Tilapia viscera hydrolysate extract alleviates oxidative stress and renal damage in deoxycorticosterone acetate-salt-induced hypertension rats

Research (Published online: 23-11-2020)

26. Tilapia viscera hydrolysate extract alleviates oxidative stress and renal damage in deoxycorticosterone acetate-salt-induced hypertension rats

Putut Har Riyadi, Mochammad Fitri Atho'illah, Wendy Alexander Tanod and Irma Sarita Rahmawati

Veterinary World, 13(11): 2477-2483

Putut Har Riyadi: Department of Fisheries Post Harvest Technology, Faculty of Fisheries and Marine Science, Diponegoro University, Semarang 1269, Central Java, Indonesia.

Mochammad Fitri Atho'illah: Department of Biology, Faculty of Mathematics and Natural Science, Brawijaya University, Malang 65145, East Java, Indonesia.

Wendy Alexander Tanod: Department of Fisheries Product Technology, Institute of Fisheries and Marine (Sekolah Tinggi Perikanan dan Kelautan), Palu 94118, Central Sulawesi, Indonesia; Department of Fisheries and Marine Science, Politeknik Negeri Nusa Utara, Tahuna 95821, North Sulawesi, Indonesia.

Irma Sarita Rahmawati: Department of Nutrition, Faculty of Medicine, Brawijaya University, Malang 65145, East Java, Indonesia.

doi: www.doi.org/10.14202/vetworld.2020.2477-2483

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Article history: Received: 07-06-2020, Accepted: 08-10-2020, Published online: 23-11-2020

Corresponding author: Putut Har Riyadi

E-mail: putut.riyadi@live.undip.ac.id

Citation: Riyadi PH, Atho'illah MF, Tanod WA, Rahmawati IS (2020) Tilapia viscera hydrolysate extract alleviates oxidative stress and renal damage in deoxycorticosterone acetate-salt-induced hypertension rats, Veterinary World, 13(11): 2477-2483.

Abstract

Background and Aim: Hypertension is closely related to oxidative stress conditions, which increases malondialdehyde (MDA) expression and renal damage. Tilapia viscera hydrolysate extract (TVHE) contains compounds and peptides that act as antioxidants. This study aimed to investigate TVHE therapy effect on MDA levels and renal histological conditions in deoxycorticosterone acetate (DOCA)-salt-induced hypertension rats.

Materials and Methods: Tilapia viscera were defatted and hydrolyzed using Alcalase enzyme to obtain TVHE. TVHE antioxidant activity was measured using the 1,1-diphenyl-2-picrylhydrazyl method. Fifteen Wistar male rats were divided into five groups: Normal control (without induced DOCA-salt), DOCA-salt, DOCA-salt+Captopril 5 mg/kg body weight (BW), DOCA-salt+TVHE 150 mg/kg BW, and DOCA-salt+TVHE 300 mg/kg BW. MDA level and renal histology were observed in each group.

Results: TVHE half maximal inhibitory concentration values ranged from 3.87±0.35 μg/mL to 42.03±3.55 μg/mL, which were identified as in the very strong Blois category. TVHE and captopril therapy reduced MDA expression significantly (p<0.05) compared to DOCA-salt only. TVHE and captopril therapy also improved glomerular damage in DOCA-salt-induced hypertension rats.

Conclusion: TVHE has antioxidant ability, decreased MDA level, and decreased glomerular damage in DOCA-salt-induced hypertension rats.

Keywords: antioxidant, hydrolysate, peptide, tilapia, viscera.