Aim: The experiment was conducted to determine of levamisole (0.2 ml/kg-BW), Vitamin E (80 mg)+selenium (1.6 mg), and aflatoxin (B1) (positive control) compared with group without aflatoxin (negative control) on some liver enzymes (aspartate transaminase [AST] and alanine transaminase [ALT]), as well as to study the histopathological changes.

Materials and Methods: The experiment included (200) 1-day-old broilers Ross 308 (Turkey source) mixed sexes. They were divided into four equal groups (50 chicks each group). The experimental period was extended to 35 days.

Results: The results revealed that the levels of liver enzymes (ALT and AST) of all groups at 35 days were significantly (p<0.05) higher than the negative control. Furthermore, the result of histopathological changes in thymus and Harderian gland in different ages of group Vitamin E+selenium showed a reduction in the depletion of the cortex as well as lessening of congestion and hemorrhage and necrosis also decreasing in inflammatory cells in the thymus and Harderian gland.

Conclusion: The study confirmed the protective effect of Vitamin E and levamisole by reducing harmful impacts of aflatoxin through their antioxidant effect as they improved the liver enzymes and histopathological changes due to the toxin.

Keywords: aflatoxin, aspartate transaminase, alanine transaminase, broiler, levamisole, Vitamin E and selenium.

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