Open Access
Research (Published online: 31-05-2020)
26. Seroprevalence of African horse sickness in selected donkey populations in Namibia
Umberto Molini, Guendalina Zaccaria, Erick Kandiwa, Borden Mushonga, Siegfried Khaiseb, Charles Ntahonshikira, Bernard Chiwome, Ian Baines, Oscar Madzingira, Giovanni Savini and Nicola D'Alterio
Veterinary World, 13(5): 1005-1009

Umberto Molini: Department of Pathobiology, School of Veterinary Medicine, Faculty of Agriculture and Natural Resources, University of Namibia, Neudamm Campus, Namibia.
Guendalina Zaccaria: Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G. Caporale" 64100 Teramo, Italy.
Erick Kandiwa: Department of Pathobiology, School of Veterinary Medicine, Faculty of Agriculture and Natural Resources, University of Namibia, Neudamm Campus, Namibia.
Borden Mushonga: Department of Pathobiology, School of Veterinary Medicine, Faculty of Agriculture and Natural Resources, University of Namibia, Neudamm Campus, Namibia.
Siegfried Khaiseb: Department of Virology, Central Veterinary Laboratory, 24 Goethe Street, Windhoek, Namibia.
Charles Ntahonshikira: Department of Pathobiology, School of Veterinary Medicine, Faculty of Agriculture and Natural Resources, University of Namibia, Neudamm Campus, Namibia.
Bernard Chiwome: Department of Pathobiology, School of Veterinary Medicine, Faculty of Agriculture and Natural Resources, University of Namibia, Neudamm Campus, Namibia.
Ian Baines: Department of Pathobiology, School of Veterinary Medicine, Faculty of Agriculture and Natural Resources, University of Namibia, Neudamm Campus, Namibia.
Oscar Madzingira: Department of Pathobiology, School of Veterinary Medicine, Faculty of Agriculture and Natural Resources, University of Namibia, Neudamm Campus, Namibia.
Giovanni Savini: Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G. Caporale" 64100 Teramo, Italy.
Nicola D'Alterio: Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G. Caporale" 64100 Teramo, Italy.

doi: www.doi.org/10.14202/vetworld.2020.1005-1009

Share this article on [Facebook] [LinkedIn]

Article history: Received: 30-11-2019, Accepted: 20-04-2020, Published online: 31-05-2020

Corresponding author: Wichaporn Lerdweeraphon

E-mail: u.molini76@gmail.com

Citation: Molini U, Zaccaria G, Kandiwa E, Mushonga B, Khaiseb S, Ntahonshikira C, Chiwome B, Baines I, Madzingira O, Savini G, D'Alterio N (2020) Seroprevalence of African horse sickness in selected donkey populations in Namibia, Veterinary World, 13(5): 1005-1009.
Abstract

Background and Aim: African horse sickness (AHS) is a non-contagious viral disease of horses and other equids caused by an arbovirus belonging to the Reoviridae family and genus Orbivirus. AHS is an endemic disease that is responsible for the death of a high number of horses every year in Namibia. At present, there is no information on the prevalence and distribution of AHS virus (AHSV) serotypes in the different regions of Namibia. Therefore, this survey aimed to fill this knowledge gap by investigating the AHSV seroprevalence in Namibian donkeys.

Materials and Methods: A total of 260 blood samples (20 samples for each region) were randomly collected from donkeys aged between 3 and 5 years. Sera were screened for AHSV-specific immunoglobulin G antibodies using a commercial competitive enzyme-linked immunosorbent assay kit and samples positive to AHSV antibodies were further tested by serum neutralization (SN) assay to evaluate the AHSV serotype-specific immune response.

Results: Seroprevalence of antibodies against AHSV in Namibian donkeys was 63.5%. The AHSV prevalence was significantly higher in the northern region (64%) than in the southern region (36%). A significantly (p<0.05) higher number of donkeys had antibodies against AHSV-6 (37.8%) and AHSV-9 (37.8%). The AHSV-2, AHSV-6, and AHSV-9 prevalence were higher (p<0.05) in the northern regions compared to the southern regions. None of the donkeys in this study, however, tested positive for AHSV-8.

Conclusion: Results of the current study indicate that all AHSV serotypes have either circulated previously or are circulating in Namibia except for AHSV-8. In particular, AHSV-1, -2, -3, -4, -5, -6, and -9 serotypes have circulated or are circulating in the northern region of Namibia, while AHSV-1, -4, -5, -6, -7, and -9 have infected donkeys in the south. AHSV-9 and AHSV-6 were the most prevalent serotypes detected in donkeys in this study. SN results showed that several donkeys from Kavango East, Kavango West, and Ohangwena regions had been exposed to multiple serotypes, indicating the possibility of cocirculation of several strains in Namibia.

Keywords: African horse sickness, donkeys, enzyme-linked immunosorbent assay, Namibia.