Background and Aim: Horses have a strategic and vital role to play in the lives of the people of Sumba Island, East Nusa Tenggara Province. They act as social animals that are involved in death ceremonies, horse races, and during pasola, thereby supporting tourism, and are given away as dowry in wedding ceremonies. This study aimed to investigate the prevalence of trypanosomiasis among horses in four districts of Sumba Island by examining clinical symptoms and detecting parasites, antibodies, and other factors that are related to Trypanosoma evansi infection in horses.

Materials and Methods: We studied a total of 211 horses that belonged to 88 clinical hobby breeders. Giemsa-colored smears and serum were examined in order to detect antibodies using card-agglutination tests (CATT). The study was conducted during the rainy season that lasted from January to March 2017. Potential risk factors such as the species, sex, origin of the livestock, how the livestock were maintained, and the farmers' knowledge concerning trypanosomiasis were recorded using questionnaires. Data were collected annually for three years from 2010-2012 and repeatedly analyzed by a Chi-square test.

Results: Clinical signs of trypanosomiasis were found in 34 horses; blood smears were examined using Giemsa staining and negative preparations were obtained at a frequency of 0.0% (0/211). The CATT results generally showed that 13.3% (28/211) of the samples were seropositive for antibodies to T. evansi; the highest percentage, 16.67% (8/48), of seropositivity was found in the West Sumba District, and the lowest, 12.0% (5/50), was found in Southwest Sumba. The incidence of trypanosomiasis was higher (75% [21/28]) among female hip horses; horses with 1-5 years of experience were more susceptible to a T. evansi infection (46.4% [13/28]). In general, farmers on Sumba Island knew of trypanosomiasis (89.8% [79/88]), and 69.3% (61/88) of the farmers reported that their livestock was sick. This study was the first serological study conducted on trypanosomiasis in horses of Sumba Island after the surra outbreak in 2010-2012. There were 3% of farmers who were willing to provide the government with information on implementing a prevention program and controlling the spread of surra on the island.

Conclusion: The diagnoses of surra disease were made based on clinical symptoms and parasitological examinations. CATTs could be used to diagnose T. evansi infection in horses.

Keywords: CATT, horses, Sumba Island, Trypanosoma evansi.

1. Dinas Peternakan Kabupaten Sumba Timur. (2015) Laporan Kegiatan Peternakan Tahun 2015. Sumba Timur. Nusa Tenggara Timur.

2. Ng, B.K.Y. and Vancei, B.O.W. (1978) Outbreak of surra in horses and the pathogenesis of anaemia. Kajian Vet., 10(2): 88-98.

3. Desquesnes, M., Holzmuller, P., Lai, D., Dargantes, A., Lun, Z. and Jittaplapong, S. (2013) Trypanosoma evansi and surra: a perspective on origin, history, distribution, taxonomy, morphology, host and pathogenic effect. Biomed. Res. Int., (3): 22. [Crossref] [PubMed] [PMC]

4. Gutierrez, C., Corbera, J.A., Juste, M.C., Doreste, F. and Morales, I. (2006) Clinical, hematological, and biochemical findings in an outbreak of abortion and neonatal mortality associated with Trypanosoma evansi infection in dromedary camels. Ann. N. Y. Acad. Sci., 1081(3): 325-327. [Crossref] [PubMed]

5. Mahmoud, M.E., Abdelfattah, S., Mohamed, M.M. and Eman, K.E.G. (2016) Molecular confirmation of Trypanosoma evansi and Babesia bigemina in cattle from lower Egypt. Pak. Vet. J., 36(4): 409-414.

6. Verloo, D., Magnus, E. and Buscher, P. (2001) General expression of ro tat 1.2 variable antigen type in Trypanosoma evansi isolate from different origin. Vet. Parasitol., 97(3): 183-189. [Crossref]

7. Claes, F., Verloo, D., Maina, M., Urakawa, T., Majiwa, P.A. and Buscher, P. (2005) Recombinant RoTat 1.2 variable surface glycoprotein for diagnosis of Trypanosoma evansi in dromedary camels. Int. J. Parasitol., 35(4): 455-460. [Crossref] [PubMed]

8. Salim, B., Bakheit, M.A. and Sugimoto, C. (2014) Molecular detection of equine trypanosomes in the Sudan. Vet. Parasitol., 200(3-4): 246-250. [Crossref] [PubMed]

9. Elamin, E.A., el Bashir, M.O. and Saeed, E.M. (1998) Prevalence and infection pattern of Trypanosoma evansi in camels in mid-eastern Sudan. Trop. Anim. Health Prod., 30(2): 107-114. [Crossref]

10. Tehseen, S., Jahan, N., Qamar, M.F., Desquesnes, M. and Shahzad, M.I. (2015) Parasitological, serological and molecular survey of Trypanosoma evansi infection in dromedary camels from Cholistan desert, Pakistan. Parasit. Vectors, 8(1): 415. [Crossref] [PubMed] [PMC]

11. Ismael, A., Swelum, A.A., Khalaf, A.F. and Abouheif, M.A. (2014) Clinical, haematological and biochemical alterations associated with an outbreak of theileriosis in dromedaries (Camelus dromedarius) in Saudi Arabia. Pak. Vet. J., 34(2): 209-213.

12. Luckins, A.G. (1988) Trypanosoma evansi in Asia. Parasitol. Today, 4(5): 137-142. [Crossref]

13. Mastra, I.K. (2011) Seroprevalensi trypanosomiasis di pulau sumba, provinsi nusa tenggara barat. Bull. Vet., 23(79): 131-138.

14. Sumbria, D., Singla, L.D., Sharma, A., Moudgil, A.D. and Bal, M.S. (2014) Equine trypanosomiasis in central and western Punjab: prevalence, hemato-biochemical response and associated risk factors. Acta Trop., 138(6): 44-50. [Crossref] [PubMed]

15. Ahmed, A.R. (2008) Epidemiological studies (parasitological, serological and molecular techniques) of Trypanosoma evansi infection in camels (Camelus dromedarius) in Egypt. Vet. World, 1(11): 325-328.

16. Eyob, E. and Matios, L. (2013) Review on camel trypanosomiasis (Surra) due to Trypanosoma evansi: epidemiology and host response. J. Vet. Med. Anim. Health, 5(12): 334-343.

17. Tehseen, S., Jahan, N., Qamar, M.F., Shahzad, M.I. and Desquesnes, M. (2016) Field investigation of Trypanosoma evansi and comparative analysis of diagnostic tests in horses from Bahawalpur, Pakistan. Turk. J. Vet. Anim. Sci., 40(2): 141-146.

18. Bili, F.A.L. (2014) Seroprevalensi Trypanosomiasis (Surra) di Wilayah Nusa Tenggara Timur. Prosiding, Vol. 1 No.5. Penyidikan Penyakit Hewan. Rapat Teknis dan Pertemuan Ilmiah (RATEKPIL) Surveilans Kesehatan Hewan. Dirkeswan-Ditjen Peternakan dan Kesehatan Hewan, Kementerian Pertanian.

19. Bhutto, B., Gadahi, J., Shah, G., Dewani, P. and Arijo, A. (2010) Field investigation on the prevalence of trypanosomiasis in camels in relation to sex, age, breed and herd size. Pak. Vet. J., 30(3): 175-177.

20. Anonymous (2014) Pedoman Pengendalian dan Pemberantasan Penyakit Trypanosomiasis (Surra). Direktorat Kesehatan Hewan, Direktorat Jenderal Peternakan dan Kesehatan Hewan Kementerian Pertanian.