Background and Aim: Toxoplasmosis is a worldwide zoonotic disease with harmful effects on animal and human health. Ingestion of contaminated raw milk has been suggested as a vehicle for transmission of Toxoplasma gondii to human. The present study was performed for the detection of T. gondii in raw milk samples of goat, sheep, and camel in Upper Egypt using two different techniques (enzyme-linked immunosorbent assay [ELISA] and quantitative polymerase chain reaction [qPCR]).

Materials and Methods: This study was conducted to determine the T. gondii prevalence using ELISA and qPCR in raw goat, sheep, and camels milk (30 samples for each) collected from different locations in Upper Egypt.

Results: T. gondii IgG antibodies were detected in 90.0, 60.0, and 3.33% of goat, sheep, and camel milk samples, respectively. From the positive samples of T. gondii IgG, the parasitic DNA was detected only in two examined milk samples, one of them was present in goat milk sample and another one was found in sheep milk sample. On the other hand, the parasite was not detected in camels' milk samples.

Conclusion: These results concluded that the raw milk was contaminated by T. gondii tachyzoites which could be a source of human infection. Restricted hygienic programs should be implemented in the animal farms to decrease the risk of milk contamination by this parasite.

Keywords: camel milk, enzyme-linked immunosorbent assay, goat milk, quantitative polymerase chain reaction, sheep milk, Toxoplasma gondii.

1. CDC Center for Disease Control and Prevention. Parasites toxoplasmosis (Toxoplasma infection) (2013) Available from: http://www.cdc.gov/parasites/toxoplasmosis/index.html. Last accessed on 20-07-2018.

2. Montoya, J.G. and Liesenfeld, O. (2004) Toxoplasmosis. Lancet, 363: 1965-1976. [Crossref]

3. Mancianti, F., Nardoni, S., D'Ascenzi, C., Pedonese, F., Mugnaini, L., Filomena, F. and Papini, R. (2013) Seroprevalence, detection of DNA in blood and milk, and genotyping of Toxoplasma gondii in a goat population in Italy. BioMed Res. Int., 2013: Article ID: 905326, 6.

4. Tenter, A.M., Heckeroth, A.R. and Weiss, L.M. (2000) Toxoplasma gondii: From animals to humans. Int. J. Parasitol., 30: 1217-1258. [PubMed]

5. Kamani, J., Mani, A.U. and Egwu, G.O. (2010) Seroprevalence of Toxoplasma gondii infection in domestic sheep and goats in Borno state, Nigeria. Trop Anim. Health Prod., 42: 793-797. [Crossref] [PubMed]

6. Reisch, U., Bretagne, S., Kruger, D., Ernault, P. and Costa, M. (2003) Comparison of two DNA targets for the diagnosis of toxoplasmosis by real-time PCR using fluorescence resonance energy transfer hybridization probes. BMC Infect Dis., 3: 7. [Crossref] [PMC]

7. Dubey, J.P. (1998) Comparative infectivity of Toxoplasma gondii bradyzoites in rats and mice. J. Parasitol., 84: 1279-1282. [Crossref] [PubMed]

8. Dehkordi, F.S., Rahimi, E. and Abdizadeh R. (2013) Detection of Toxoplasma gondii in raw Caprine, Ovine, Buffalo, Bovine, and Camel Milk Using Cell cultivation, cat bioassay, capture ELISA, and PCR methods in Iran. Foodborne Pathog. Dis., 10(2): 120-125. [Crossref] [PubMed]

9. Medani, M.Y. and Mohamed, H. (2016) Camel's milk as a source of human toxoplasmosis in Butana area-Sudan. Int. J. Inf. Dis., 45 Supp 1: 471. [Crossref]

10. Camossi, L.G., Greca-Junior, H., Correa, A.P., Richini-Pereira, V.B., Silva, R.C., Da Silva, A.V. and Langoni, H. (2011) Detection of Toxoplasma gondii DNA in the milk of naturally infected ewes. Vet. Parasitol., 177: 256-261. [Crossref] [PubMed]

11. Switaj, L., Master, A., Skrzypczak, M. and Zaborowski, P. (2005) Recent trends in molecular diagnostics for Toxoplasma gondii infections. Clin. Microbiol. Infect., 11: 170-176. [Crossref] [PubMed]

12. Martins, T.B., Hillyard, D.R., Litwin, C.M., Taggart, E.W., Jaskowski, T.D. and Hill, H.R. (2000) Evaluation of a PCR probe capture assay for the detection of Toxoplasma gondii. Am. J. Clin. Pathol., 113: 714-721. [Crossref] [PubMed]

13. Held, T.K., Kruger, D., Switala, A.R., Beyer, J., Kingreen, D., Busemann, C., Janitschke, K. and Siegert, W. (2000) Diagnosis of toxoplasmosis in bone marrow transplant recipients: Comparison of PCR based results and immunohistochemistry. Bone Marrow Transplant, 25: 1257-1262. [Crossref] [PubMed]

14. Kompalic-Cristo, A., Frotta, C., Sua'rez-Mutis, M., Fernandes, O. and Britto, C. (2007) Evaluation of a real-time PCR assay based on the repetitive B1 gene for the detection of Toxoplasma gondii in human peripheral blood. Parasitol. Res., 101: 619-625. [Crossref] [PubMed]

15. Cook, A.J.C., Gilbert, R.E., Buffolano, W., Zufferey, J., Peterse, E., Jenum, P.A., Foulon, W., Semprini, A.E. and Dunn, D.T. (2000) Sources of Toxoplasma infection in pregnant women: European multicentre case-control study. BMJ, 321: 142-147. [Crossref] [PubMed] [PMC]

16. Hiramoto, R.M., Mayrbaurl-Borges, M., Galisteo, A.J., Meireles, L.R., Macre, M.S. and Andrade, H.F. (2001) Infectivity of cysts of the ME-49 Toxoplasma gondii strain in bovine milk and homemade cheese. Rev. Saude Publ., 35: 113-118. [Crossref]

17. Abdel-Rahman, M.A.M., EL-Manyawe, S.M., Khateib, A.M. and Saba, S. (2012) Occurrence of Toxoplasma antibodies in caprine milk and serum in Egypt. Assiut Vet. Med. J., 58(133): 145-152.

18. Sadek, O.A., Abdel-Hameed, Z.M. and Kuraa, H.M. (2015) Molecular detection of Toxoplasma gondii DNA in raw goat and sheep milk with discussion of its public health importance in Assiut Governorate. Assiut Vet. Med. J., 61(145): 166-177.

19. Mosa, A.I. (2016) Occurrence of Cryptosporidium Species and Toxoplasma gondii as Protozoan Parasites in Raw Milk. Ph. D. Degree. Faculty of Veterinary Medicine, Assiut University, Egypt.

20. Dubey, J.P. (2004) Toxoplasmosis waterborne zoonosis. Vet. Parasitol., 163: 1-14. [Crossref] [PubMed]

21. Jittapalapong, S., Sangvaranond, A., Inpankaew, T., Phasuk, C., Pinyopanuwat, N., Chimnoi, W. Kengradomkij, C., Arunvipat, P. and Maruyama, S. (2008) Seroprevalence of Toxoplasma gondii infections of dairy cows in Northeastern Thailand. South East Asian J. Trop. Med. Public Health, 39(1): 1-5.

22. Ossani, R.A., Broges, H.A., Souza, A.P., Sartor, A., Miletti, L.C., Federle, M. and Moura, A.B. (2017) Toxoplasma gondii in milk naturally infected dairy ewes on west mesoregion of Santa Catrina state, Brazil. Arg. Bras. Med. Vet. Zoontec., 69(5): 1294-1300. [Crossref]

23. Amairia, S., Rouatbi, M., Rjeibi, M.R., Nouasri, H., Sassi, L., Mhadhbi, M. and Gharbi, M. (2016) Molecular prevalence of Toxoplasma gondii DNA in goats' milk and seroprevalence in Northwest Tunisia. Vet. Med. Sci., 2: 154-160. [Crossref] [PubMed] [PMC]

24. Tavassoli, M., Esmaeilnejad, B., Malekifard, F., Soleimanzadeh, A. and Dilmaghani, M. (2013) Detection of Toxoplasma gondii DNA in sheep and goat milk in Northwest of Iran by PCR-RFLP. Jundishapur J. Microbiol., 6(10): e8201. [Crossref]

25. Luptakova, L., Petrovova, E. and Stavova, L. (2013) Diagnostics of Toxoplasma gondii in milk samples of sheep from Eastern Slovakia. Anim. Welf. Ethol. Hous. Syst., 9(3): 567-569.

26. Vismarra, A., .Barilli, E., Miceli, M., Mangia, C., Bacci, C., Brindani, F. and Kramer, L. (2017) Toxoplasma gondii and pre-treatment protocols for polymerase chain reaction analysis of milk samples: A field trial in sheep from Southern Italy. J. Food Saf., 6: 6501.

27. Hierl, T., Reischl, U., Peter, L.P., Holger, H.H., Stark, M., Pierre, K.P and Autenrieth, I.B. (2004) Preliminary evaluation of one conventional nested and two real-time PCR assays for the detection of Toxoplasma gondii in immunocompromised patients. J. Med. Microbiol., 53: 629-632. [Crossref] [PubMed]

28. Robert-Gangneux, F., Brenier-Pinchart, M., Year, H., Belaz, S., Varlet-Marie, E. and Patrick Bastien, P. (2017) Evaluation of Toxoplasma ELITe MGB real-time PCR assay for diagnosis of toxoplasmosis. J. Clin. Micribiol., 55(5): 1369-1376. [Crossref] [PubMed] [PMC]

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