rajkumari.sanjukta@gmail.com
S. Das:
Division of Animal Health, Indian Council of Agricultural
Research - North Eastern Hill Region, Umiam - 793 013, Meghalaya,
India; drsamirvph@gmail.com
S. Ghatak:
Division of Animal Health, Indian Council of Agricultural
Research - North Eastern Hill Region, Umiam - 793 013, Meghalaya,
India; ghataksnd@rediffmail.com
A. Sen:
Division of Animal Health, Indian Council of Agricultural
Research - North Eastern Hill Region, Umiam - 793 013, Meghalaya,
India; arnabsen123@gmail.com
Received: 19-05-2016, Accepted: 09-08-2016, Published online:
28-09-2016
Corresponding author:
K. Puro, e-mail: akulepuro@rediffmail.com
Citation:
Karim A, Bhattacharjee U, Puro K, Shakuntala I, Sanjukta R, Das
S, Ghatak S, Sen A (2016) Detection of
Peste des petits ruminants
virus and goatpox virus from an outbreak in goats with high
mortality in Meghalaya state, India,
Veterinary World, 9(9):
1025-1027.
Abstract
Aim:
We describe a laboratory investigation carried out to confirm
the etiology of the heavy mortality (37 animals died out of
total 44, i.e. 84%) in goats in Ri-Bhoi district of Meghalaya,
Northeast region of India in December 2015. The clinical signs
observed were abortion, diarrhea, high fever (up to 104°F), pox
lesion in the skin, and respiratory distress.
Materials and Methods:
The samples comprising whole blood, sera, and pox lesion were
collected from the animals (n=7) from an outbreak for the
screening of
peste des petits ruminants
(PPR) and poxviruses. The whole blood and sera were used for
screening of PPR virus (PPRV) by sandwich enzyme-linked
immunosorbent assay (ELISA) and antibody by competitive ELISA as
well as detection of PPRV partial N gene by reverse
transcription-polymerase chain reaction (PCR). The skin lesions
were used for the detection of poxvirus by PCR.
Results:
The results showed the presence of PPR antigens (58-80%) in the
samples by sandwich ELISA and antibody in all the sera samples
ranging from 9% to 41% positivity in competitive ELISA. Four
samples were positive for PPRV partial N gene. The skin lesion
screened for poxvirus was also found to be positive for I3L gene
of goatpox virus.
Conclusion:
We confirm the outbreak of disease in goats with high mortality
is a case of mixed infection of PPR and goatpox detected for the
first time in Northeast India.
Keywords:
Capripoxvirus,
disease, goat, mortality,
Peste des petits ruminants.
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