Background and Aim: Acetamiprid is a chemical of neonicotinoid group which binds with nicotinic acetylcholine receptor (nAChR) and alters the brain function. The present study was taken up to enlight the understanding of nociception behavior in Sprague Dawley (SD) rat after multiple exposures to acetamiprid.

Materials and Methods: For experiment purpose, a total of 48 SD rats were divided into four dose groups having 12 animals each. Group I was control group received only distilled water. Group II, Group III, and Group IV were treated with acetamiprid at a dose rate of 5, 20, and 40 mg/kg body weight, respectively. Rats were tested in induced pain by formalin injection and tail flick test.

Results: The flinch counts in formalin-induced pain in acetamiprid-treated rat were reduced in a dose-dependent manner, whereas, in tail flick test, no such altered pain behavior was observed in treated group compared to control animals.

Conclusion: Acetamiprid alters the centralized nociception through nAChR but could not trigger the associated signal to inhibit the nociception peripherally.

Keywords: acetamiprid, formalin, pain, tail flick.

1. Sharifzadeh, M.S., Abdollahzadeh, G., Damalas, C.A. and Rezaei, R. (2018) Farmers' criteria for pesticide selection and use in the pest control process. Agriculture, 8(2): 24. [Crossref]

2. Yanga, G., Lib, J., Wanga, Y., Chenc, C., Zhaoa, H. and Shaod, K. (2018) Quantitative ecotoxicity analysis for pesticide mixtures using benchmark dose methodology. Ecotoxicol. Environ. Saf., 159: 94-101. [Crossref] [PubMed]

3. Honda, H., Tomizawa, M. and Casida, J.E. (2006) Insect nicotinic acetylcholine receptors:? Neonicotinoid binding site specificity is usually but not always conserved with varied substituents and species. J. Agric. Food Chem., 54(9): 3365-3371. [Crossref] [PubMed]

4. Dani, J.A. (2015) Neuronal nicotinic acetylcholine receptor structure and function and response to nicotine. Int. Rev. Neurobiol., 124: 3-19. [Crossref] [PubMed] [PMC]

5. Mondal, S., Sengupta, T., Pradhan, S., Hansda, R.N., Mandal, P.S., Tiwari, R. and Mukhopadhayay, S.K. (2014) Impaired learning and memory after a week-long exposure of acetamiprid in adult rats. Adv. Anim. Vet. Sci., 2(10): 543-548. [Crossref]

6. Cheng, L.Z., Han, L., Fan, J., Huang, L.T., Peng, L.C. and Wang, Y. (2011) Enhanced inhibitory synaptic transmission in the spinal dorsal horn mediates antinociceptive effects of TC-2559. Mol. Pain, 7: 56-65. [Crossref] [PubMed] [PMC]

7. Shi, Y., Weingarten, T.N., Mantilla, C.B., Hooten, W.M. and Warner, D.O. (2010) Smoking and pain pathophysiology and clinical implications. Anesthesiology, 113(4): 977-992. [Crossref] [PubMed]

8. Decker, M.W., Meyer, M.D. and Sullivan, J.P. (2001) The therapeutic potential of nicotinic acetylcholine receptor agonists for pain control. Expert. Opin. Investig. Drugs, 10(10): 1819-1830. [Crossref] [PubMed]

9. Corringer, P.J., Le, N.N. and Changeux, J.P. (2000) Nicotinic receptors at the amino acid level. Annu. Rev. Pharmacol. Toxicol., 40(1): 431-458. [Crossref] [PubMed]

10. Mattila, M.J., Ahtee, L. and Saarnivaara, L. (1968) The analgesic and sedative effects of nicotine in white mice, rabbits and golden hamsters. Ann. Med. Exp. Biol. Fenn., 46(1): 78-84. [PubMed]

11. Iwamoto, E.T. and Marion, L. (1993). Adrenergic, serotonergic and cholinergic components of nicotinic antinociception in rats. J. Pharmacol. Exp. Ther., 265(2): 777-789. [PubMed]

12. Iwamoto, E.T. (1991) Characterization of the antinociception induced by nicotine in the pedunculopontine tegmental nucleus and the nucleus raphe magnus. J. Pharmacol. Exp. Ther., 257(1): 120-133. [PubMed]

13. Iwamoto, E.T. (1989) Antinociception after nicotine administration into the mesopontinetegmentum of rats: Evidence for muscarinic actions. J. Pharmacol. Exp. Ther., 251(2): 412-421. [PubMed]

14. Damaj, M.I., Fei-Yin, M., Dukat, M., Glassco, W., Glennon, R.A. and Martin, B.R. (1998) Antinociceptive responses to nicotinic acetylcholine receptor ligands after systemic and intrathecal administration in mice. J. Pharmacol. Exp. Ther., 284(3): 1058-1065. [PubMed]

15. Cucchiaro, G., Chaijale, N. and Commons, K.G. (2005) The dorsal raphe nucleus as a site of action of the antinociceptive and behavioral effects of the a4 nicotinic receptor agonist epibatidine. J. Pharmacol. Exp. Ther., 313(1): 389-394. [Crossref] [PubMed]

16. Mao, D., Yasuda, R.P., Fan, H., Wolfe, B.B. and Kellar, K.J. (2006) Heterogeneity of nicotinic cholinergic receptors in rat superior cervical and nodose ganglia. Mol. Pharmacol., 70(5): 1693-1699. [Crossref] [PubMed]

17. Turner, J.R. and Kellar, K.J. (2005) Nicotinic cholinergic receptors in the rat cerebellum: Multiple heteromeric subtypes. J. Neurosci., 25(40): 9258-9265. [Crossref] [PubMed]

18. Damaj, M.I., Fonck, C., Marks, M.J., Deshpande, P., Labarca, C., Lester, H.A., Collins, A.C. and Martin, B.R. (2007) Genetic approaches identify differential roles for a4β2 nicotinic receptors in acute models of antinociception in mice. J. Pharmacol. Exp. Ther., 321(3): 1161-1169. [Crossref] [PubMed]

19. AlSharari, S.D., Carroll, F.I., McIntosh, J.M. and Damaj, M.I. (2012) The antinociceptive effects of nicotinic partial agonists varenicline and sazetidine-a in murine acute and tonic pain models. J. Pharmacol. Exp. Ther., 342(3): 742-749. [Crossref] [PubMed] [PMC]