Aim: The study was carried out to determine the seroprevalence of Brucella antibody in slaughter cattle and goats; and the role of slaughterhouse workers (SHWs) in spread of Brucella infection during slaughterhouse operations in Enugu State, Southeast Nigeria.

Materials and Methods: Rose Bengal plate test was used to screen for Brucella antibody in 484 cattle and 340 goats slaughtered for human consumption in the state. Structured and pretested questionnaire was used to elicit information from randomly selected SHWs, on socioeconomic characteristics, awareness of brucellosis and involvement in practices that aid dissemination of Brucella infection during slaughterhouse operations.

Results: Suspected seroprevalence of 2.5% and 4.1% were recorded for Brucella antibody in cattle and goats respectively. There was poor awareness of brucellosis (32.1%) among the workers surveyed. Slaughterhouse practices that aid acquisition or spread of Brucella infection and percentage of SHWs engaged in the practices are: non-use of personal protective clothing during slaughterhouse operations (70.8%), discharge of eviscerated fetuses or pregnant uterine contents by open-air dump method of refuse disposal (64.9%) and illegal sell of eviscerated fetuses or gravid uterine contents for human consumption (59.9%) or preparation of dog food (71.5%).

Conclusion: The 4.1% suspected seroprevalence of Brucella antibodies in goats represents 128% increase from 1.8% seroprevalence earlier reported in the same species and study area in 2009. Significant amounts of Brucella antibody was detected in the food animals screened. Slaughterhouse workers played significant roles in spread of Brucella infection by their involvement in risk practices and behaviours that facilitate pathogen transmission. Therefore, massive awareness campaign and coordinated brucellosis control program in Enugu State are imperative to forestall the zoonotic and economic consequences associated with brucellosis.

Keywords: Brucella antibody, brucellosis, cattle, goats, slaughterhouse workers.

1. Godfroid, J., Bastuji, B.G. and Saegerman, C. (2013) Brucellosis in terrestrial wildlife. Rev. Sci. Technol., 32: 27-42. [Crossref] [PubMed]

2. Ducrotoy, M., Bertu, W.J., Matope, G., Cadmus, S., Conde-Alvarez, R., Gusi, A.M., Welburn, S., Ocholi, R., Blasco, J.M. and Moriyon I. (2017) Brucellosis in Sub-Saharan Africa: Current challenges for management, diagnosis and control. Acta Tropica., 165: 179-193. [Crossref]

3. Godfroid, J., Scholz, H.C., Barbier, T., Nicolas, C., Wattiau, P., Fretin, D., Whatmore, A.M., Cloeckaert, A., Blasco, J.M., Moriyon, I., Saegerman, C., Muma, J.B., AlDahouk, S., Neubauer, H. and Letesson, J.J. (2011) Brucellosis at the animal/ecosystem/human interface at the beginning of the 21st century. Prev. Vet. Med., 102: 118-131. [Crossref] [PubMed]

4. Lindahl, E., Sattorov, N., Boqvist, S., Sattori, I. and Magnusson, U. (2014) Seropositivity and risk factors for Brucella in dairy cows in urban and peri-urban small-scale farming in Tajikistan. Trop. Anim. Health Prod., 46: 563-569. [Crossref] [PubMed] [PMC]

5. Ogugua, A.J., Akinseye, O.V., Ayoola, M.C., Stack, J. and Cadmus, S.I.B. (2015) Risk factors associated with brucellosis among slaughtered cattle: Epidemiological insight from two metropolitan abattoirs in Southwestern Nigeria. Asian Pac. J Trop. Dis., 5: 747-753.

6. Onunkwo, J.I., Njoga, E.O., Nwanta, J.A., Shoyinka, S.V.O., Onyenwe, I.W. and Eze J.I. (2011) Serological survey of porcine Brucella infection in Southeast, Nigeria. Nigerian Vet. J., 32: 60-62. [Crossref]

7. McDermott, J., Grace, D. and Zinsstag, J. (2013) Economics of brucellosis impact and control in low-income countries. Rev. Sci. et Tech., 32: 249-261. [Crossref] [PubMed]

8. Brisibe, F., Nawathe, D.R. and Bot, C.J. (1996) Sheep and goat brucellosis in Borno and Yobe States of arid Northeastearn Nigeria. Small Rum. Res., 20: 83-88. [Crossref]

9. Bamaiyi, P.H., Khairani-Bejo, S. and Zainal-abidin, M. (2015) The economic impact attributable to brucellosis among goat farms in Peninsula Malaysia and cost benefit analysis. Res Opin. Anim. Vet. Sci., 5: 57-64.

10. Ducrotoy, M.J., Bertu, W.J., Ocholi, R.A., Gusi, A.M., Bryssinckx, W., Welburn, S. and Moriyon I. (2014) Brucellosis as an emerging threat in developing economies: Lessons from Nigeria. PLoS Negl. Trop. Dis., 8: e3008. [Crossref]

11. Rossetti, C.A., Arenas-Gamboa, A.M. and Maurizio, E. (2017) Caprine brucellosis: A historically neglected disease with significant impact on public health. PLoS Negl. Trop. Dis., 11: e0005692. [Crossref]

12. Onunkwo, J.I., Njoga, E.O., Njoga, U.J., Ezeokafor, E, Ekere, S.O (2018) Brucella seropositivity in chicken and risk factors for Brucella infection at the animal-human interface in Anambra State, Nigeria. Int. J. One health 4:28-34. [Crossref]

13. Boral, R., Singh, M. and Singh, D.K. (2009) Status and strategies for control of brucellosis - a review. Indian J. Anim. Sci., 79: 1191-1199.

14. Njoga, E.O., Onunkwo, J.I., Ekere, S.O., Njoga, U.J. and Okoro, W.N (2018) Seroepidemiology of equine brucellosis and role of horse carcass processors in spread of Brucella infection in Enugu State, Nigeria. Int. J. Curr. Res. Rev., 10:39-45. [Crossref]

15. Rajala, E.L., Grahn, C., Ljung, I., Sattorov, N., Boqvist, S. and Magnusson, U. (2016) Prevalence and risk factors for Brucella seropositivity among sheep and goats in a peri-urban region of Tajikistan. Trop. Anim. Health Prod., 48, 553-558. [Crossref] [PubMed] [PMC]

16. Adamu, S.G., Atsanda, N.N., Tijjani, A.O., Usur, A.M., Sule, A.G. and Gulani I.A. (2016) Epidemiological study of bovine brucellosis in three senatorial zones of Bauchi State, Nigeria. Vet. World, 9: 48-52. [Crossref] [PubMed] [PMC]

17. Adamu, N.B., Adeniyi, S.O., Adamu, S.G., Bale, J.O.O., Okoh, A.E.J., Umaru, G.A. and Umar Y.A. (2015) Seroprevalence of brucellosis among livestock workers at Maiduguri cattle market, Borno State, North Eastern, Nigeria. J. Public Health Epidemiol., 7, 253-257. [Crossref]

18. Dean, A.S., Crump, L., Greter, H., Schelling, E. and Zinsstag, J (2012) Global burden of human brucellosis: A systematic review of disease frequency. PLoS Negl.Trop. Dis., 6: e1865. [Crossref]

19. Cadmus, S.I.B., Ijagbone, I.F., Oputa, H.E. and Adesokan, H.K. (2013) Serological survey of brucellosis in livestock animals and workers in Ibadan, Southwestern, Nigeria. Africa J. Biomed.Res., 9: 163-168.

20. Adesokan, H.K., Alabi, P.I., Stack, J.A. and Cadmus, S.I.B. (2013) Knowledge and practices related to bovine brucellosis transmission amongst livestock workers in Yewa, South-western Nigeria. J. South Africa Vet. Asso, 84: 121-125. [Crossref] [PubMed]

21. Kaltungo, B.Y., Saidu, S.N.A., Sackey, A.K.B. and Kazeem, H.M. (2014) A review on diagnostic techniques for brucellosis. African J. Biotech, 13: 1-10. [Crossref]

22. Junaidu, A.U., Oboegbulem, S.I. and Salihu, M.D. (2010) Seroprevalence of brucellosis in prison farms in Sokoto State, Nigeria. Asian J. Epidemiol., 3: 107-111.

23. Mai, H.M., Irons, P.C., Junaidu, K. and Thompson, P.N. (2012) A large seroprevalence survey of brucellosis in cattle herds under diverse production systems in northern Nigeria. BMC Vet. Res., 8: 1-14. [Crossref] [PubMed] [PMC]

24. Ijale, G.O., Ajogi, I. and Dzikwi, A.A. (2014) Seroprevalence of Brucella antibodies in goats in Oju, Benue State, Nigeria. Asian J. Epidemiol, 6: 43-48. [Crossref]

25. Njoga, E.O., Onunkwo, J.I., Okoli, C.E., Ugwuoke, W.I., Nwanta, J.A. and Chah, K.F. (2018) Assessment of antimicrobial drug administration and antimicrobial residues in food animals in Enugu State, Nigeria. Trop. Anim. Health Prod., 50: 897-902. [Crossref] [PubMed]

26. Pace, J.E. and Wakeman, D.L. (2003) Determining the Age of Cattle by Their Teeth, University of Florida, IFAS Extension. Available from: http://www.edis.ifas.ufl.edu. Retrieved on 6-01-2017.

27. Amin, M.M., Ahmed, S.A., Zaki, H.M. and Ismail, R.I. (2012) Serological and molecular studies on the diagnosis of bovine brucellosis. Nat. Sci., 10: 68-76.

28. Negash, E., Shimelis, S. and Beyene, D. (2012) Seroprevalence of small ruminant brucellosis and its public health awareness in selected sites of Dire Dawa region, Eastern Ethiopia. J. Vet. Med. Anim. Health., 4: 61-66.

29. Kaushik, P., Kumar, M.L., Anjay, S.K. and Kumar P. (2016) Sero-prevalence of bovine brucellosis in Bihar, India. J Vet. Pub. Health, 14: 51-53.

30. Onunkwo, J.I., Nwanta, J.A., Ezenduka, E.V., Oboegbulem, S.I. and Onyenwe, I.W. (2009) Seroepidemiological survey of Brucella infection in slaughterhouse goats in Enugu State of Southeastern Nigeria. Anim. Sci. Reporter., 3: 123-127.

31. Petersen, E., Rajashekara, G., Sanakkayala, N., Eskra, L., Harms, J. and Splitter, G. (2013) Erythritol triggers expression of virulence traits in Brucella melitensis. Microb. Infect, 15: 440-449. [Crossref] [PubMed] [PMC]

32. Kracalik, I.T., Abdullayev, R., Asadov, K., Ismayilova, R., Baghirova, M., Ustun, N., Shikhiyev, M., Talibzade, A. and Blackburn, J.K (2016) Human brucellosis trends: Re-emergence and prospects for control using a one health approach in Azerbaijan (1983-2009). Zoonoses Public Health, 63: 294-302. [Crossref] [PubMed]

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