Open Access
Research (Published online: 17-12-2019)
15. Hepatoprotective effect of crude polysaccharides extracted from Ganoderma lucidum against carbon tetrachloride-induced liver injury in mice
Raden Joko Kuncoroningrat Susilo, Dwi Winarni, Saikhu Akhmad Husen, Suhailah Hayaza, Hunsa Punnapayak, Sri Puji Astuti Wahyuningsih, Elma Sakinatus Sajidah and Win Darmanto
Veterinary World, 12(12): 1987-1991

Raden Joko Kuncoroningrat Susilo: Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia.
Dwi Winarni: Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia.
Saikhu Akhmad Husen: Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia.
Suhailah Hayaza: Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia.
Hunsa Punnapayak: Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia; Plant Biomass Utilization Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Sri Puji Astuti Wahyuningsih: Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia.
Elma Sakinatus Sajidah: Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia.
Win Darmanto: Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia.

doi: www.doi.org/10.14202/vetworld.2019.1987-1991

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Article history: Received: 01-08-2019, Accepted: 08-11-2019, Published online: 17-12-2019

Corresponding author: Win Darmanto

E-mail: windarmanto@fst.unair.ac.id

Citation: Susilo RJK, Winarni D, Husen SA, Hayaza S, Punnapayak H, Wahyuningsih SPA, Sajidah ES, Darmanto W (2019) Hepatoprotective effect of crude polysaccharides extracted from Ganoderma lucidum against carbon tetrachloride-induced liver injury in mice, Veterinary World, 12(12):1987-1991.
Abstract

Background and Aim: Natural products are currently widely used as alternative treatments for liver disease. The study aimed to determine the hepatoprotective effect of crude polysaccharides extracted from Ganoderma lucidum against liver injury induced by carbon tetrachloride (CCl4).

Materials and Methods: Twenty-four male BALB/C mice were randomly divided into six groups. Serum and liver samples were taken on day 10 after G. lucidum administration. The levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) were measured using enzyme-linked immunosorbent assays, and the histology of the liver was evaluated using light microscopy.

Results: G. lucidum extract significantly decreased the levels of ALT, AST, and MDA and significantly increased the levels of SOD and CAT. In the histological evaluation, the liver tissue of CCl4-treated mice exhibited hydropic degeneration, necrosis, and sinusoidal dilatation. G. lucidum extract administration improved this liver tissue histopathology.

Conclusion: Crude polysaccharides extracted from G. lucidum showed a hepatoprotective effect, regenerating damaged liver tissue.

Keywords: carbon tetrachloride, crude polysaccharide extract, Ganoderma lucidum, hepatotoxicity.