doi: 10.14202/vetworld.2019.860-867
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Article history: Received: 16-02-2019, Accepted: 13-05-2019, Published online: 21-06-2019
Corresponding author: Widya Paramita Lokapirnasari
E-mail: widyaparamitalokapirnasari@gmail.com
Citation: Lokapirnasari WP, Pribadi TB, Al Arif A, Soeharsono S, Hidanah S, Harijani N, Najwan R, Huda K, Wardhani HCP, Rahman NFN, Yulianto AB (2019) Potency of probiotics Bifidobacterium spp. and Lactobacillus casei to improve growth performance and business analysis in organic laying hens, Veterinary World, 12(6): 860-867.Aim: This study aimed to determine the use of probiotics Bifidobacterium spp. and Lactobacillus casei as alternative antibiotic growth promoters (AGPs) to improve growth performance and business analysis.
Materials and Methods: This study used a completely randomized factorial design. The first factor was the time of administration (1, 2, 3, and 4 weeks) and the second was the use of probiotics (control without probiotics; 0.1% AGP and 0.5% Bifidobacterium spp. + 0.25% L. casei). One hundred and eighty laying hens (Lohmann strain), of 30 weeks old, were divided into 12 treatment groups, composed of five replicates, each consisting of three laying hens.
Results: The results showed that using 0.5% Bifidobacterium spp. + 0.25% L. casei in weeks 1 and 2 showed the lowest feed intake (FI) (112.11-112.19 g/day), the highest egg weight (60.28 g) in the 1st week, the lowest feed conversion ratio (FCR) (2.21-2.23), and highest feed efficiency (44.75-45.25%) for 3-4 weeks, and the highest hen-day production (86.66-86.90%) for 3-4 weeks and the most profitable business analysis (IDR. 30,353).
Conclusion: Based on the results, it can be concluded that the addition of 0.5% Bifidobacterium spp. + 25% L. casei probiotics can be used as a substitute for AGP; it can reduce the FI and FCR, increasing egg weight, feed efficiency, and hen-day production, as well as illustrating the results of the most profitable business analysis.
Keywords: antibiotic growth promoters, business analysis, growth performance, hen-day production, probiotics.
1. EC Council Regulation. (1999) EC Council Regulation No. 1804/99 of July 1999 Supplementing Regulation (EEC) No. 2092/91 on Organic Production of Agricultural Products. Official Journal L. 222128.
2. Permentan. (2017) Regulation of the Minister of Agriculture Republic Indonesia Nomor14/PERMENTAN/PK.350/5/2017 about Classification of Animal Medicine. Ministry of Agriculture, Jakarta.
3. Kochewad, S.A., Chahande, J.M., Kanduri, A.B., Deshmukh, D.S., Ali, S.A. and Patil, V.M. (2009) Effect of probiotic supplementation on growth parameters of Osmanabadi kids. Vet. World, 2(1): 29.
4. Rowghani, E., Arab, M. and Akbarian, A. (2007) Effects of probiotic and other feed additives on performance and immune response of broiler chicks. Int. Poult. Sci., 6(4): 261-265. [Crossref]
5. Shareef, A.M. and Al-Dabbagh, A.S. (2009) Effect probiotic (Saccharomyces cerevisiae) on performance of broiler chicks. Iraqi J. Vet. Sci., 23(1): 23-29.
6. Getachew, T. (2016) A review on effects of probiotic supplementation in poultry performance and cholesterol levels of egg and meat. J. World Poult. Res., 6(1): 31-36.
7. Lokapirnasari, W.P., Rahmawati, A. and Eliyani, H. (2016) Potential addition of Lactobacillus casei and Lactobacillus rhamnosus lactic acid bacteria to feed consumption and feed conversion of broiler. J. Agro Vet., 5(1): 43-49.
8. Lokapirnasari, W.P., Sahidu, A.M., Soepranianondo, K., Supriyanto, A., Yulianto, A.B. and Al Arif, A. (2018) Potency of lactic acid bacteria isolated from Balinese bovine (Bos sondaicus) intestinal waste from slaughterhouse to improve nutrient content of wheat pollard as animal feedstuff by fermentation process. Vet. World, 11(8): 1127-1134. [Crossref] [PubMed] [PMC]
9. Chichlowski, M., Croom, J., McBride, B.W., Daniel, L., Davis, G. and Koci, M.D. (2007) Direct-fed microbial primalac and salinomycin modulate whole-body and intestinal oxygen consumption and intestinal mucosal cytokine production in the broiler chick. Poult. Sci., 86(6): 1100-1106. [Crossref] [PubMed]
10. Forte, C., Acuti, G., Manuali, E., Proietti, P.C., Pavone, S., Trabalza-Marinucci, M., Moscati, L., Onofri, A., Lorenzetti, C. and Franciosini, M.P. (2016) Effects of two different probiotics on microflora, morphology, and morphometry of gut in organic laying hens. Poult. Sci., 95(11): 2528-2535. [Crossref] [PubMed]
11. Abdelqader, A., Al-Fataftah, A.R. and Das, G. (2013) Effects of dietary Bacillus subtilis and inulin supplementation on performance, eggshell quality, intestinal morphology and microflora composition of laying hens in the late phase of production. Anim. Feed Sci. Technol., 179(1-4): 103-111. [Crossref]
12. Knap, I., Kehlet, A.B., Bennedsen, M., Mathis, G.F., Hofacre, C.L., Lumpkins, B.S., Jensen, M.M., Raun, M. and Lay, A. (2011) Bacillus subtilis (DSM17299) significantly reduces Salmonella in broilers. Poult. Sci., 90(8): 1690-1694. [Crossref] [PubMed]
13. Jeong, J.S. and Kim, I.H. (2014) Effect of Bacillus subtilis C-3102 spores as a probiotic feed supplement on growth performance, noxious gas emission, and intestinal microflora in broilers. Poult. Sci., 93(12): 3097-3103. [Crossref] [PubMed]
14. Li, S.P., Zhao, X.J. and Wang, J.Y. (2009) Synergy of Astragalus polysaccharides and probiotics (Lactobacillus and Bacillus cereus) on immunity and intestinal microbiota in chicks. Poult. Sci., 88(3): 519-525. [Crossref] [PubMed]
15. Watkins, B.A., Miller, B.F. and Neil, D.H. (1982) In vivo inhibitory effects of Lactobacillus acidophilus against pathogenic Escherichia coli in gnotobiotic chicks. Poult. Sci., 61(7): 1298-1308. [Crossref] [PubMed]
16. Pambuka, S.R., Sjofjan, O. and Radiati, L.E. (2014) Effect of liquid probiotics mixed culture supplements through drinking water on laying hens performance and yolk cholesterol. J. Worlds Poult. Res., 4(1): 5-9.
17. Nahashon, S.N., Nakaue, H.S. and Mirosh, L.W. (1996) Performance of single comb white leghorn fed a diet supplemented with a live microbial during the growth and egg laying phases. Anim. Feed Sci. Technol., 57(1-2): 25-38. [Crossref]
18. Yousefi, M. and Karkoodi, K. (2007) Effect of probiotic thepax® and Saccharomyces cerevisiae supplementation on performance and egg quality of laying hens. Int. J. Poult. Sci., 6(1): 52-54. [Crossref]
19. Mahdavi, A.H., Rahman, H.R. and Pourreza, J. (2005) Effect of probiotic supplements on egg quality and laying hen's performance. Int. J. Poult. Sci., 4(7): 488-492. [Crossref]
20. Ramasamy, K., Abdullah, N., Wong, M.C., Karuthan, C. and Ho, Y.W. (2010) Bile salt deconjugation and cholesterol removal from media by Lactobacillus strains used as probiotics in chickens. J. Sci. Food Agric., 90(1): 65-69. [Crossref] [PubMed]
21. Richards, M.P. (2003) Genetic regulation of feed intake and energy balance in poultry. Poult. Sci., 82(6): 907-916. [Crossref] [PubMed]
22. Lindholm-Perry, A.K., Sexten, A.K., Kuehn, L.A., Smith, T.P., King, D.A., Shackelford, S.D., Wheeler, T.L., Ferrell, C.L., Jenkins, T.G., Snelling, W.M. and Freetly, H.C. (2011) Association, effects and validation of polymorphisms within the NCAPG-LCORL locus located on BTA6 with feed intake, gain, meat and carcass traits in beef cattle. BMC Genet., 12(1): 103. [Crossref] [PubMed] [PMC]
23. Wolc, A., Arango, J., Settar, P., Fulton, J.E., O'sullivan, N.P., Preisinger, R., Habier, D., Fernando, R., Garrick, D.J., Hill, W.G. and Dekkers, J.C.M. (2012) Genome-wide association analysis and genetic architecture of egg weight and egg uniformity in layer chickens. Anim. Genet., 43(1): 87-96. [Crossref] [PubMed]
24. Edens, F.W. (2003) An alternative for antibiotic Se in poultry: Probiotics. Rev. Bras. Cienc. Avic., 5(2): 75-97. [Crossref]
25. Daneshyar, M., Kermanshahi, H. and Golian, A. (2009) Changes of biochemical parameters and enzyme activities in broiler chickens with cold-induced ascites. Poult. Sci., 88(1): 106-110. [Crossref] [PubMed]
26. Davis, G.S. and Anderson, K.E. (2002) The effects of feeding the direct-fed microbial, primalac, on growth parameters and egg production in single white leghorn hens. Poult. Sci., 81(6): 755-759. [Crossref] [PubMed]
27. Khan, S.H., Atif, M., Mukhtar, N., Rehman, A. and Fareed, G. (2011) Effects of supplementation of multi-enzyme and multi-species probiotic on production performance, egg quality, cholesterol level and immune system in laying hens. J. Appl. Anim. Res., 39(4): 386-398. [Crossref]
28. Inatomi, T. (2016) Laying performance, immunity and digestive health of layer chickens fed diets containing a combination of three probiotics. Sci. Postprint, 1(2): e00058. [Crossref]
29. Yan, W., Sun, C., Yuan, J. and Yang, N. (2017) Gut metagenomic analysis reveals prominent roles of Lactobacillus and cecal microbiota in chicken feed efficiency. Sci. Rep., 7(3): 45308. [Crossref] [PubMed]
30. Yoruk, M.A., Gul, M., Hayirli, A. and Macit, M. (2004) The effects of supplementation of humate and probiotic on egg production and quality parameters during the late laying period in hens. Poult. Sci., 83(1): 84-88. [Crossref] [PubMed]
31. Chen, Y.C., Nakthong, C. and Chen, T.C. (2005) Improvement of laying hen performance by dietary prebiotic chicory oligofructose and inulin. Int. J. Poult. Sci., 4(2): 103-108. [Crossref]
32. Stanley, D., Geier, M.S., Denman, S.E., Haring, V.R., Crowley, T.M., Hughes, R.J. and Moore, R.J. (2013) Identification of chicken intestinal microbiota correlated with the efficiency of energy extraction from feed. Vet. Microbiol., 164(1-2): 85-92. [Crossref] [PubMed]
33. Turnbaugh, P.J., Backhed, F., Fulton, L. and Gordon, J.I. (2008) Diet-induced obesity is linked to marked but reversible alterations in the mouse distal gut microbiome. Cell Host Microbe, 3(4): 213-223. [Crossref] [PubMed] [PMC]
34. Haddadin, M.S.Y., Abdulrahim, S.M., Hashlamoun, E.A.R. and Robinson, R.K. (1996) The effect of Lactobacillus acidophilus on the production and chemical composition of hen's eggs. Poult. Sci., 75(4): 491-494. [Crossref] [PubMed]
35. Lokapirnasari, W.P., Dewi, A.R., Fathinah, A., Hidanah, S., Harijani, N., Soeharsono , S., Karimah, B. and Andriani, A.D. (2017) Effect of probiotic supplementation on organic feed to alternative antibiotic growth promoter on production performance and economics analysis of quail. Vet. World, 10(12): 1508-1514. [Crossref] [PubMed] [PMC]
36. Panda, A.K., Reddy, M.R., Rao, S.V.R. and Praharaj, N.K. (2003) Production performance, serum/yolk cholesterol and immune competence of white leghorn layers as influenced by dietary supplementation with probiotic. Trop. Anim. Health Prod., 35(1): 85-94. [Crossref]