Background and Aim: Blood and serological parameters are indicators of the health status of the birds and influenced by the type of feed and their nutrient composition. Scanty researches are available in rice gluten meal (RGM) regarding its effect of feeding with and without enzymes on hematology and serum biochemistry. This study was conducted for in vitro and in vivo investigation regarding feeding RGM without or with different enzymes on hematology and serum biochemistry of broiler chickens. The in vitro study was done to determine chemical composition of RGM used in the biological trial.

Materials and Methods: A biological experiment with 384 broiler chicks was conducted to evaluate the effect of feeding RGM as soybean replacement without or with different enzymes on carcass characteristics in broiler chicken for 42 days. Two levels of RGM were taken (15% and 17.5%). Protease, xylanase, and multienzymes supplementation under different treatments were done.

Results: The RGM feeding and enzyme supplementation or their interaction revealed no significant (p>0.05) effects on the hematological parameters and serological parameters of broiler chickens except the significant (p<0.05) effect of enzyme supplementation on serum albumin and triglyceride values. The higher albumin values were observed in xylanase and protease supplemented birds and lower triglyceride values were observed in xylanase supplemented birds.

Conclusion: Thus, it may be concluded that RGM feeding at 15% or 17.5% inclusion level with or without enzymes had no adverse effect on hematobiochemical profile of broiler chickens.

Keywords: broiler, enzyme, hematology, rice gluten meal, serology.