Open Access
Research (Published online: 23-04-2020)
22. Molecular identification of Salmonella Typhimurium from village chickens based on invA and spvC genes
Mwanaisha Mkangara, Ernest R. Mbega and Musa Chacha
Veterinary World, 13(4): 764-767

Mwanaisha Mkangara: Department of Sustainable Agriculture and Biodiversity and Ecosystems Management, Nelson Mandela African Institution of Science and Technology, Arusha, Tanzania; Department of Science and Laboratory Technology, Dar es Salaam Institute of Technology, Dar es Salaam, Tanzania.
Ernest R. Mbega: Department of Sustainable Agriculture and Biodiversity and Ecosystems Management, Nelson Mandela African Institution of Science and Technology, Arusha, Tanzania.
Musa Chacha: Department of Sustainable Agriculture and Biodiversity and Ecosystems Management, Nelson Mandela African Institution of Science and Technology, Arusha, Tanzania.

doi: www.doi.org/10.14202/vetworld.2020.764-767

Share this article on [Facebook] [LinkedIn]

Article history: Received: 17-09-2019, Accepted: 11-03-2020, Published online: 23-04-2020

Corresponding author: Mwanaisha Mkangara

E-mail: mkangaram@yahoo.com

Citation: Mkangara M, Mbega ER, Chacha M (2020) Molecular identification of Salmonella Typhimurium from village chickens based on invA and spvC genes, Veterinary World, 13(4): 764-767.
Abstract

Aim: This study aimed to identify Salmonella enterica serovars by polymerase chain reaction (PCR) based on virulence genes invasion A (inv A) and Salmonella plasmid virulence C (spvC).

Materials and Methods: DNA extraction of eight bacteria isolates was done using the PowerSoil® DNA Isolation Kit. The amplification of invA and spvC genes was done using conventional PCR. The positive PCR products were purified using the GeneJET Purification Kit and then sequenced using ABI 3730 XL automated genetic analyzer. The sequences obtained were compared for similarities with other Salmonella serovars deposited on the NCBI GenBank using BLASTN.

Results: Four out of eight samples were amplified by primers FS139/RS141 that target invA gene with products of about 284 bp, and three out of four of the same invA positive samples were also amplified by primers FSPV-1/RSPV-2 targeting spvC with a product of about 571 bp. One sample was not amplified by primers FSPV-1/RSPV-2 as it lacked virulence plasmid. Analysis of sequences indicated 100% homology with closely related serovars of S. enterica subspecies enterica serovar Typhimurium.

Conclusion: Salmonella Typhimurium that contained invA and spvC genes are pathogenic and virulent strains.

Keywords: invasive gene A, polymerase chain reaction, Salmonella plasmid virulence gene, Salmonella Typhimurium, sequencing.