Aim: This study aims to examine the toxicity of collagen extracted from gouramy fish scales (Oshpronemus gouramy) by evaluating its viability against baby hamster kidney fibroblasts-21.

Materials and Methods: Collagen was extracted from gouramy fish scales (O. gouramy) with 6% acetic acid. Its results were analyzed using Fourier-transform infrared spectroscopy and freeze-dried technique. Its morphology then was analyzed with scanning electron microscope. Afterward, 3-(4.5-dimethylthiazole-2-yl)2.5-diphenyl tetrazolium bromide assay was conducted to compare cells with and without fish scale collagen treatment.

Results: Collagen extracted from gouramy fish scales had no influence statistically on cultured fibroblast cells with a statistical significance (2-tailed) value of 0.754 (p>00025).

Conclusion: Collagen extracted from gouramy fish scales has high viability against BHK21 fibroblast cells.

Keywords: bone graft, collagen, gouramy fish scale, viability, 3-(4.5-dimethylthiazole-2-yl)2.5-diphenyl tetrazolium bromide.

1. Newman, M., Takei, H., Klokkevold, P. and Carranza, F. (2012) Carranza's Clinical Periodontology. 11th ed., Vol. 148, Elsevier Saunders, Missouri. p576.

2. Cochran, D.L. (2008) Inflammation and bone loss in periodontal disease. J. Periodontol., 79: 1569-1576. [Crossref] [PubMed]

3. Siaili, M., Chatzopoulou, D. and Gillam, D.G. (2012) An introduction to periodontal regeneration. Dent. Nurs., 9: 686. [Crossref]

4. Dumitrescu, A.L. (2011) Bone grafts and bone grafts substitutes in periodontal therapy. Chemicals in Surgical Periodontal Therapy. Springer, Berlin. p115-116.

5. Jimi, E., Hirata, S., Osawa, K., Terashita, M., Kitamura, C. and Fukushima, H. (2012) The current and future therapies of bone regeneration to repair bone defects. Int. J. Dent., 2012: 1-7. [Crossref] [PubMed] [PMC]

6. Parenteau-Bareil, R., Gauvin, R. and Berthod, F. (2010) Collagen-based biomaterials for tissue engineering applications. Materials (Basel), 3: 1863-87. [Crossref] [PMC]

7. Pati, F., Datta, P., Adhikari, B., Dhara, S., Ghosh, K. and Mohapatra, P. (2012) Collagen scaffolds derived from freshwater fish origin and their biocompatibility. J Biomed Mater Res Part A, 100: 1068-1079. [Crossref] [PubMed]

8. Zhang, F., Wang, A., Li, Z., He, S. and Shao, L. (2011) Preparation and characterisation of collagen from freshwater fish scales. Food Nutr. Sci., 2: 818-23. [Crossref]

9. Him. (2007) Gurami Masih Unggul (Gouramy is still superior). Vol. 51. Warta Pasar Ikan (Fish Market News). p16-7.

10. Kasim, S. (2013) The effect of acid solvent variation on collagen extraction from stingray (Himantura gerrardi) and tuna (Thunnus sp). Pharm. Pharmacol. J., 17: 35-38.

11. Yuliati, A. (2005) Viabilitas sel fibroblas BHK-21 pada permukaan resin akrilik rapid heat cured (Viability of fibroblast BHK-21 cells to the surface of rapid heat cured acrylic resins). Dent. J. (Majalah Kedokt Gigi), 38: 68-72. [Crossref]

12. Siregar, F. and Hadijono, B.S. (2000) Uji Sitotoksisitas dengan Esei MTT (Cytotoxicity Test with an MTT Assay). J. Kedokt. Gigi. UI, 7: 28-32.

13. Bharatham, B.H., Bakar, M.Z.A., Perimal, EK., Yusof, L.M. and Hamid, M. (2014) Development and characterization of novel porous 3d alginate-cockle shell powder nanobiocomposite bone scaffold. Biomed. Res. Int., 2014: 1-11. [Crossref] [PubMed] [PMC]

14. Yamada, S., Yamamoto, K., Ikeda, T., Yanagiguchi, K. and Hayashi, Y. (2014) Potency of fish collagen as a scaffold for regenerative medicine. Biomed. Res. Int., 3: 1-7. [Crossref] [PubMed] [PMC]

15. Erizal, E., Perkasa, D.P. and Abbas, B. (2013) Synthesis of fish scales gelatin-chitosan crosslinked films by gamma irradiation techniques. Sci. J. Appl. Isot. Radiat., 9: 101-112.

16. Junior, Z.S.S., Botta, S.B., Ana, P.A., Franca, C.M., Fernandes, K.P.S. and Mesquita-Ferrari, R.A. (2015) Effect of papain-based gel on Type I collagen--spectroscopy applied for microstructural analysis. Sci. Rep., 5: 1-7. [Crossref] [PubMed] [PMC]

17. Kiew, P.L. and Mat Don, M. (2013) The influence of acetic acid concentration on the extractability of collagen from the skin of Hybrid clarias sp. and its physicochemical properties?: A preliminary study. Focus Mod. Food Ind., 2: 123-128.

18. Zheng, W., Zhang, W. and Jiang, X. (2010) Biomimetic collagen nanofibrous materials for bone tissue engineering. Adv. Eng. Mater., 12: 461-466. [Crossref]

19. Alvarez, K. and Nakajima, H. (2009) Metallic scaffolds for bone regeneration. Materials (Basel), 2: 790-832. [Crossref] [PMC]

20. da Cruz, A.C.C., Pochapski, M.T., Daher, J.B., da Silva, J.C.Z., Pilatti, G.L. and Santos, F.A. (2006) Physico-chemical characterization and biocompatibility evaluation of hydroxyapatites. J. Oral. Sci., 48: 219-226. [Crossref] [PubMed]

21. Larjava, H. (2012) Oral Wound Healing. John Wiley & Sons, New York. p195-223. [Crossref]

22. Meizarini, A. (2005) Cytotoxicity of the cyanoacrylate restoration material with variation of powder and liquid ratio by using MTT assay. Dent. J. (Majalah Kedokt Gigi) 38: 20-24. [Crossref]

23. Wijaya, J., Salenussa, J. and Marantika, J. (2013) Potential of lime leaf methanol extract (Harmsiopanax aculeatus, Harms) as antimalarial drugs. Prog. Kreat Mhs Penelit., 8: 1-9.

24. Telli, C., Serper, A., Dogan, A.L. and Guc, D. (1999) Evaluation of the cytotoxicity of calcium phosphate root canal sealers by MTT assay. J. Endod., 25: 811-813. [Crossref]

25. Leong, L.M., Sahalan, A.Z., Tan, L.H., Mustafa, N.H. and Rajab, N.F. (2015) Clarias batrachus collagen extract increases fibroblast cell adhesion, migration and proliferation. J. Appl. Pharm. Sci., 5: 19-23. [Crossref]

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