Genetic characterization of 11 microsatellite loci in Egyptian pigeons (Columba livia domestica) and their cross-species amplification in other Columbidae populations

Research (Published online: 17-04-2018)

14. Genetic characterization of 11 microsatellite loci in Egyptian pigeons (Columba livia domestica) and their cross-species amplification in other Columbidae populations

Sherif Ramadan, Ahmed Dawod, Osama El-Garhy, Amira M. Nowier, Marwa Eltanany and Miho Inoue-Murayama

Veterinary World, 11(4): 497-505

Sherif Ramadan: Wildlife Research Center, Kyoto University, Kyoto, Japan; Department of Animal Wealth Development, Faculty of Veterinary Medicine, Benha University, Toukh, Egypt.

Ahmed Dawod: Department of Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Sadat City University, Egypt.

Osama El-Garhy: Animal Production Department, Faculty of Agriculture, Benha University, Toukh, Egypt.

Amira M. Nowier: Department of Biotechnology Research, Animal Production Research Institute, ARC, Dokki, Egypt.

Marwa Eltanany: Department of Animal Wealth Development, Faculty of Veterinary Medicine, Benha University, Toukh, Egypt.

Miho Inoue-Murayama: Wildlife Research Center, Kyoto University, Kyoto, Japan; Wildlife Genome Collaborative Research Group, National Institute for Environmental Studies, Tsukuba, Japan.

doi: 10.14202/vetworld.2018.497-505

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Article history: Received: 11-02-2018, Accepted: 19-03-2018, Published online: 17-04-2018

Corresponding author: Miho Inoue-Murayama

E-mail: mmurayama@wrc.kyoto-u.ac.jp

Citation: Ramadan S, Dawod A, El-Garhy O, Nowier AM, Eltanany M, Inoue-Murayama M (2018) Genetic characterization of 11 microsatellite loci in Egyptian pigeons (Columba livia domestica) and their cross-species amplification in other Columbidae populations, Veterinary World, 11(4): 497-505.

Abstract

Aim: This study aimed to analyze the genetic diversity and relationships of 10 Egyptian pigeon populations belonging to Columba livia domestica species using 11 microsatellite markers and to investigate the success of these markers amplification across another eight pigeon species.

Methods: Genomic DNA was isolated from feather samples of 179 pigeon samples from 10 Egyptian breeds: Asfer Weraq (n=14), Austoraly (n=20), Reehani (n=21), Messawed (n=17), Nemssawy (n=27), Otatti (n=12), Morasla (n=17), Tumbler (n=22), Halaby Asfer (n=10), and Karakandy (n=19) in addition to Japanese feral pigeons (n=30). Genotyping was done using 11 specific polymorphic microsatellite makers. Moreover, 37 samples not belonging to C. livia domestica but belonging to another eight pigeon species were genotyped. The polymerase chain reaction (PCR) products were electrophoresed on an ABI 3130xl DNA Sequencer. The basic measures of genetic diversity and phylogenetic trees were computed using bioinformatics software.

Results: Across the 10 studied Egyptian populations, the number of alleles per locus ranged from 3 to 19 and the average number of alleles observed was 9.091. The lowest value of expected heterozygosity (0.373) was obtained for the Reehani breed, and the highest value (0.706) was found for Morasla breed. The overall expected heterozygosity of Egyptian pigeons was 0.548. The F_ST coefficient which indicates fixation coefficients of subpopulations within the total population for the 11 loci varied from 0.318 to 0.114 with a relatively high mean (0.226). In our study, the F_IS showed a relatively high average (0.037). The pairwise Reynolds's genetic distance between the 11 studied pigeon populations recorded lower values between Otatti and Austoraly (0.025) and between Morasla and Japanese feral pigeons (0.054). These results are supported by clustering pattern either by the neighbor-joining phylogenetic tree or by a Bayesian clustering of STRUCTURE with the admixture method.

Conclusion: We confirm the applicability of the CliμD17, CliμT17, CliμD16, CliμD32, CliμT13, CliμD01, PG1, PG2, PG4, PG6, and PG7 microsatellite markers among Egyptian domestic pigeons and across other pigeon species using cross-species amplification method. The information from this study should be useful for genetic characterization and for developing conservation programs of this important species.

Keywords: Egyptian breed, genetic diversity, microsatellite, pigeon.

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