Open Access
Research (Published online: 01-09-2020)
1. An immunohistochemical study of endocrine cells in the digestive tract of Varanus salvator (Reptile: Varanidae)
Mahfud Mahfud, Ernawati Ernawati, Nur R. Adawiyah Mahmud, Teguh Budipitojo and Hery Wijayanto
Veterinary World, 13(9): 1737-1742

Mahfud Mahfud: Department of Biology Education, University of Muhammadiyah Kupang, East of Nusa Tenggara Province, Indonesia.
Ernawati Ernawati: Department of Biology Education, University of Muhammadiyah Kupang, East of Nusa Tenggara Province, Indonesia.
Nur R. Adawiyah Mahmud: Department of Biology Education, University of Muhammadiyah Kupang, East of Nusa Tenggara Province, Indonesia.
Teguh Budipitojo: Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Gajah Mada University, Yogyakarta, Indonesia.
Hery Wijayanto: Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Gajah Mada University, Yogyakarta, Indonesia.

doi: www.doi.org/10.14202/vetworld.2020.1737-1742

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Article history: Received: 17-02-2020, Accepted: 10-07-2020, Published online: 01-09-2020

Corresponding author: Teguh Budipitojo

E-mail: budipitojo@ugm.ac.id

Citation: Mahfud M, Ernawati E, Mahmud NRA, Budipitojo T, Wijayanto H (2020) An immunohistochemical study of endocrine cells in the digestive tract of Varanus salvator (Reptile: Varanidae), Veterinary World, 13(9): 1737-1742.
Abstract

Aim: The aim of the study was to identify the distribution pattern and frequency of endocrine cell types in the digestive tract of Varanus salvator.

Materials and Methods: The presence of endocrine cells (glucagon, somatostatin, and serotonin) in the digestive tract (esophagus, stomach, and intestine) was detected using the avidin-biotin complex (ABC) method.

Results: Three types of endocrine cells immunoreactive to antisera glucagon, serotonin, and somatostatin were found in the caudal portion of the small and large intestines but were not observed in the esophagus, stomach, and caput and medial sections of the small intestine. Endocrine cells distributed in the digestive tract of V. salvator vary in color intensity, from weak to sharp, in response to the primer antibody.

Conclusion: Endocrine cells in the digestive tract that is immunoreactive to glucagon, somatostatin, and serotonin are those found in the caudal portion of the small and large intestines. They are varied in distribution pattern, frequency, and color intensity.

Keywords: digestive tract, endocrine cell, immunohistochemistry, Varanus salvator.