Open Access
Research (Published online: 23-08-2019)
18. Effect of the pH in the enrichment of X or Y sex chromosome-bearing sperm in bovine
Nidhi P. Raval, Tejas M. Shah, Linz-Buoy George and Chaitanya G. Joshi
Veterinary World, 12(8): 1299-1303

Nidhi P. Raval: Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India; Department of Zoology, Biomedical Technology and Human Genetics, University School of Sciences, Gujarat University, Ahmedabad, Gujarat, India.
Tejas M. Shah: Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India.
Linz-Buoy George: Department of Zoology, Biomedical Technology and Human Genetics, University School of Sciences, Gujarat University, Ahmedabad, Gujarat, India.
Chaitanya G. Joshi: Gujarat Biotechnology Research Centre, Block B and D, 6th Floor, MS Building, Sector - 11, Gandhinagar, Gujarat, India.

doi: 10.14202/vetworld.2019.1299-1303

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Article history: Received: 27-04-2019, Accepted: 05-07-2019, Published online: 23-08-2019

Corresponding author: Chaitanya G. Joshi

E-mail: dir-gbrc@gujarat.gov.in

Citation: Raval NP, Shah TM, George LB, Joshi CG (2019) Effect of the pH in the enrichment of X or Y sex chromosome-bearing sperm in bovine, Veterinary World, 12(8): 1299-1303.
Abstract

Background and Aim: Studies have shown that the pH of the vagina during the course of fertilization may influence the migration of X- and Y-bearing spermatozoa and thus leading to skewness in the sex of the offspring. Hence, this study was carried out to check the effect of the pH in the enrichment of X or Y sex chromosome-bearing sperm in bovine (Bos indicus).

Materials and Methods: To check the effect of pH in the enrichment of X or Y sex chromosome-bearing sperm in bovine, we used buffers of various pH ranging from 5.5 to 9.0 for swim-up procedure of sperm sample and collected upper and bottom fraction from the same buffer and checked the abundance of X- and Y-bearing spermatozoa by droplet digital polymerase chain reaction using X- and Y-chromosome-specific DNA probe.

Results: The abundance of X- and Y-bearing spermatozoa was not differed significantly in either of the fraction collected.

Conclusion: Thus, it appears to be unlikely that an immediate impact of pH on sperm can be a solitary impact on the sex of offspring in bovine.

Keywords: droplet digital polymerase chain reaction, spermatozoa, swim-up.