Open Access
Research (Published online: 15-07-2017)
12. Semen characteristics, extension, and cryopreservation of Rusa deer (Rusa timorensis)
Wan-Nor Fitri, Haron Wahid, Yusoff Rosnina, Faez Firdaus Abdullah Jesse, Zainal Abidin Aimi-Sarah, Mohd Lila Mohd-Azmi, Che' Amat Azlan, Muhammad Rashid Azrolharith, Innocent Damudu Peter and Falah Hasan Ali Baiee
Veterinary World, 10(7): 779-785

Wan-Nor Fitri: Department of Veterinary Clinical Studies, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
Haron Wahid: Department of Veterinary Clinical Studies, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia; Research Centre for Wildlife, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
Yusoff Rosnina: Department of Veterinary Clinical Studies, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
Faez Firdaus Abdullah Jesse: Department of Veterinary Clinical Studies, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia; Research Centre for Ruminant Diseases, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
Zainal Abidin Aimi-Sarah: Food Science and Technology Program School of Industrial Technology, Faculty of Applied Science, Universiti Teknologi Mara (UiTM), 40450 Shah Alam, Selangor, Malaysia.
Mohd Lila Mohd-Azmi: Department of Veterinary Pathology and Microbiology, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
Che' Amat Azlan: Department of Veterinary Clinical Studies, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
Muhammad Rashid Azrolharith: Department of Medicine & Surgery of Farm & Exotic Animal, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
Innocent Damudu Peter: Department of Veterinary Clinical Studies, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
Falah Hasan Ali Baiee: Department of Veterinary Clinical Studies, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.

doi: 10.14202/vetworld.2017.779-785

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Article history: Received: 29-11-2016, Accepted: 01-06-2017, Published online: 15-07-2017

Corresponding author: Haron Wahid

E-mail: wahidh@upm.edu.my

Citation: Fitri WN, Wahid H, Rosnina Y, Jesse FFA, Aimi-Sarah ZA, Mohd-Azmi ML, Azlan CA, Azrolharith MR, Peter ID, Baiee FHA (2017) Semen characteristics, extension, and cryopreservation of Rusa deer (Rusa timorensis), Veterinary World, 10(7): 779-785.
Abstract

Aim: The objective of this research is to report parameters for breeding soundness evaluation, semen extension, and cryopreservation in Rusa timorensis.

Materials and Methods: Seven healthy stags were chosen for semen collection using an electroejaculator. The collections were performed twice in a breeding season between February and June 2016. Samples were collected between 2 and 3 weeks interval, collected twice for each animal. Semen was evaluated, extended, and cryopreserved using four different extenders; Andromed®, BioXcell®, Triladyl®, and a modified Tris-egg yolk combined with Eurycoma longifolia Jack.

Results: R. timorensis semen characteristics according to volume (ml), color, sperm concentration (106/ml), general motility (%), progressive motility (%), and % morphology of normal spermatozoa are 0.86±0.18 ml, thin milky to milky, 1194.2±346.1 106/ml, 82.9±2.8%, 76.1±4.8%, and 83.9±4.8%, respectively.

Conclusion: Semen characteristics of R. timorensis collected by electroejaculation is good allowing for cryopreservation and future artificial insemination work. The most suitable extender for Rusa deer semen is Andromed®.

Keywords: cryopreservation, electroejaculation, extension, Rusa deer, semen characteristics.

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