Open Access
Research (Published online: 24-05-2020)
22. Molecular characterization and phylogenetic analysis of fowl adenovirus serotype-4 from Guangdong Province, China
Fu Yuming, Yuan Sheng, Deng Wenyu, Chi Shihong, Li Wenfeng, Huang Wenjing, Li Xiaowen, Saeed El-Ashram, Kun Mei, Guo Jinyue, Zhang Xuelian, Li Zhili and Huang Shujian
Veterinary World, 13(5): 981-986

Fu Yuming: Department of Microbiology, College of Life Science and Engineering, Foshan University, Foshan 528231, Guangdong Province, China.
Yuan Sheng: Department of Microbiology, College of Life Science and Engineering, Foshan University, Foshan 528231, Guangdong Province, China.
Deng Wenyu: Guangdong Women and Children Hospital, Guangdong Province, China.
Chi Shihong: Department of Microbiology, College of Life Science and Engineering, Foshan University, Foshan 528231, Guangdong Province, China.
Li Wenfeng: Department of Microbiology, College of Life Science and Engineering, Foshan University, Foshan 528231, Guangdong Province, China.
Huang Wenjing: Department of Microbiology, College of Life Science and Engineering, Foshan University, Foshan 528231, Guangdong Province, China.
Li Xiaowen: Department of Microbiology, College of Life Science and Engineering, Foshan University, Foshan 528231, Guangdong Province, China.
Saeed El-Ashram: Department of Microbiology, College of Life Science and Engineering, Foshan University, Foshan 528231, Guangdong Province, China; Department of Zoology, Faculty of Science, Kafrelsheikh University, Kafr el-Sheikh 33516, Egypt.
Kun Mei: Department of Microbiology, College of Life Science and Engineering, Foshan University, Foshan 528231, Guangdong Province, China.
Guo Jinyue: Department of Microbiology, College of Life Science and Engineering, Foshan University, Foshan 528231, Guangdong Province, China.
Zhang Xuelian: Department of Microbiology, College of Life Science and Engineering, Foshan University, Foshan 528231, Guangdong Province, China.
Li Zhili: Department of Microbiology, College of Life Science and Engineering, Foshan University, Foshan 528231, Guangdong Province, China.
Huang Shujian: Department of Microbiology, College of Life Science and Engineering, Foshan University, Foshan 528231, Guangdong Province, China.

doi: www.doi.org/10.14202/vetworld.2020.981-986

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Article history: Received: 26-11-2019, Accepted: 09-04-2020, Published online: 24-05-2020

Corresponding author: Huang Shujian

E-mail: huangshujian@fosu.edu.cn

Citation: Yuming F, Sheng Y, Wenyu D, Shihong C, Wenfeng L, Wenjing H, Xiaowen L, El-Ashram S, Mei K, Jinyue G, Xuelian Z, Zhili L, Shujian H (2020) Molecular characterization and phylogenetic analysis of fowl adenovirus serotype-4 from Guangdong Province, China, Veterinary World, 13(5):981-986.
Abstract

Aim: Our aim in this study was to isolate potentially novel strains of fowl adenovirus serotype-4 (FAdV-4) that is currently circulating in broiler chicken flocks in Guangdong Province, China, and to compare nucleotide and amino acid (AA) sequences of their respective hexon genes.

Materials and Methods: The experiment was carried out on poultry farms experiencing outbreaks of FAdV-4-associated hydropericardium syndrome (HPS). Tissue samples from the hearts and livers of deceased chickens were screened for FAdV-4 infection using hexon gene-specific polymerase chain reaction (PCR).

Results: New virus isolates were used to infect 7-day-old chicks, which went onto reproduce typical HPS signs. The hypervariable region of the FAdV-4 hexon gene was PCR-amplified and sequenced. The hexon nucleotide and deduced AA sequence identities were 99.8-99.9% and 99.5-99.8%, respectively, among the four novel isolates. In addition, the new isolates were 97-100% and 96.4-99.9% identical to the nucleotide and deduced AA sequences, respectively, of FAdV-4 hexon genes available in the National Center for Biotechnology Information GenBank database. Phylogenetic analyses, based on the hexon gene sequence, revealed that the new isolates, clustered with FAdV-C; the FAdV-A, FAdV-B, FAdV-D, and FAdV-E viruses, were more distantly related.

Conclusion: New FAdV-4 isolates from Guangdong Province are similar to those identified in other regions of the world. This information provides critical insight into HPS epidemiology and provides a perspective for monitoring outbreaks and developing strategies for disease prevention.

Keywords: Angara disease, fowl adenovirus-4, phylogenetic analysis.