Open Access
Research (Published online: 07-02-2020)
2. Blastocystis spp. subtype 10 infected beef cattle in Kamal and Socah, Bangkalan, Madura, Indonesia
Lucia Tri Suwanti, Yuli Susana, Poedji Hastutiek, Endang Suprihati and Nunuk Dyah Retno Lastuti
Veterinary World, 13(2): 231-237

Lucia Tri Suwanti: Department of Veterinary Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Mulyorejo, Kampus C Unair, Surabaya, 60115, Indonesia; Toxoplasmosis Study Group, Institute of Tropical Diseases, Universitas Airlangga, Jl. Mulyorejo, Campus C Unair, Surabaya 60115, Indonesia.
Yuli Susana: Magister Student, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Mulyorejo, Campus C Unair, Surabaya 60115, Indonesia.
Poedji Hastutiek: Department of Veterinary Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Mulyorejo, Kampus C Unair, Surabaya, 60115, Indonesia.
Endang Suprihati: Department of Veterinary Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Mulyorejo, Kampus C Unair, Surabaya, 60115, Indonesia.
Nunuk Dyah Retno Lastuti: Department of Veterinary Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Mulyorejo, Kampus C Unair, Surabaya, 60115, Indonesia.

doi: www.doi.org/10.14202/vetworld.2020.231-237

Share this article on [Facebook] [LinkedIn]

Article history: Received: 08-07-2019, Accepted: 26-12-2019, Published online: 07-02-2020

Corresponding author: Lucia Tri Suwanti

E-mail: tswant@gmail.com

Citation: Suwanti LT, Susana Y, Hastutiek P, Suprihati E, Lastuti NDR (2020) Blastocystis spp. subtype 10 infected beef cattle in Kamal and Socah, Bangkalan, Madura, Indonesia, Veterinary World, 13(2): 231-237.
Abstract

Background and Aim: Blastocystis spp. is a gastrointestinal parasite that can infect both humans and animals and has the potential to become a zoonotic parasite. This study analyzed a subtype (ST) of Blastocystis spp. that had infected beef cattle in Kamal and Socah, Bangkalan, Madura, Indonesia.

Materials and Methods: Fresh stool samples were collected from 108 beef cattle at Kamal and Socah, Bangkalan, Madura, Indonesia. Blastocystis spp. were detected both morphologically and genetically based on the 18S rRNA gene. The morphology of Blastocystis spp. from the stool samples and cultured samples were observed under a light microscope. Blastocystis spp. from 20 positive cultures were amplified through polymerase chain reaction, and the resultant sequences were identified by ST.

Results: One hundred and eight (100%) fecal samples from the fresh or cultured stools were positive morphologically for Blastocystis spp. Molecularly, all 20 of the samples selected for DNA analysis were found to be Blastocystis spp. ST 10.

Conclusion: Based on morphological and molecular detection, the prevalence of Blastocystis spp. infection in beef cattle within Kamal and Socah, Bangkalan, Madura, Indonesia, was high. About 100% were non-zoonotic parasites. This was the first report of Blastocystis spp. ST 10 found in infected beef cattle in Kamal and Socah, Bangkalan, Madura, Indonesia.

Keywords: beef cattle, Blastocystis spp. subtype 10, Madura, Indonesia, zoonotic.