Association of single nucleotide polymorphisms in the fatty acid synthase, LOC514211, and fat mass and obesity-associated genes with milk traits in Indonesian-Holstein dairy cattle

Research (Published online: 30-07-2019)

35. Association of single nucleotide polymorphisms in the fatty acid synthase, LOC514211, and fat mass and obesity-associated genes with milk traits in Indonesian-Holstein dairy cattle

Amalia Puji Rahayu, Tety Hartatik, Agung Purnomoadi and Edy Kurnianto

Veterinary World, 12(7): 1160-1166

Amalia Puji Rahayu: Department of Animal Science, Faculty of Animal and Agricultural Sciences, Diponegoro University, Semarang 50275, Indonesia; Agriculture, Fisheries and Food Center of Semarang Regency, Ungaran 50514, Indonesia.

Tety Hartatik: Department of Animal Breeding and Reproduction, Faculty of Animal Sciences, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.

Agung Purnomoadir: Department of Animal Science, Faculty of Animal and Agricultural Sciences, Diponegoro University, Semarang 50275, Indonesia.

Edy Kurnianto: Department of Animal Science, Faculty of Animal and Agricultural Sciences, Diponegoro University, Semarang 50275, Indonesia.

doi: 10.14202/vetworld.2019.1160-1166

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Article history: Received: 13-05-2019, Accepted: 25-06-2019, Published online: 29-07-2019

Corresponding author: Edy Kurnianto

E-mail: kurniantoedy17@gmail.com

Citation: Rahayu AP, Hartatik T, Purnomoadi A, Kurnianto E (2019) Association of single nucleotide polymorphisms in the fatty acid synthase, LOC514211, and fat mass and obesity-associated genes with milk traits in Indonesian-Holstein dairy cattle, Veterinary World, 12(7): 1160-1166.

Abstract

Aim: The study aimed to identify fatty acid synthase (FASN), LOC514211, and fat mass and obesity-associated (FTO) gene polymorphisms and to investigate their associations with milk traits in an Indonesian-Holstein dairy cow population.

Materials and Methods: A total of 100 Indonesian-Holstein cows consisting of 50 heads (0th generation; G0) and 50 heads of their daughters (1st generation; G1) were used. Polymerase chain reaction-restriction fragment length polymorphism was performed to genotype three single nucleotide polymorphisms: rs41919985 in the FASN gene, rs42688595 in the LOC514211 gene, and g.1371T>A in the FTO gene.

Results: FASN rs41919985 was associated with milk protein percentage (p<0.05), FTO g.1371T>A was associated with milk fat percentage (p<0.05), and LOC514211 rs42688595 was not associated with any trait (p>0.05). Heterozygote variants showed a higher protein percentage for FASN and the highest fat percentage for FTO. These associations were consistent in the G0 and G1 populations.

Conclusion: Our results indicate that the milk protein and fat percentages can be improved by increasing the frequency of the AG genotype of FASN and the AT genotype of FTO, respectively.

Keywords: fat mass and obesity-associated, fatty acid synthase, Indonesian-Holstein cattle, LOC514211, milk traits.