Aim: The present study was designed to demonstrate the autoligation (AL) of vas deferens and the excision-ligation (EL) technique to generate vasectomized rams to reduce the complications, operative time, and price of the vasectomy techniques.

Materials and Methods: A total of 12 healthy and mature Iraqi Awassi rams were used, which divided into two groups, six rams for each one. The former group was performed the EL technique while the latter group, the AL of vas deferens technique was used.

Results: The results of the present study found that both techniques were same with the reproductive efficient examinations that mean the two techniques had same ability to close the male genital passage for teaser rams preparation. However, the methods were different with the histopathological changes, operation time, prices, and complications, which were minor in the AL of vas deferens compared with the EL technique.

Conclusion: The AL technique of vas deferens to prepare teaser animal is recommended over the EL technique due to different aspects such as cost, fewer complications, and active teaser for a long period are the main aspects of AL technique.

Keywords: ram infertility, testes, vas occlusion, vasectomy techniques.

1. Soren, S., Singh, S.V. and Singh, P. (2016) Influence of season on seminal antioxidant enzymes in Karan Fries bulls under tropical climatic conditions. Turk. J. Vet. Anim. Sci., 40(6): 797-802. [Crossref]

2. Dos Santos-Neto, P., Garcia-Pintos, C., Pinczak, A. and Menchaca, A. (2015) Fertility obtained with different progestogen intravaginal devices using short-term protocol for fixed-time artificial insemination (FTAI) in sheep. Livest. Sci., 182: 125-128. [Crossref]

3. Fornazari, R.R., Mateus, O., Correia, T.M., Quintas, H., Mauricio, R., Conradi, A. and Valentim, R. (2018) Estrus synchronization and artificial insemination with fresh and chilled semen in Assaf ewes. Agric. Sci., 9(1): 8-22. [Crossref]

4. Galarza, D., de Guevara, M.L., Beltran-Brena, P., Sanchez-Calabuig, M., Lopez-Sebastian, A., Santiago-Moreno, J. and Rizos, D. (2019) 137 assessment of fertilizing ability of merino ram semen cold stored up to 48 h by heterologous IVF of bovine oocytes. Reprod. Fertil. Dev., 31(1): 194. [Crossref]

5. Ascari, I.J., Alves, N.G., Alves, A.C., Garcia, I.F.F. and Junqueira, F.B. (2016) Resumption of cyclic ovarian activity in postpartum ewes: A review. Semin. Cienc. Agrar., 37(2): 1101-1116. [Crossref]

6. Gursu, G. and Aygun, T. (2014) Serum calcium, potassium, phosphorus and cobalt levels of awassi ewes maintained at village conditions during lactation period. APCBEE Procedia, 8: 6-10. [Crossref]

7. Beynon, S.E., Slavov, G.T., Farre, M., Sunduimijid, B., Waddams, K., Davies, B. and Newbold, C.J. (2015) Population structure and history of the Welsh sheep breeds determined by whole-genome genotyping. BMC Genet., 16(1): 65. [Crossref] [PubMed] [PMC]

8. Galal, S., Gursoy, O. and Shaat, I. (2008) Awassi sheep as a genetic resource and efforts for their genetic improvement-a review. Small Rumin. Res., 79(2-3): 99-108. [Crossref]

9. Talafha, A.Q. and Ababneh, M.M. (2011) Awassi sheep reproduction and milk production. Trop. Anim. Health Prod., 43(7): 1319-1326. [Crossref] [PubMed]

10. Sokal, D.C. and Labrecque, M. (2009) Effectiveness of vasectomy techniques. Urol. Clin. North Am., 36(3): 317-329. [Crossref] [PubMed]

11. Julanova, N., Tagayev, O., Julanov, M., Alimbekova, M., Koibagarov, K., Ussenbekov, Y. and Mautenbaev, A. (2016) The method of preparation and use of vasectomized stallions to regulate the sexual function in mares during hippodrome testing. Acta Vet. Brno, 85(1): 63-69. [Crossref]

12. Godke, R.A., Lambeth, V.A., Kreider, J.L. and Root, R.G. (1979) A simplified technique of vasectomy for heat-check boars. Vet. Med. Small Anim. Clin., 74(7): 1027-1029.

13. Shih, G., Turok, D.K. and Parker, W.J. (2011) Vasectomy: The other (better) form of sterilization. Contraception, 83(4): 310-315. [Crossref] [PubMed]

14. Lofstedt, R.M. (1982) Vasectomy in ruminants: A cranial mid-scrotal approach. J. Am. Vet. Med. Assoc., 181(4): 373-375.

15. Barone, M.A., Irsula, B., Chen-Mok, M., Sokal, D.C., Coutinho, E.M. and Labrecque, M. (2004) Effectiveness of vasectomy using cautery. BMC Urol. J., 4(10): 12-15. [Crossref] [PubMed] [PMC]

16. Sokal, D., Irsula, B., Chen-Mok, M., Labrecque, M. and Barone, M.A. (2004) A comparison of vas occlusion techniques: Cautery more effective than ligation and excision with fascial interposition. BMC Urol. J., 4(12): 1-8. [Crossref] [PubMed] [PMC]

17. Cai, Z.S., Ping, Z.S. and Chuan, Y.R. (1992) Intravasal injection of formed in place silicone rubber as a method of vas occlusion. Int. J. Androl., 15(6): 460-464. [Crossref]

18. Cook, L.A., Pun, A., Gallo, M.F., Lopez, L.M. and Van Vliet, H.A.A.M. (2007) Scalpel versus no-scalpel incision for vasectomy. Cochrane Database Syst. Rev., 18(2): CD004112. [Crossref] [PubMed]

19. Kong, L.S., Huang, A.P., Deng, X.Z. and Yang, Z.W. (2004) Quantitative (stereological) study of the effects of vasectomy on spermatogenesis in rabbits. J. Anat., 205(2): 147-156. [Crossref] [PubMed] [PMC]

20. Ibrahiem, H.K. (2014) Evaluation study for auto-ligation of vas-deferens method to teaser bucks preparation. Int. J. Adv. Biol. Res., 4(2): 206-210.

21. Baiee, F.H., Al-Fatlawy, M.A.T., Jeber, Z.K.M. and Al-Azaam, A.H. (2012) Twins Production in Awassi Ewes Out of Breeding Season. The 2nd International Conference of Faculty of Veterinary Medicine, University of Kufa, Iraq. p34-42.

22. Karaca, S., Erdogan, S. and Yilmaz, A. (2015) The relationship between sexual behaviors and serum testosterone concentrations in Norduz rams. J. Anim. Prod., 56(2): 8-13.

23. Baiee, F., Haron, W., Yusoff, R., Omar, A., Yimer, N., Hammadi, S., Ahmedeltayeb, A. and Kaka, A. (2018) Modification of electro-ejaculation technique to minimize discomfort during semen collection in bulls. Pak. J. Zool., 50(1): 83-89. [Crossref]

24. Santos, S.I., Sanchez-Davila, F., Vazquez-Armijo, J.F., Ledezma-Torres, R.A., del Bosque-Gonzalez, A.S., Palomera, C.L. and Bernal-Barragan, H. (2015) Changes in sexual behavior and semen quality associated with age and type of enclosure of saint croix rams in different seasons of the year. Ital. J. Anim. Sci., 14(4): 3890. [Crossref]

25. Rekha, A., Zohara, B.F., Bari, F. and Alam, M.G.S. (2016) Comparison of commercial triladyl extender with a tris-fructose-egg-yolk extender on the quality of frozen semen and pregnancy rate after transcervical AI in Bangladeshi indigenous sheep (Ovis aries). Small Rumin. Res., 134: 39-43. [Crossref]

26. Janett, F., Hussy, D., Lischer, C., Hassig, M. and Thun, R. (2001) Semen characteristics after vasectomy in the ram. Theriogenology, 56(3): 485-491. [Crossref]

27. Batista, M., Calero, P., Rodriguez, F., Gonzalez, F., Cabrera, F. and Gracia, A. (2002) Structural changes in the testes and epididymides of bucks 16 weeks after bilateral vasectomy. Vet. Rec., 151(24): 740.

28. Seppan, P. and Krishnaswamy, K. (2014) Long-term study of vasectomy in Macaca radiata-histological and ultrasonographic analysis of testis and duct system long-term study of vasectomy in Macaca radiata-histological and ultrasonographic analysis of testis and duct system. Syst. Biol. Reprod. Med., 60(3): 151-160. [Crossref] [PubMed]

29. Lee, J.Y., Lee, T.Y., Park, H.Y., Choi, H.Y., Yoo, T.K. and Moon, H.S. (2011) Efficacy of epididymectomy in treatment of chronic epididymal pain: A comparison of patients with and without a history of vasectomy. Urology, 77(1): 177-182. [Crossref] [PubMed]

30. Al-Maghrebi, M., Kehinde, E.O. and Anim, J.T. (2011) Survivin down regulation is associated with vasectomy-induced spermatogenic damage and apoptosis. Med. Princ. Pract., 20(5): 449-454. [Crossref] [PubMed]

31. Byrne, P.A.C., Evans, W.D. and Rajan, K.T. (1997) Does vasectomy predispose to osteoporosis? Br. J. Urol., 79(4): 599-601. [Crossref]

32. Ren, L., Weng, Q., Kishimoto, M., Watanabe, G. and Taya, K. (2011) Effect of short period vasectomy on FSH, LH, inhibin and testosterone secretions, and sperm motility in adult male rats. Exp. Anim. J., 60(1): 47-56. [Crossref] [PubMed]

33. Flickinger, C.J., Howards, S.S. and Herr, J.C. (1995) Effects of vasectomy on the epididymis. Microsc. Res. Tech. J., 30(1): 82-100. [Crossref] [PubMed]

34. Seamans, Y. and Harner-Jay, C.M. (2007) Modelling cost-effectiveness of different vasectomy methods in India, Kenya, and Mexico. Cost Eff. Resour. Alloc., 5(1): 1-8. [Crossref] [PubMed] [PMC]

35. Diegelmann, R.F. and Evans, M.C. (2004) Wound healing: An overview of acute, fibrotic and delayed healing. Front. Biosci., 9(1): 283-289. [Crossref]