Pasteurellaceae members with similar morphological patterns associated with respiratory manifestations in ducks

Research (Published online: 26-12-2019)

24. Pasteurellaceae members with similar morphological patterns associated with respiratory manifestations in ducks

Samah Eid, Sherif Marouf, Hefny Y. Hefny and Nayera M. Al-Atfeehy

Veterinary World, 12(12): 2061-2069

Samah Eid: Department of Bacteriology, Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agricultural Research Centre, Nadi El-Seid St., P.O. Box 246, Dokki, Giza 12618, Egypt.

Sherif Marouf: Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.

Hefny Y. Hefny: Department of Poultry Diseases, Zagazig Provincial Laboratory, Animal Health Research Institute, Agricultural Research Centre, Sharkia, Egypt.

Nayera M. Al-Atfeehy: Department of Bacteriology, Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agricultural Research Centre, Nadi El-Seid St., P.O. Box 246, Dokki, Giza 12618, Egypt.

doi: www.doi.org/10.14202/vetworld.2019.2061-2069

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Article history: Received: 30-08-2019, Accepted: 25-11-2019, Published online: 26-12-2019

Corresponding author: Samah Eid

E-mail: samaheid@ymail.com

Citation: Eid S, Marouf S, Hefny HY, Al-Atfeehy NM (2019) Pasteurellaceae members with similar morphological patterns associated with respiratory manifestations in ducks, Veterinary World, 12(12): 2061-2069.

Abstract

Aim: A total of 112 freshly dead ducks aged from 2 to 20 weeks old with a history of respiratory manifestations were investigated for the implication of Pasteurellaceae family members.

Materials and Methods: Isolation and identification to the family level were conducted by conventional bacteriological methods, including microscopic examination and biochemical characterization. Identification to the species level was conducted by polymerase chain reaction (PCR) and analytical profile index (API) 20E kits.

Results: Conventional bacteriological isolation and biochemical characterization revealed the infection of 16/112 examined birds with a prevalence rate of 14.3%. PCR confirmed the detection of Pasteurellaceae family conserved genes RpoB and Bootz in 16/16 (100%) isolates. PCR was also used for genus and species identification of the isolated Pasteurellaceae members; the results revealed that 5/16 (31.3%) of isolates were Gallibacterium anatis and 2/16 of isolates (12.5%) were Pasteurella multocida. Riemerella anatipestifer, Mannheimia haemolytica, and Avibacterium paragallinarum were not detected by PCR. Biotyping by API 20E successfully identified 5/16 (31.3%) isolates that could not be typed by PCR and confirmed their belonging to Pasteurella pneumotropica. Neither the available PCR primer sets nor API 20E succeeded for species identification of 4/16 (25%) isolates. Antibiotic susceptibility profiling of isolates revealed that 16/16 (100%) of isolates demonstrated multidrug resistance (MDR) phenotypes. Moreover, 16/16 (100%) of isolates demonstrated a phenotypic resistance pattern to neomycin.

Conclusion: Combined genotypic, phenotypic, biotyping, and virulence characterizations are required for laboratory identification of pathogenic Pasteurellaceae. Moreover, P. multocida was not the prevailed member implicated in respiratory problems in ducks as P. pneumotropica, G. anatis, and unidentified strains were involved with higher prevalence. Chloramphenicol and ampicillin demonstrated the highest in vitro effects on the studied Pasteurellaceae. Furthermore, the prevalence of multidrug-resistant isolates signified the demand to implement targeted surveillance in the ducks' production sector, and MDR survey in poultry sectors in Egypt to apply effective control measures.

Keywords: ducks, Gallibacterium anatis, Mannheimia haemolytica, Pasteurella multocida, Riemerella anatipestifer.