Molecular characteristic of Pasteurella multocida isolates from Sumba Island at East Nusa Tenggara Province, Indonesia

Research (Published online: 13-01-2020)

15. Molecular characteristic of Pasteurella multocida isolates from Sumba Island at East Nusa Tenggara Province, Indonesia

I. K. Narcana, I. W. Suardana and I. N. K. Besung

Veterinary World, 13(1): 104-109

I. K. Narcana: Master Student of Veterinary Medicine, Faculty of Veterinary Medicine, Udayana University, Jl. PB. Sudirman Denpasar-Bali, 80232, Indonesia.

I. W. Suardana: Department of Preventive Veterinary Medicine, Laboratory of Veterinary Public Health, Faculty of Veterinary Medicine, Udayana University, Jl. PB. Sudirman Denpasar-Bali, 80232, Indonesia.

I. N. K. Besung: Department of Pathobiology, Laboratory of Veterinary Microbiology, Faculty of Veterinary Medicine, Udayana University, Jl. PB. Sudirman Denpasar-Bali, 80232, Indonesia.

doi: www.doi.org/10.14202/vetworld.2020.104-109

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Article history: Received: 24-06-2019, Accepted: 25-11-2019, Published online: 13-01-2020

Corresponding author: I. W. Suardana

E-mail: wayan_suardana@unud.ac.id

Citation: Narcana IK, Suardana IW, Besung INK (2020) Molecular characteristic of Pasteurella multocida isolates from Sumba Island at East Nusa Tenggara Province, Indonesia, Veterinary World, 13(1): 104-109.

Abstract

Aim: This study aimed to determine the molecular characteristics of Pasteurella multocida isolates originated from Sumba Island, East Nusa Tenggara Province.

Materials and Methods: The isolates of P. multocida stored in frozen storage were cultured in blood agar as a selective medium and identified conventionally. Molecular tests were initiated by DNA isolation and then followed by polymerase chain reaction tests with specific primers for the determination of P. multocida serotype A or B. Positive strain of serotype B was then confirmed molecularly using 16S rRNA gene primer and followed by the sequencing of nucleotides.

Results: The study showed that both P. multocida isolates from Sumba island, i.e. PM1 is isolated from East Sumba district, while PM2 isolated from West Sumba district have 99.6% homology. Both isolates also known have 99% similarities with P. multocida originated from India, Britain, and Japan, respectively. The isolates share the same clade in the phylogenetic tree.

Conclusion: The 16S rRNA sequencing revealed a high similarity of P. multocida serotype B:2 isolated from Sumba island with the Indian isolates although the sample size is very small. Therefore, further molecular studies like multilocus sequence typing, VNTR need to be performed using a larger number of samples to establish the genetic relatedness observed in this study.

Keywords: genetic relatedness, molecular genetic, Pasteurella multocida, Septicemia Epizootica, Sumba island.