Development and validation of a regionally adapted sandwich enzyme-linked immunosorbent assay targeting recombinant p60 antigen for rapid detection of Listeria monocytogenes in food samples

Veterinary World Volume 18, Issue 12

Dec 2025

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ARTICLE

Development and validation of a regionally adapted sandwich enzyme-linked immunosorbent assay targeting recombinant p60 antigen for rapid detection of Listeria monocytogenes in food samples

Guldarigash Kaukabayeva , Aigerim Turgimbayeva , Zhanar Akhmetkarimova , Sholpan Mukhlis , Gulkhan Unysheva , Sailau Abeldenov , Pavel Shevchenko , Albina Gabitova , Raushan Rychshanova , Aralbek Rsaliyev , Yergali Abduraimov , Saule Eskendirova
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¹ Stem Cell Laboratory, National Center for Biotechnology, 13/5 Korgalzhyn Highway, Astana, 010000, Kazakhstan.

² Stem Cell Laboratory, National Center for Biotechnology, 13/5 Korgalzhyn Highway, Astana, 010000, Kazakhstan.

³ Stem Cell Laboratory, National Center for Biotechnology, 13/5 Korgalzhyn Highway, Astana, 010000, Kazakhstan.

⁴ Stem Cell Laboratory, National Center for Biotechnology, 13/5 Korgalzhyn Highway, Astana, 010000, Kazakhstan.

⁵ Stem Cell Laboratory, National Center for Biotechnology, 13/5 Korgalzhyn Highway, Astana, 010000, Kazakhstan.

⁶ Stem Cell Laboratory, National Center for Biotechnology, 13/5 Korgalzhyn Highway, Astana, 010000, Kazakhstan.

⁷ Research Institute of Applied Biotechnology, Baitursynov Kostanay University, Mayakovskiy Street 99/1, Kostanay, 110000, Kazakhstan.

⁸ Research Institute of Applied Biotechnology, Baitursynov Kostanay University, Mayakovskiy Street 99/1, Kostanay, 110000, Kazakhstan.

⁹ Research Institute of Applied Biotechnology, Baitursynov Kostanay University, Mayakovskiy Street 99/1, Kostanay, 110000, Kazakhstan.

¹⁰ Stem Cell Laboratory, National Center for Biotechnology, 13/5 Korgalzhyn Highway, Astana, 010000, Kazakhstan

¹¹ Stem Cell Laboratory, National Center for Biotechnology, 13/5 Korgalzhyn Highway, Astana, 010000, Kazakhstan

¹² National Center for Biotechnology, 13/5 Korgalzhyn Highway, Astana, 010000, Kazakhstan.

Corresponding Author: Saule Eskendirova (eskendirova@biocenter.kz)

Kaukabayeva et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License.

https://doi.org/10.14202/vetworld.2025.3839-3854

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Veterinary World

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Development and validation of a regionally adapted sandwich enzyme-linked immunosorbent assay targeting recombinant p60 antigen for rapid detection of Listeria monocytogenes in food samples

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