Aim: The present cross-sectional study aimed at assessing the seroprevalence of Neospora caninum infection both at herd and within herd and at determining risk factors that are associated with its seropositivity.

Materials and Methods: A total of 90 cows distributed over seven herds located in two North-Eastern Algerian provinces were blood sampled in order to be tested for the presence of antibodies against N. caninum using a commercially available enzyme-linked immunosorbent assay kit.

Results: The individual seroprevalence of N. caninum was found to be 12.22%, and six of the seven herds tested had at least one seropositive cow. The logistic regression model revealed that abortion (odds ratio [OR]=29.15) and parity (OR=7.38) were positively associated with the seropositivity of animals on an individual basis.

Conclusion: The study confirms the existence of N. caninum infection in cattle in North-Eastern Algeria. However, a widespread infection rate of 85.71% and its significant statistical association with previous abortion (OR=29.15) need further investigations.

Keywords: cows, enzyme-linked immunosorbent assay, Neospora caninum, North-Eastern Algeria, reproduction.

1. Jin, X., Li, G., Zhang, X., Gong, P., Yu, Y. and Li, J. (2017) Activation of a Neospora caninum EGFR-like kinase facilitates intracellular parasite proliferation. Front. Microbiol., 8: 1980. [Crossref] [PubMed] [PMC]

2. Dubey, J.P., Schares, G. and Ortega-Mora, L.M. (2007) Epidemiology and control of neosporosis and Neospora caninum. Clin. Microbiol. Rev., 20(2): 323-367. [Crossref] [PubMed] [PMC]

3. Bjerkas, I., Mohn, S.F. and Presthus, J. (1984) Unidentified cyst-forming sporozoan causing encephalomyelitis and myositis in dogs. Z. Parasitenkd., 70(2): 271-274. [Crossref]

4. Dubey, J.P., Carpenter, J.L., Speer, C.A., Topper, M.J. and Uggla, A.N.D. (1988) Newly recognized fatal protozoan disease of dogs. J. Am. Vet. Med. Assoc., 192(9): 1269-1285.

5. Almeria, S. and Lopez-Gatius, F. (2013) Bovine neosporosis: Clinical and practical aspects. Res. Vet. Sci., 95(2): 303-309. [Crossref] [PubMed]

6. Donahoe, S.L., Lindsay, S.A., Krockenberger, M., Phalen, D. and Slapeta, J. (2015) A review of neosporosis and pathologic findings of Neospora caninum infection in wildlife. Int. J. Parasitol Parasites Wildl., 4(2): 216-238. [Crossref] [PubMed] [PMC]

7. Pooley, F., Remnant, J. and Wapenaar, W. (2014) Neospora in cattle and dogs: An update. Livestock, 19(3): 153-157. [Crossref]

8. Reichel, M.P., McAllister, M.M., Pomroy, W.E., Campero, C., Ortega-Mora, L.M. and Ellis, J.T. (2014) Control options for Neospora caninum is there anything new or are we going backward? Parasitology, 141(11): 1455-1470. [Crossref] [PubMed]

9. McAllister, M.M. (2016) Diagnosis and control of bovine neosporosis. Vet. Clin. North Am. Food Anim. Pract., 32(2): 443-463. [Crossref] [PubMed]

10. McAllister, M.M., Dubey, J.P., Lindsay, D.S., Jolley, W.R., Wills, R.A. and McGuire, A.M. (1998) Rapid communication: Dogs are definitive hosts of Neospora caninum. Int. J. Parasitol., 128(9): 1473-1479. [Crossref]

11. Gondim, L.F., McAllister, M.M., Pitt, W.C. and Zemlicka, D.E. (2004) Coyotes (Canis latrans) are definitive hosts of Neospora caninum. Int. J. Parasitol., 34(2): 159-161. [Crossref] [PubMed]

12. King, J.S., Slapeta, J., Jenkins, D.J., Al-Qassab, S.E., Ellis, J.T. and Windsor, P.A. (2010) Australian dingoes are definitive hosts of Neospora caninum. Int. J. Parasitol., 40(8): 945-950. [Crossref] [PubMed]

13. Moore, D.P. and Venturini, M.C. (2018) Neospora. In: Parasitic Protozoa of Farm Animals and Pets. Springer, Switzerland. p125-148. [Crossref]

14. Campero, L.M., Minke, L., More, G., Rambeaud, M., Bacigalupe, D., Moore, D.P., Hecher, Y., Campero, C.M., Schares, G. and Venturini, M.C. (2015) Evaluation and comparison of serological methods for the detection of bovine neosporosis in Argentina. Rev. Argent. Microbiol., 47(4): 295-301. [Crossref] [PubMed]

15. Guido, S., Katzer, F., Nanjiani, I., Milne, E. and Innes, E.A. (2016) Serology-based diagnostics for the control of bovine neosporosis. Trends Parasitol., 32(2): 131-143. [Crossref] [PubMed]

16. Ghalmi, F., China, B., Kaidi, R., Daube, G. and Losson, B. (2008) Detection of Neospora caninum in dog organs using real-time PCR systems. Vet. Parasitol., 155(1-2): 161-167. [Crossref] [PubMed]

17. Dechicha, A., Gharbi, S., Kebbal, S., Chatagnon, G., Tainturier, D., Ouzrout, R. and Guetarni, D. (2010) Serological survey of etiological agents associated with abortion in two Algerian dairy cattle breeding farms. J. Vet. Anim. Health, 2(1): 1-5.

18. Ghalmi, F., China, B., Ghalmi, A., Hammitouche, D. and Losson, B. (2012) Study of the risk factors associated with Neospora caninum seroprevalence in Algerian cattle populations. Res. Vet. Sci., 93(2): 655-661. [Crossref] [PubMed]

19. Achour, K., Ben-Mahdi, M.H., Akkou, M. and Teniou, R. (2012) Seroprevalence de Neospora caninum dans les elevages bovins laitiers de la region centre nord de l'Algerie. Rev. Sci. Tech. Int. Epiz., 31(3): 953-958.

20. Bouaziz, O., Dib, A.L., Aimeur, R., Lakhdara, N., Bouaziz, A. and Brerhi, E. (2013) Seroprevalence of Neospora caninum in dairy cattle in Eastern Algeria. Ann. Biol. Res., 4(11): 76-77.

21. Thrusfield, M. (2008) Veterinary Epidemiology. 3rd ed. United Kindom: Blackwell Publishing Company. p266-284, 610.

22. Fort, M., Edelsten, M., Maley, S. and Innes, E. (2015) Seroepidemiological study of Neospora caninum in beef and dairy cattle in La Pampa, Argentina. Acta Parasitol.,60(2): 275-282. [Crossref] [PubMed]

23. Chatziprodromidou, I.P. and Apostolou, T. (2018) Diagnostic accuracy of enzyme-linked immunosorbent assay (ELISA) and immunoblot (IB) for the detection of antibodies against Neospora caninum in milk from dairy cows. Epidemiol. Infect., 146(5): 577-583. [Crossref] [PubMed]

24. Klauck, V., Machado, G., Pazinato, R., Radavelli, W.M., Santos, D.S., Berwaguer, J. C., Braunig, P., Vogel, F.F. and Da Silva, A.S. (2016) Relation between Neospora caninum and abortion in dairy cows: Risk factors and pathogenesis of disease. Microb. Pathol., 92: 46-49. [Crossref] [PubMed]

25. Llano, H.A.B., Guimaraes, M.S., Soares, R.M., Polo, G. and da Silva, A.C. (2018) Seroprevalence and risk factors for Neospora caninum infection in cattle from the Eastern Antioquia, Colombia. Vet. Anim. Sci., 6: 69-74. [Crossref]

26. Martinez, B.A.F., Leotti, V.B., Borba, M.R., Silva, G.D.S. and Corbellini, L.G. (2017) Can hierarchical modeling improve our understanding of bovine abortion due to Neospora caninum infection? Vet. Parasitol., 237: 77-82. [Crossref] [PubMed]

27. Schetters, T., Heuer, C., Nicholson, C., Russel, D. and Weston, J. (2004) Field study in dairy cattle from New Zealand. Intervet symposium: Bovine neosporosis. Vet Parasitol., 125(1-2): 137-146. [Crossref] [PubMed]

28. Jenkins, M., Baszler, T., Bjorkman, C., Schares, G. and Williams, D. (2002) Diagnosis and seroepidemiology of Neospora caninum-associated bovine abortion. Int. J. Parasitol., 32(5): 631-636. [Crossref]

29. Dubey, J.P., Hemphill, A., Calero-Bernal, R. and Schares, G. (2017) Neosporosis in Animals. CRC Press, Taylor & Francis Group. London. p203-225. [Crossref]

30. Moore, D.P., Perez, A., Agliano, S., Brace, M., Canton, G., Cano, D., Leunda, M.R., Odeon, A.C., Odriozola, E. and Campero, C.M. (2009) Risk factors associated with Neospora caninum infections in cattle in Argentina. Vet. Parasitol., 161(1-2): 122-125. [Crossref] [PubMed]

31. Georgieva, D.A., Prelezov, P.N. and Koinarski, V.T.S. (2006) Neospora caninum and neosporosis in animals: A review. Bulg. J. Vet. Med., 9(1): 1-26.

32. Moore, D.P., Konrad, J.L., San Martino, S., Reichel, M.P., Cano, D.B., Mendez, S., Spath, E.J.L., Odeon, A.C., Crudeli, G. and Campero, C.M. (2014) Neospora caninum serostatus is affected by age and species variables in cohabiting water buffaloes and beef cattle. Vet. Parasitol., 203(3-4): 259-263. [Crossref] [PubMed]