Aim: This study was conducted to demonstrate the acaricide efficacy of novel indigenous veterinary medication shared by an outstanding knowledge holder against naturally infested cattle and efforts in mainstreaming such wisdom.

Materials and Methods: An indigenous herbal medication in control of tick infestation was documented, and experimentation was held against naturally affected cattle. Eighteen clinically infested cattle population comprising 16 crossbred and 2 non-descript cattle were purposively selected. Majority of them were adult females, reported with a higher incidence of tick at Veterinary institution. The average pre-treatment tick count at 24 sites of observations among these animals was 18.91±2.04 (Mean [x̄]±standard error [SE]). The medication was topically applied once daily for 2 days and post-treatment observations were recorded for an experimental period of 14 days' duration.

Results: During 24-h post-treatment observation, the medication had shown 92.95% acaricidal property with clinically irrelevant rate of tick infestation of 1.33±0.39 (x̄±SE) was noticed before application of subsequent (second) dosage. This practice was found significantly effective at 5% level of significance (t_{0.05, 23}=9.08) illustrating faster relief to livestock. Animals were treated with herbal medication as per dosage on the second day and no reinfestation was noticed up to 14 days of experimental observation.

Conclusion: The study strengthens the belief that indigenous herbal acaricide can facilitate quality livestock service at geographically distant locations. These medications can provide quicker relief, minimize tick resistance and are favorable to the environment.

Keywords: acaricide, indigenous, institution, livestock, ruminant, tick.

1. Sarani, M., Telmadarraiy, Z., Moghaddam, A.S., Azam, K. and Sedaghat, M.M. (2014) Distribution of ticks (Acari: Ixodidae) infesting domestic ruminants in mountainous areas of Golestan province, Iran. Asian Pacific J. Trop. Biomed., 4: S246-S251. [Crossref]

2. Thakur, D., Sharma, A.K., Katoch, S., Chander, M., Mane, B.G. and Sharma, P. (2012) High altitude livestock farming: A participatory appraisal in Himachal Pradesh, India. Indian J. Anim. Sci., 82: 644-648.

3. Mondal, D.B., Sarma, K. and Saravanan, M. (2013) Upcoming of the integrated tick control program of ruminants with special emphasis on livestock farming system in India. Ticks Tick Borne Dis., 4: 1-10. [Crossref] [PubMed]

4. Wanzala, W., Takken, W., Mukabana, W.R., Pala, A.O., Hassanali, A. (2012) Ethnoknowledge of Bukusu community on livestock tick prevention and controlin Bungoma district, western Kenya. J. Ethnopharmacol., 140: 298-324. [Crossref] [PubMed]

5. Domingues, L.N., Brasil, B.S.A.F., Bello, A.C.P.P., da Cunha, A.P., de Barros, A.Y.M., Leite, R.C., Silaghi, C., Pfister, K. and Passos, L.M.F. (2012) Survey of pyrethroid and organophosphate resistance in Brazilian field populations of Rhipicephalus (Boophilus) Microplus: Detection of C190A mutation in domain II of the para-type sodium channel gene. Vet. Parasitol., 189: 327-332. [Crossref]

6. Velusamy, R., Rani, N., Ponnudurai, G., Harikrishnan, T.J., Anna, T., Arunachalam, K., Senthilvel, K. and Anbarasi, P. (2014) Influence of season, age and breed on prevalence of haemoprotozoan diseases in cattle of Tamil Nadu, India. Vet. World., 7: 574-578. [Crossref]

7. Asmaa, N.M., ElBably, M.A. and Shokier, K.A. (2014) Studies on prevalence, risk indicators and control options for tick infestation in ruminants. Beni Suef Univ. J. Basic Appl. Sci., 3: 68-73. [Crossref]

8. Beugnet, F., Liebbenberg, J. and Halos, L. (2015) Comparative speed of efficacy against Ctenocephalides felis of two oral treatments for dogs containing either afoxolanerorfluralaner. Vet. Parasitol., 207, 297-301. [Crossref] [PubMed]

9. Adenubi, O.T., Fasina, F.O., McGaw, L.J., Eloff, J.N. and Naidoo, V. (2016) Plant extracts to control ticks of veterinary and medical importance: A review. South Afr. J. Bot., 105: 178-193. [Crossref]

10. Byaruhanga, C., Oosthuizen, M.C., Collins, N.E. and Knobel, D. (2015) Using participatory epidemiology to investigate management options and relative importance of tick-borne diseases amongst transhumant zebu cattle in Karamoja Region, Uganda. Prev. Vet. Med., 122: 287-297. [Crossref]

11. Dantas-Torres, F. (2015) Climate change, biodiversity, ticks and tick-borne diseases: The butterfly effect. Int. J. Parasitol. Parasites Wildl., 4: 452-461. [Crossref] [PubMed] [PMC]

12. Wunder, S., Angelsen, A. and Belcher, B. (2014) Forests, livelihoods, and conservation: Broadening the empirical base. World Dev., 64: S1-S11. [Crossref]

13. Ghorai, S., Ghorai, N., Dutta, L., Bera, A., Ghorui, S., Kinhekar, A.S., Ingle, V.C., Sonkusale, P., Awandkar, S.P., Tembhurne, P.A., Kumar, V., Ravikumar, R.K. and Kumar, V. (2016) Protective and immuno-modulatory effect of low cost locally available technology from west Bengal, India under indigenous knowledge research system [IKRS]. J. Immunol. Immunopathol., 18: 19-23. [Crossref]

14. Ustyuzhantseva, O.V. (2015) Institutionalization of grassroots innovation in India. Curr. Sci., 108: 1476-1482.

15. Feola, G. (2017) Adaptive institutions? Peasant institutions and natural models facingclimatic and economic changes in the Colombian Andes. J. Rural Stud., 49: 117-127. [Crossref]

16. Gupta, A.K. (2012) Innovations for the poor by the poor. Int. J. Tech. Learn. Innov. Dev., 5: 28-39. [Crossref]

17. Van Veen, S.T.W. (1997) Sense or nonsense? Traditional methods of animal parasitic disease control. Vet. Parasitol., 71: 177-194. [Crossref]

18. Dhamale, M., Mahajan, A., Kinhekar, A.S., Rajurkar, G., Ravikumar, R.K., Ksheersagar, V.H. and Kumar, V. (2016) Reviving technology demonstration in farmer's field-An Appraisal. J. Exp. Biol. Agricul. Sci., 4: 39-47. [Crossref]

19. McCorkle, C.M. (1995), Back to the future: Lessons from ethnoveterinary RD and E for studying and applying local knowledge. Agric. Hum. Values, 12: 52-80. [Crossref]

20. Gupta, A.K., Sinha, R., Koradia, D., Patel, R., Parmar, M., Rohit, P., Patel, H., Patel, K., Chand, V.S., James, T.J., Chandan, A., Patel, M., Prakash, T.N., and Vivekanandan, P. (2003) Mobilizing grassroots' technological innovations and traditional knowledge, values and institutions: Articulating social and ethical capital. Futures, 35: 975-987. [Crossref]

21. Raot, U.S., Parmar, M., Patel, P., Patel, R., Gogoi, D.M., Patel, J., Sivapregasam, V., Kumar, V., Ravikumar, R.K. and Kumar, V. (2016) Value addition of novel herbal livestock medication Mastiherbin treatment of mastitis sustained by creative communities from the regions of Dang, Gujarat. Int. J. Bioresour. Stress Manag., 7: 501-507.

22. Mesfin, T., Obsa, T. (1994) Ethiopian traditional veterinary practices and their possible contribution to animal production and management. Rev. Sci. Tech. Off. Int. Epiz., 13: 417-424. [Crossref]

23. Asiedu, F., Gouro, A.S., Ndlovu, L., Nuru, H., Lameta, K. and Francis, J. (2009) Improving Livestock Development in ACP countries: The role of Science, Technology and Innovation in Addressing the Challenges to Food Security and Economic Empowerment. ACP policy brief No 1/2009, The ACP-EU Technical Centre for Agricultural and Rural Cooperation (CTA). Wageningen, Netherlands. Available from: http://www.src-jamaica.org/documents/LivestockPolicyBriefFinal_090529.pdf. Last accessed on 18.6.2017.

24. Rastogi, S., Pandey, M.K., Prakash, J., Sharma, A. and Singh, G.N. (2015) Veterinary herbal medicines in India. Pharm. Rev., 9: 155-163. [Crossref] [PubMed] [PMC]

25. Thomas, D., Zerbini, E., Rao, P.P. and Vaidyanathan, A. (2002) Increasing animal productivity on small mixed farms in South Asia: A systems perspective. Agric. Syst., 71: 41-57. [Crossref]

26. Thakur, D., Sharma, A.K., Ravikumar, R.K., Kumar, V. (2016) Benefit to end users: Appraisal of extending technology at farm fields from regions of Himachal Pradesh, India. J. Exp. Biol. Agric. Sci., 4: 1-8. [Crossref]

27. Giday, K., Lenaerts, L., Gebrehiwot, K., Yirga, G. and Muys, B. (2016) Ethnobotanical study of medicinal plants from degraded dry afromontane forest in northern Ethiopia: Species, uses and conservation challenges. J. Herbal Med., 6: 96-104. [Crossref]

28. TAP. (2016) Common Framework on Capacity Development for Agricultural Innovation Systems: Synthesis Document. Tropical Agriculture Platform (Tap), CAB International, Wallingford, UK. Available from: http://www.cabi.org/Uploads/CABI/about-us/4.8.5-other-business-policies-and-strategies/tap-synthesis-document.pdf. Last accessed on 18.6.2017.

29. Choudhary, H., Ravikumar R.K. and Kumar, V. (2016) Assessing behaviour of farmers in linking to village institution: Dairy society's perspective from semiarid regions, Gujarat, India. J. Exp. Biol. Agric. Sci., 4: 9-16. [Crossref]

30. Ravikumar, R.K., Dutta, L., Bera, A. and Kumar, V. (2016) Livestock Service through knowledge of society: Mainstreaming contribution of women knowledge holders. Int. J. Bioresour. Stress Manag., 7: 1168-1172.

31. CTA. (2015) CTA Top 20 Innovations That Benefit Smallholder Farmers. The ACP-EU Technical Centre for Agricultural and Rural Cooperation (CTA), Wageningen, The Netherlands. p1-73. Available from: http://www.knowledge.cta.int/content/download/1831_PDF_LQ.pdf. Last accessed on 18.6.2017.

32. Ravikumar, R.K., Periyaveeturaman, C., Selvaraju, D., Kinhekar, A.S., Dutta, L., Kumar, V. (2016a) Community oriented ectoparasite intervention system: Concepts for on-farm application of indigenous veterinary medication. Adv. Anim. Vet. Sci., 4: 9-19. [Crossref]

33. Ravikumar, R.K., Kumar, V., Choudhary, H., Kinhekar, A.S. and Kumar, V. (2015) Efficacy of indigenous polyherbal ectoparasiticide formulation against hard tick infestation in cattle (Bosindicus). Rumin. Sci., 4: 43-47.

34. Muraleedharan, K. and Sahadev, A. (2012) Possibility of harnessing the ectoparasiticidal potential of an ayurvedic liniment in young goats. Curr. Sci., 103: 11.

35. Gupta, S.P. (2000) Statistical Methods. Sultan Chandand Sons Educational Publishers, New Delhi.

36. Benelli, G. (2016) Tools to fight ticks: A never-ending story? News from the front of green acaricides and photosensitizers. Asian Pac. J. Trop. Dis., 6: 656-659. [Crossref]

37. Holdsworth, P.A., Kemp, D., Green, P., Peter, R.J., De Bruin, C., Jonsson, N.N., Letonja, T., Rehbein, S. and Vercruysse, J. (2006) World Association for the advancement of veterinary parasitology (W.A.A.V.P.) guidelines for evaluating the efficacy of acaricides against ticks (Ixodidae) on ruminants. Vet. Parasitol., 136: 29-43. [Crossref]

38. Chander, M., Dutt, T., Ravikumar, R.K. and Subrahmanyeswari, B. (2010) Livestock technology transfer service in India: A review. Indian J. Anim. Sci., 80: 1115-1125.

39. Ravikumar, R.K. and Chander, M. (2006) Extension educational efforts by state department of animal husbandry (SDAH), Tamil Nadu: SWOT analysis. Livestock Res. Rural Dev., 18: 757-762.

40. Pavela, R., Canale, A., Mehlhorn, H. and Benelli, G. (2016) Application of ethnobotanical repellents and acaricides in prevention, control and management of livestock ticks: A review. Res. Vet. Sci., 109: 1-9. [Crossref]

41. Sheng-Ji, P. (2001) Ethnobotanical approaches of traditional medicine studies: Some experiences from Asia. Pharm. Biol., 39: 74-79. [PubMed]

42. Cochard, R. and Dar, M.E.U.I. (2014) Mountain farmers' livelihoods and perceptions of forest resource degradation at Machiara National Park, Pakistan-administered Kashmir. Environ. Dev., 10: 84-103. [Crossref]

43. Ravikumar, R.K., Thakur, D., Choudhary, H., Kumar, V., Kinhekar, A.S., Garg, T., Ponnusamy, K., Bhojne, G.R., Shetty, V.M. and Kumar, V. (2017) Social engineering of societal knowledge in livestock science: Can we be more empathetic? Vet. World, 10: 86-91. [Crossref]

44. Kadivendi, M., Maheswari, R., Ravikumar, R.K., Chauhan, M.M., Kinhekar, A.S., Kumar, V. and Kumar, V. (2015) Integrated approach for engaging farming community - Opportunities and challenges for low cost inputs. Int. J. Agric. Innov. Res., 3: 1691-1695.

45. Mierzejewska, E.J., Alsarraf, M., Behnkem, J.M. and Bajer, A. (2015) The effect of changes in agricultural practices on the density of Dermacentor reticulatus ticks. Vet. Parasitol., 211: 259-265. [Crossref] [PubMed]

46. Chinsembu, K.C., Negumbo, J., Likando, M. and Mbangu, A. (2014) An ethnobotanical study of medicinal plants used to treat livestock diseases in Onayena and Katima Mulilo, Namibia. South Afr. J. Bot., 94: 101-107. [Crossref]

47. Wall, R., Rose, H., Ellse, L. and Morgan, E. (2011) Livestock ectoparasites: Integrated management in a changing climate. Vet. Parasitol., 180: 82-89. [Crossref] [PubMed]

48. Kioko, J., Baker, J., Shannon, A. and Kiffner, C. (2015) Ethnoecological knowledge of ticks and treatment of tick-borne diseases among Maasai people in Northern Tanzania. Vet. World, 8: 755-762. [Crossref] [PubMed] [PMC]

49. Rogers, E.M. (2003) Diffusion of Innovations. 5th ed. Free Press, New York. p365-401.

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