In vitro assessment of time-dependent changes in red cell cytoplasmic antioxidants of donkey blood preserved in citrate phosphate dextrose adenine 1 anticoagulant

Research (Published online: 19-04-2020)

16. In vitro assessment of time-dependent changes in red cell cytoplasmic antioxidants of donkey blood preserved in citrate phosphate dextrose adenine 1 anticoagulant

Okereke Henry Nnamdi, Udegbunam Rita Ijeoma, Nwobi Lotanna Gilbert, Ezeobialu Henry Toochukwu and Udegbunam Sunday Ositadinma

Veterinary World, 13(4): 726-730

Okereke Henry Nnamdi: Department of Veterinary Surgery, Faculty of Veterinary Medicine, University of Nigeria, Enugu State, Nigeria.

Udegbunam Rita Ijeoma: Department of Veterinary Surgery, Faculty of Veterinary Medicine, University of Nigeria, Enugu State, Nigeria.

Nwobi Lotanna Gilbert: Department of Veterinary Physiology and Pharmacology, Faculty of Veterinary Medicine, University of Nigeria, Enugu State, Nigeria.

Ezeobialu Henry Toochukwu: Department of Veterinary Surgery, Faculty of Veterinary Medicine, University of Nigeria, Enugu State, Nigeria.

Udegbunam Sunday Ositadinma: Department of Veterinary Surgery, Faculty of Veterinary Medicine, University of Nigeria, Enugu State, Nigeria; Veterinary Teaching Hospital, Faculty of Veterinary Medicine, University of Nigeria, Enugu State, Nigeria.

doi: www.doi.org/10.14202/vetworld.2020.726-730

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Article history: Received: 20-12-2019, Accepted: 09-03-2020, Published online: 19-04-2020

Corresponding author: Okereke Henry Nnamdi

E-mail: nnamdi.okereke@unn.edu.ng

Citation: Nnamdi OH, Ijeoma UR, Gilbert NL, Toochukwu EH, Ositadinma US (2020) In vitro assessment of time-dependent changes in red cell cytoplasmic antioxidants of donkey blood preserved in citrate phosphate dextrose adenine 1 anticoagulant, Veterinary World, 13(4): 726-730.

Abstract

Background and Aim: Stored blood is continuously exposed to oxidative stress, which affects its antioxidant protective system. Erythrocytes are naturally armed with antioxidant protective capacity. Blood antioxidant system functions to protect the blood cells against oxidative damage by free radicals. However, during storage, blood is continuously exposed to oxidative stress, which affects its antioxidant system. The aim of this work was to investigate alteration in malondialdehyde (MDA) levels, reduced glutathione (glutathione reductase [GSH-Rd]), catalase (CAT), and superoxide dismutase (SOD) activities in stored donkey blood.

Materials and Methods: Blood (250 ml) was drawn from four clinically healthy donkeys into citrate phosphate dextrose adenine 1 blood bags and preserved at 4°C. MDA, GSH-Rd, CAT, and SOD activities were assayed by colorimetric methods, over a period of 42 days.

Results: The result showed that SOD enzyme activities significantly (p<0.05) increased by day 7 post-storage (PS) while MDA levels significantly (p<0.05) increased by day 21 PS. However, activities of GSH-Rd and CAT enzymes decreased (p<0.05) by day 21 PS. Pearson's product-moment correlation showed a negative correlation between the levels of MDA and enzymatic antioxidant markers (CAT and GSH-Rd).

Conclusion: The findings revealed that GSH-Rd and CAT are the primary antioxidant defense markers in donkey red blood cells. The observed alterations in these principal antioxidants suggest a 14 days optimum keeping time of donkey blood for blood banking purposes.

Keywords: blood, citrate phosphate dextrose adenine 1, donkey, malondialdehyde, superoxide dismutase.