Aim: The aim of the present study was to investigate the effect of supplementation of multistrain probiotic preparation in combination with vitamins and minerals to the broiler chicken diets on their growth performance, hematological parameters, and carcass traits.

Materials and Methods: Two hundred and eighty-eight Lohmann 1-day-old broiler chicks were randomly allocated to four groups, i.e., control (without additional supplementation) and three experimental treatments where basal diet was enriched by 0.1%, 0.5%, or 1% of multistrain probiotic preparation in combination with vitamins and minerals, respectively. Blood sampling was conducted on day 28, while the selected organs and eviscerated carcasses were collected on day 42.

Results: Dietary supplementation did not affect (p>0.05) the final body weight, feed intake, and feed conversion ratio of broilers. Supplementation by 0.1% and 0.5% of multistrain probiotic preparation in combination with vitamins and minerals reduced (p≤0.05) heart relative weight of broilers. Dietary supplementation tended (p=0.07) to increase the relative weight of ileum and pancreas of broilers. Supplemented birds had lower (p≤0.05) numbers of leukocytes and eosinophils compared to unsupplemented birds. There were tendencies that supplementation of multistrain probiotics in combination with vitamins and minerals resulted in lower (p=0.07) counts of lymphocytes and heterophils when compared with no supplementation. Supplementation by 0.5% of multistrain probiotics in combination with vitamins and minerals resulted in lower (p≤0.05) serum concentration of uric acid when compared with control. There was no significant effect of dietary supplementation on carcass traits, pH, and drip loss of broiler breast muscles.

Conclusion: Dietary supplementation of commercial broiler feeds with 0.5% of multistrain probiotic preparation in combination with vitamins and minerals was potential to improve digestive functions and physiological status of broiler chickens.

Keywords: broiler chicks, growth rate, minerals, multistrain probiotics, physiological status, vitamins.

1. Aviagen. (2014) Ross Broiler Management Handbook. Available from: http://www.aviagen.com. Accessed on 07-07-2017.

2. Rahman, M.A., Parvin, M.S., Sarker, R.R. and Islam, M.T. (2012) Effects of growth promoter and multivitamin-mineral premix supplementation on body weight gain in broiler chickens. J. Bangl. Agric. Univ., 10: 245-248.

3. Anjum, M.I., Khan, A.G., Azim, A. and Afzal, M. (2005) Effect of dietary supplementation of multi-strain probiotic on broiler growth performance. Pak. Vet. J., 25: 25-29.

4. Islam, M.S., Bhuiyan, M.E.R., Begum, M.I.A., Miah, M.A. and Myenuddin, M. (2004) Effect of vitamin-mineral premix supplementation on body weight and certain haemato-biochemical values in broiler chickens. Bangl. J. Vet. Med., 2: 45-48.

5. Ajuwon, O.R., Idowu, O.M.O., Afolabi, S.A., Kehinde, B.O., Oguntola, O.O. and Olatunbosun, K.O. (2011) The effects of dietary copper supplementation on oxidative and antioxidant systems in broiler chickens. Arch. Zoo., 60: 275-282. [Crossref]

6. Harun-Ar-Rashid, M., Ahmad, N., Amin, M.R. and Mollah, M.L. (2015) Effects of selected vitamins and minerals on growth rate and hematological parameters in broilers. Asian J. Med. Biol. Res., 1: 487-494. [Crossref]

7. Etela, I., Kalio, G.A., Monsi, A. and Ezieshi, E.V. (2007) Feed intake, growth rate and some anatomical characteristics of broilers fed commercial diets supplemented with green feeds. Renew. Agric. Food Syst., 22: 241-245. [Crossref]

8. Fernandes, J.I.M., Bortoluzzi, C., Junior, A.M.B., Rorig, A., Perini, R. and de Cristo, A.B. (2015) Effect of different enzymatic supplements in diets of broilers raised at high stocking density. J. Vet. Med. Res., 2: 1-4.

9. Murakami, A.E., Fernandes, J.I.M., Hernandes, L. and Santos, T.C. (2012) Effects of starter diet supplementation with arginine on broiler production performance and on small intestine morphometry. Pesq. Vet. Bras., 32: 259-266. [Crossref]

10. Sanda, M.E. (2015) Effects of vitamin-mineral supplement on the immune response of broilers to Newcastle disease vaccination. Int. J. Agric. Vet. Sci., 1: 10-13. [Crossref]

11. Sugiharto, S. (2016) Role of nutraceuticals in gut health and growth performance of poultry. J. Saudi Soc. Agric. Sci., 15: 99-111. [Crossref]

12. Fouad, A.M. and El-Senousey, H.K. (2014) Nutritional factors affecting abdominal fat deposition in poultry: A review. Asian Aust. J. Anim. Sci., 27: 1057-1068. [Crossref] [PubMed] [PMC]

13. Shabani, R., Nosrati, M., Javandel, F. and Kioumarsi, H. (2012) The effect of probiotics on carcass and internal organs of broilers. Ann. Biol. Res., 3: 5475-5477.

14. Zaboli, G.R., Bilondi, H.H. and Miri, A. (2013) The effect of dietary antioxidant supplements on abdominal fat deposition in broilers. Life Sci. J., 10: 328-333.

15. Albuquerque, D.M.N., Lopes, J.B., Ferraz, M.S., Ribeiro, M.N., Silva, S.R.G., Costa, E.M.S., Lima, D.C.P., Ferreira, J.D.M., Gomes, P.E.B. and Lopes, J.C.O. (2017) Vitamin E and organic selenium for broilers from 22 to 42 days old: Performance and carcass traits. Anais Acad. Bras. Cienc., 89: 1259-1268. [Crossref] [PubMed]

16. Kucuk, O., Sahin, N. and Sahin, K. (2003) Supplemental zinc and vitamin A can alleviate negative effects of heat stress in broiler chickens. Biol. Trace Elem. Res., 94: 225-235. [Crossref]

17. Lu, L., Jia, C., Luo, X.G., Liu, B. and Yu, S.X. (2006) The effect of supplemental manganese in broiler diets on abdominal fat deposition and meat quality. Anim. Feed Sci. Technol., 129: 49-59. [Crossref]

18. Ghosh, A., Mandal, G.P., Roy, A. and Patra, A.K. (2016) Effect of supplementation of manganese with or without phytase on growth performance, carcass traits, muscle and tibia composition, and immunity in broiler chickens. Livest. Sci., 191: 80-85. [Crossref]

19. Nikoofard, V., Mahdavi, A.H., Samie, A.H. and Jahanian, E. (2016) Effects of different sulphur amino acids and dietary electrolyte balance levels on performance, jejunal morphology, and immunocompetence of broiler chicks. J. Anim. Physiol. Anim. Nutr., 100: 189-199. [Crossref] [PubMed]

20. Yang, X.J., Sun, X.X., Li, C.Y., Wu, X.H. and Yao, J.H. (2015) Effects of copper, iron, zinc, and manganese supplementation in a corn and soybean meal diet on the growth performance, meat quality, and immune responses of broiler chickens. J. Appl. Poult. Res., 20: 263-271. [Crossref]

21. Isroli, I., Yudiarti, T., Widiastuti, E. and Sugiharto, S. (2017) Probiotic Bacillus plus vitamins and minerals enhanced haemoglobin values and relative weight of ileum and improved feed conversion ratio of broilers during brooding period. Livest. Res. Rural Dev. Available from: http://www.lrrd.org/lrrd29/11/sgh29212.html. Last accessed on 05-11-2017.

22. Puspani, P., Candrawati, D.P.M.A. and Bidura, I.G.N.G. (2016) Implementation probiotics cellulolitic B-7 bacteria (isolation from buffalo rumen) into rations on the performance, abdominal fat and serum cholesterol of duck. Int. J. Curr. Microbiol. App. Sci., 5: 432-441. [Crossref]

23. Sornplang, P. and Piyadeatsoontorn, S. (2016) Probiotic isolates from unconventional sources: A review. J. Anim. Sci. Technol., 58: 26. [Crossref] [PubMed] [PMC]

24. Sugiharto, S., Yudiarti, T., Isroli, I., Widiastuti, E. and Putra, F.D. (2017) Effect of dietary supplementation with Rhizopus oryzae or Chrysonilia crassa on growth performance, blood profile, intestinal microbial population, and carcass traits in broilers exposed to heat stress. Arch. Anim. Breed., 60: 347-356. [Crossref]

25. Sugiharto, S., Yudiarti, T., Isroli, I., Widiastuti, E. and Putra, F.D. (2017) Effects of feeding cassava pulp fermented with Acremonium charticola on growth performance, nutrient digestibility and meat quality of broiler chicks. S. Afr. J. Anim. Sci., 47: 130-138. [Crossref]

26. Hatab, M.H., Elsayed, M.A. and Ibrahim, N.S. (2016) Effect of some biological supplementation on productive performance, physiological and immunological response of layer chicks. J. Rad. Res. Appl. Sci., 9: 185-192. [Crossref]

27. Aengwanich, W. and Simaraks, S. (2004) Pathology of heart, lung, liver and kidney in broilers under chronic heat stress. Songklanakarin J. Sci. Technol., 26: 417-424.

28. Khan, A.Z., Kumbhar, S., Hamid, M., Afzal, S., Parveen, F., Liu, Y., Shu, H., Mengistu, B.M. and Huang, K. (2016) Effects of selenium-enriched probiotics on heart lesions by influencing the mRNA expressions of selenoproteins and heat shock proteins in heat stressed broiler chickens. Pak. Vet. J., 36: 460-464.

29. Syafwan, S., Kwakkel, R.P. and Verstegen, M.W.A. (2011) Heat stress and feeding strategies in meat-type chickens. World's Poult. Sci. J., 67: 653-673. [Crossref]

30. Olnood, C.G., Beski, S.S.M., Choct, M. and Iji, P.A. (2015) Novel probiotics: Their effects on growth performance, gut development, microbial community and activity of broiler chickens. Anim. Nutr., 1: 184-191. [Crossref]

31. Tsirtsikos, P., Fegeros, K., Balaskas, C., Kominakis, A. and Mountzouris, K.C. (2012) Dietary probiotic inclusion level modulates intestinal mucin composition and mucosal morphology in broilers. Poult. Sci., 91: 1860-1868. [Crossref] [PubMed]

32. Hassanpour, H., Bahadoran, S. and Borjian, N. (2016) Vitamin E improves morphology and absorptive surface of small intestine in broiler chickens reared at high altitude. Poult. Sci. J., 4: 19-26.

33. Read-Snyder, J., Edens, F.W., Cantor, A.H., Pescatore, A.J. and Pierce, J.L. (2009) Effect of dietary selenium on small intestine villus integrity in reovirus-challenged broilers. Int. J. Poult. Sci., 8: 829-835. [Crossref]

34. Olnood, C.G., Beski, S.S.M., Iji, P.A. and Choct, M. (2015) Delivery routes for probiotics: Effects on broiler performance, intestinal morphology and gut microflora. Anim. Nutr., 1: 192-202. [Crossref]

35. Shah, S.N., Kamil, S.A., Darzi, M.M., Mir, M.S. and Bhat, S.A. (2013) Haematological and some biochemical changes in experimental fowl typhoid infection in broiler chickens. Comp. Clin. Pathol., 22: 83-91. [Crossref]

36. Akhtar, M., Awais, M.M., Anwar, M.I., Ehtisham-ul-Haque, S., Nasir, A., Saleemi, M.K. and Ashraf, K. (2015) The effect of infection with mixed Eimeria species on hematology and immune responses following Newcastle disease and infectious bursal disease booster vaccination in broilers. Vet. Quart., 35: 21-26. [Crossref] [PubMed]

37. Allan, W.H. and Gough, R.E. (1974) A standard haemagglutination inhibition test for Newcastle disease. (1). A comparison of macro and micro methods. Vet. Record., 95: 120-123. [Crossref] [PubMed]

38. Sanda, M.E., Ezeibe, M.C.O. and Anene, B.M. (2015) Effects of vitamins A, C and E and selenium on immune response of broilers to Newcastle disease (ND) vaccine. IOSR J. Agric. Vet. Sci., 8: 13-15.

39. El-Katcha, M.I., Soltan, M.A., El-Kaney, H.F. and Karwarie, E.R. (2014) Growth performance, blood parameters, immune response and carcass traits of broiler chicks fed on graded levels of wheat instead of corn without or with enzyme supplementation. Alexandria J. Vet. Sci., 40: 95-111. [Crossref]

40. Lin, H., Huang, Q., Guo, X., Liu, P., Liu, W., Zou, Y., Zhu, S., Deng, G., Kuang, J., Zhang, C., Cao, H. and Hu, G. (2015) Elevated level of renal xanthine oxidase mRNA transcription after nephropathogenic infectious bronchitis virus infection in growing layers. J. Vet. Sci., 16: 423-429. [Crossref] [PubMed] [PMC]

41. Kalorey, D.R., Kurkure, N.V., Ramgaonkar, J.S., Sakhare, P.S., Warke, S. and Nigot, N.K. (2005) Effect of polyherbal feed supplement "Growell" during induced aflatoxicosis, ochratoxicosis and combined mycotoxicoses in broilers. Asian Aust. J. Anim. Sci., 18: 375-383. [Crossref]

42. Ridi, R.E. and Tallima, H. (2017) Physiological functions and pathogenic potential of uric acid: A review. J. Advan. Res., 8: 487-493. [Crossref] [PubMed] [PMC]

43. Nery, R.A., Kahlow, B.S., Skare, T.L., Tabushi, F.I. and do Amaral, C.A. (2015) Uric acid and tissue repair. Arq. Bras. Cirurgia Digest., 28: 290-292. [Crossref] [PubMed] [PMC]

44. Knap, I., Kehlet, A.B., Bennedsen, M., Mathis, G.F., Hofacre, C.L., Lumpkins, B.S., Jensen, M.M., Raun, M. and Lay, A. (2011) Bacillus subtilis (DSM17299) significantly reduces Salmonella in broilers. Poult. Sci., 90: 1690-1694. [Crossref] [PubMed]

45. Toghyani, M., Toghyani, M. and Tabeidian, S.A. (2011) Effect of probiotic and prebiotic as antibiotic growth promoter substitutions on productive and carcass traits of broiler chicks. International Conference on Food Engineering and Biotechnology. IPCBEE. Vol. 9. IACSIT Press, Singapore.

46. Hascik, P., Trembecka, L., Bobko, M., Kacaniova, M., Cubon, J., Kunova, S. and Bucko, O. (2016) Effect of diet supplemented with propolis extract and probiotic additives on performance, carcass characteristics and meat composition of broiler chickens. Potravinarstvo, 10: 223-231. [Crossref]

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