doi: 10.14202/vetworld.2018.125-129
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Article history: Received: 20-09-2017, Accepted: 27-12-2017, Published online: 06-02-2018
Corresponding author: Myassar O. Alekish
E-mail: moalekish@just.edu.jo
Citation: Alekish MO, Ismail ZB, Hammouri HM, Daradka MH, Taha SA, Olymat I (2018) Bacteriological cure rate and changes in milk composition in mastitis vaccinated ewes affected with subclinical mastitis, Veterinary World, 11(2): 125-129.Aim: The aim of this study was to investigate the effects of using a commercially-available polyvalent mastitis vaccine on the bacteriological cure rate of existing subclinical mastitis in Awassi sheep.
Materials and Methods: A total of 164 lactating ewes were divided into two main groups according to udder health and milk somatic cell count (SCC): Group 1=normal (N; n=80) and Group 2=subclinical mastitis (SC; n=84). Each group was then subdivided randomly into two treatment groups: N vaccinated (Nvax; n=38), N non-vaccinated (Nnvax; n=42), SC vaccinated (SCvax; n=42), and SC non-vaccinated (SCnvax; n=42). The vaccine was administered as per manufacturer's recommendations. Milk samples were collected aseptically from all ewes before vaccine administration (T0) and again on days 28 (T2) and 42 (T3) of the experiment.
Results: In the SC group, the bacteriological cure rates in vaccinated and non-vaccinated ewes were 76% and 69%, respectively. In N group, the new intramammary infection rates in vaccinated and non-vaccinated ewes were 48% and 50%, respectively. Vaccination of normal ewes resulted in a significant (p<0.05) reduction in bacterial growth rate both at day 28 and day 42 of the study. The prevalence of new intramammary infection rate in Nvax ewes on days 28 and 42 was 19% and 20%, respectively. The prevalence of new intramammary infection rate in Nnvax group on days 28 and 42 was 33% and 30%, respectively. In SCvax group, the bacterial growth rate on days 28 and 42 was 44% and 35%, respectively. In SCnvax group, the bacterial growth rate on days 28 and 42 was 27% and 32%, respectively. There was no statistically significant effect of vaccination on any of the studied milk composition parameters.
Conclusion: This is a preliminary study that indicated a possible protective effect of vaccination against mastitis in sheep. Further, case-controlled studies are indicated to estimate the level of immunity this vaccine provides to vaccinated sheep.
Keywords: immunity, mastitis pathogens, prevention, vaccination.
1. Bergonier, D. and Berthelot, X. (2003) New advances in epizootiology and control of ewe mastitis. Livest. Prod. Sci., 79: 1-16. [Crossref]
2. Contreras, A., Sierra, D., Sanches, A., Corrales, J.C., Marco, J.C., Paape, M.J. and Gonzalo, C. (2007) Mastitis in small ruminant. Small Anim. Res., 68: 145-153. [Crossref]
3. Sharif, A. and Muhammad, G. (2009) Mastitis control in dairy animals. Pak. Vet. J., 29: 145-148.
4. De Vliegher, S., Fox, L.K., Piepers, S., McDougall, S. and Barkema, H.W. (2012) Mastitis in dairy heifers: Nature of the disease, potential impact, prevention, and control. J. Dairy Sci., 95: 1025-1040. [Crossref] [PubMed]
5. Cha, E., Bar, D., Hertl, J.A., Tauer, L.W., Bennett, G., Gonzalez, R.N., Schukken, Y.H., Welcome, F.L. and Grohn, Y.T. (2011) The cost and management of different types of clinical mastitis in dairy cows estimated by dynamic programming. J. Dairy Sci., 94: 4476-4487. [Crossref] [PubMed]
6. Leitner, G., Lubashevsky, E., Glickman, A., Winkler, M., Saran, A. and Trainin, Z. (2003) Development of a Staphylococcus aureus vaccine against mastitis in dairy cows. Challenge trials. Vet. Immunol. Immunopathol., 93: 31-38. [Crossref]
7. Hadimli, H.H., Erganis, O., Kav, K. and Sayin, Z. (2005) Evaluation of a combined vaccine against staphylococcal mastitis in ewes. Bull. Vet. Inst. Pulawy., 49: 179-182.
8. Suojala, L., Simojoki, H., Mustonen, K., Kaartinen, L. and Pyoraat, S. (2010) Efficacy of enrofloxacin in the treatment of naturally occurring acute clinical Escherichia coli mastitis. J. Dairy Sci., 93: 1960-1969. [Crossref] [PubMed]
9. Pellegrino, M., Giraudo, J., Raspanti, C., Nagel, R., Odierno, L. Primo, V. and Bogni, C. (2008) Experimental trial in heifers vaccinated with Staphylococcus aureus a virulent mutant against bovine mastitis. Vet. Microbiol., 127: 186-190. [Crossref] [PubMed]
10. Gurjar, A.A., Klaessig, S., Salmon, A.A., Yancey, R.J. Jr. and Schukken, Y.H. (2013) Evaluation of an alternative dosing regimen of a J-5 mastitis vaccine against intramammary Escherichia coli challenge in non-lactating late-gestation dairy cows. J. Dairy Sci., 96: 5053-5063. [Crossref] [PubMed]
11. Schukken, Y.H., Bronzo, V., Locatelli, C., Pollera, C., Rota, N., Casula, A., Testa, F., Scaccabarozzi, L., March, R., Zalduendo, D., Guix, R. and Moroni, P. (2014) Efficacy of vaccination on Staphylococcus aureus and coagulase-negative staphylococci intramammary infection dynamics in 2 dairy herds. J. Dairy Sci., 97: 5250-5264. [Crossref] [PubMed]
12. Halasa, T., Huijps, K., Osteras, O. and Hogeveen, H. (2007) Economic effects of bovine mastitis and mastitis management: A review. Vet. Q., 29: 18-31. [Crossref]
13. Dohoo, I.R., Smith, J., Andersen, S., Kelton, D.F. and Godden, S. (2011) Diagnosing intramammary infections: Evaluation of definitions based on a single milk sample. J. Dairy Sci., 94: 250-261. [Crossref]
14. Middleton, J.R., Ma, J., Rinehart, C.L., Taylor, V.N., Luby, C.D. and Steevens, B.J. (2006) Efficacy of different lysigin formulations in the prevention of Staphylococcus aureus intramammary infection in dairy heifers. J. Dairy Res., 73:10-19. [Crossref] [PubMed]
15. Middleton, J.R., Luby, C.D. and Adams, D.S. (2009) Efficacy of vaccination against staphylococcal mastitis: A review and new data. Vet. Microbiol., 134: 192-198. [Crossref] [PubMed]
16. Bradley, A.J., Breen, J.E., Payne, B., White, V. and Green, M.J. (2015) An investigation of the efficacy of a polyvalent mastitis vaccine using different vaccination regimens under field conditions in the United Kingdom. J. Dairy Sci., 98: 1706-1720. [Crossref]
17. Barillet, F., Rupp, R., Miganon-grasteau, S., Astruc, J.M. and Jacquin, M. (2001) Genetic analysis for mastitis resistance and milk somatic cell score in French Lacaune dairy sheep. Genet. Select. Evol., 33: 397-415. [Crossref] [PubMed] [PMC]
18. Gonzalo, C., Ariznabarreta, A., Carriedo, J.A. and San Primitivo, F. (2002) Mammary pathogens and their relationship with somatic cell count and milk yield losses in dairy ewes. J. Dairy Sci., 85: 1460-1467. [Crossref]
19. Pradiee, J., Moraes, C., Goncalves, M., Vilanova, M.S., Correa, G.F., Lauz, O.G., Osorio, M.T.M. and Schmidt, V. (2012) Somatic cell count and California mastitis test as a diagnostic tool for sub clinical mastitis in ewes. Acta. Sci. Vet., 40: 1038.
20. Mavrogenis, A.P., Koimas, A., Kakoyiannis, C.K. and Taliotis, C.H. (1995) Use of somatic cell counts for the detection of subclinical mastitis in sheep. Small Ruminant. Res., 17: 79-84. [Crossref]