doi: 10.14202/vetworld.2018.706-711
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Article history: Received: 20-01-2018, Accepted: 25-04-2018, Published online: 27-05-2018
Corresponding author: Tippawan Jantafong
E-mail: jantafong1980@gmail.com
Citation: Jantafong T, Ruenphet S, Punyadarsaniya D, Takehara K (2018) The study of effect of didecyl dimethyl ammonium bromide on bacterial and viral decontamination for biosecurity in the animal farm, Veterinary World, 11(5): 706-711.Aim: The aim of this study was to determine the effectiveness of the fourth-generation quaternary ammonium compounds, didecyl dimethyl ammonium bromide (DDAB), on the efficacy of bacterial and viral decontamination against pathogens commonly found in livestock industry including Salmonella infantis (SI), Escherichia coli, and avian influenza virus (AIV).
Materials and Methods: The DDAB was prepared at 500, 250, and 125 parts per million (ppm) for absent and present organic material. Meanwhile, 5% of fetal bovine serum in DDAB solution sample was used to mimic the presence of organic material contamination. 400 μl of each DDAB concentration was mixed with 100 μl of each pathogen (SI, E. coli, and AIV) and then incubated at room temperature or 4°C at various time points (5 s, 30 s, 1 min, 5 min, 10 min, 15 min, and 30 min). The activity of DDAB treatment was stopped using 500 μl of FBS. Each treatment sample was titrated on either deoxycholate hydrogen sulfide lactose agar plates or Madin-Darby canine kidney cells for bacteria and AIV, respectively. Each treatment was conducted in triplicates, and the pathogen inactivation was considered effective when the reduction factor was ≥ 3 log10.
Results: Our current study revealed that the DDAB inactivated SI, E. coli, and AIV under the various concentrations of DDAB, organic material conditions, exposure temperature, and exposure timing. In addition, the comparison of bactericidal and virucidal efficacy indicated that bacteria were more susceptible to be inactivated by DDAB as compared to viruses. However, DDAB showed marked inactivated differences in the absence or presence of organic materials.
Conclusion: The DDAB may be a potential disinfectant for inactivating bacteria and viruses, especially enveloped viruses, in livestock farms. It can be useful as a disinfectant for biosecurity enhancement on and around animal farm.
Keywords: bactericidal, didecyl dimethyl ammonium bromide, disinfectant, quaternary ammonium compound, virucidal.
1. Meng, J., LeJeune, J., Zhao, T. and Doyle, M. (2013) Enterohemorrhagic Escherichia coli. In: Doyle, M., Buchanan, R., editor. Food Microbiology. ASM Press, Washington, DC. p287-309.
2. Dale, E. and Brown, C. (2013) Zoonotic diseases from poultry. Braz. J. Vet. Pathol., 6(2) : 76-82.
3. Nidaullah, H., Abirami, N., Shamila-Syuhada, A.K., Chuah, L.O., Nurul, H., Tan, T.P., Abidin, F.W.Z and Rusul, G. (2017) Prevalence of Salmonella in poultry processing environments in wet markets in Penang and Perlis, Malaysia. Vet. World, 10(3): 286-292. [Crossref] [PubMed] [PMC]
4. Giraudon, I., Cathcart, S., Blomqvist, S., Littleton, A., Surman-Lee, S., Mifsud, A., Anaraki, S. and Fraser, G. (2009). Large outbreak of salmonella phage type 1 infection with high infection rate and severe illness associated with fast food premises. Public Health, 123(6): 444-447. [Crossref] [PubMed]
5. Lekcharoensuk, P. (2008) Highly pathogenic avian influenza (HPAI) H5N1 Virus in Asia: Evolution and vaccination. Vet. World, 1(12): 368-374.
6. Hanson, R., Kaneene, J.B., Padungtod, P., Hirokawa, K. and Zeno, C. (2002) Prevalence of Salmonella and E. coli, and their resistance to antimicrobial agents, in farming communities in northern Thailand. Southeast Asian J. Trop. Med. Public Health., 33(3): 120-126. [PubMed]
7. Angkititrakul, S., Polpakdee, A. and Chuanchuen, R. (2013) Prevalence of Salmonella enterica, Escherichia coli and Staphylococcus aureus in raw meat in Thai self-service style restaurants in Khon kaen municipality. Thai J. Vet. Med., 43(2): 265-268.
8. Souris, M., Selenic, D., Khaklang, S., Ninphanomchai, S., Minet, G., Gonzalez, J.P. and Kittayapong, P. (2014) Poultry farm vulnerability and risk of avian influenza re-emergence in Thailand. Int. J. Environ. Res. Public Health., 11(1): 934-951. [Crossref] [PubMed] [PMC]
9. Gosling, R.J., Mawhinney, I., Vaughan, K., Davies, R.H. and Smith, R.P. (2017) Efficacy of disinfectants and detergents intended for a pig farm environment where Salmonella is present. Vet. Microbiol ., 204 : 46-53. [Crossref] [PubMed]
10. Zhang, C., Cui, F., Zeng, G., Jiang, Z., Yang, Z., Yu, G., Zhu, M. and Shen, L. (2015) Quaternary ammonium compounds (QACs): A review on occurrence, fate and toxicity in the environment. Sci. Total Environ., 518 : 352-362. [Crossref] [PubMed]
11. Ota, M., Toyofuku, C., Thammakarn, C., Sangsriratanakul, N., Yamada, M., Nakajima, K., Kitazawa, M., Hakim, H., Alam, M.S., Shoham, D. and Takehara, K. (2016) Calcinated egg shell as a candidate of biosecurity enhancement material. J. Vet. Med. Sci., 78(5): 831-836. [Crossref] [PubMed] [PMC]
12. Sonthipet, S., Ruenphet, S. and Takehara, K. (2018) Bactericidal and virucidal efficacies of potassium monopersulfate and its application for inactivating avian influenza virus on virus-spiked clothes. J. Vet. Med. Sci., 80(4): 568-573. [Crossref] [PubMed] [PMC]
13. Thammakarn, C., Satoh, K., Suguro, A., Hakim, H., Ruenphet, S. and Takehara, K. (2014) Inactivation of avian influenza virus, newcastle disease virus and goose parvovirus using solution of nano-sized scallop shell powder. J. Vet. Med. Sci., 76(9): 1277-1280. [Crossref] [PMC]
14. Lombardi, M.E., Ladman, B.S., Alphin, R.L. and Benson, E.R. (2008) Inactivation of avian influenza virus using common detergents and chemicals. Avian Dis., 52(1): 118-123. [Crossref] [PubMed]
15. Takehara, K., Yamazaki, K., Miyazaki, M., Yamada, Y., Ruenphet, S., Jahangir, A., Shoham, D., Okumura, M. and Nakamura, M. (2010) Inactivation of avian influenza virus H1N1 by photocatalyst under visible light irradiation. Virus Res., 151(1): 102-103. [Crossref] [PubMed]
16. Stringfellow, K., Anderson, P., Caldwell, D., Lee, J., Byrd, J., McReynolds, J., Carey, J., Nisbet, D. and Farnell, M. (2009) Evaluation of disinfectants commonly used by the commercial poultry industry under simulated field conditions. Poult. Sci., 88(6) : 1151-1155. [Crossref] [PubMed]
17. Jang, Y., Lee, K., Yun, S., Lee, M., Song, J., Chang, B. and Choe. N.H. (2017) Efficacy evaluation of commercial disinfectants by using Salmonella enterica serovar Typhimurium as a test organism. J. Vet. Sci., 18(2): 209-216. [Crossref] [PubMed] [PMC]
18. Hegstad, K., Langsrud, S., Lunestad, B.T., Scheie, A.A., Sunde, M. and Yazdankhah, S.P. (2010) Does the wide use of quaternary ammonium compounds enhance the selection and spread of antimicrobial resistance and thus threaten our health? Microb. Drug Resist., 16(2) : 91-104. [Crossref] [PubMed]
19. Rajkowska, K., Kozirog, A., Otlewska, A., Piotrowska, M., Nowicka-Krawczyk, P., Brycki, B., Kunicka-Styczynska, A. and Gutarowska, B. (2016) Quaternary ammonium biocides as antimicrobial agents protecting historical wood and brick. Acta Biochim. Pol., 63(1) : 153-159. [Crossref] [PubMed]
20. Martin, H., Le Potier, M.F. and Maris, P. (2007) Virucidal efficacy of nine commercial disinfectants against porcine circovirus type 2. Vet. J., 177: 388-393. [Crossref] [PubMed]
21. Yamanaka, T., Bannai, H., Tsujimura, K., Nemoto, M., Kondo, T. and Matsumura, T. (2014) Comparison of the virucidal effects of disinfectant agents against equine influenza a virus. J. Equine. Vet. Sci ., 34: 715-718. [Crossref]
22. Chaichoune, K., Wiriyarat, W., Thitithanyanont, A., Phonarknguen, R., Sariya, L., Suwanpakdee, S., Noimor, T., Chatsurachai, S., Suriyaphol, P., Ungchusak, K., Ratanakorn, P., Webster, R.G., Thompson, M., Auewarakul, P. and Puthavathana, P. (2009) Indigenous sources of 2007-2008 H5N1 avian influenza outbreaks in Thailand. J. Gen. Virol., 90(Pt 1) : 216-222. [Crossref] [PubMed]
23. Wanaratana, S., Tantilertcharoen, R., Sasipreeyajan, J. and Pakpinyo, S. (2010) The inactivation of avian influenza virus subtype H5N1 isolated from chickens in Thailand by chemical and physical treatments. Vet. Microbiol ., 140: 43-48. [Crossref] [PubMed]
24. Krangvichain, P., Niyomtham, W. and Prapasarahul, N. (2015) Occurrence and susceptibilities to disinfectants of Cryptococcus neoformans in fecal droppings from pigeons in Bangkok, Thailand. J. Vet. Med. Sci., 78(3): 391-396. [Crossref] [PubMed] [PMC]
25. Oliveira, T.M.L., Rehfeld, I.S., Coelho Guedes, M.I.M., Ferreira, J.M.S., Kroon, E.G. and Lobato, Z.I.P. (2011) Susceptibility of vaccinia virus to chemical disinfectants. Am. J. Trop. Med. Hyg., 85(1): 152-157. [Crossref] [PubMed] [PMC]