Effects of respiratory disease on Kele piglets lung microbiome, assessed through 16S rRNA sequencing

Research (Published online: 25-09-2020)

31. Effects of respiratory disease on Kele piglets lung microbiome, assessed through 16S rRNA sequencing

Jing Zhang, Kaizhi Shi, Jing Wang, Xiong Zhang, Chunping Zhao, Chunlin Du and Linxin Zhang

Veterinary World, 13(9): 1970-1981

Jing Zhang: Key Laboratory of Livestock and Poultry Major Epidemic Disease Monitoring and Prevention, Institute of Animal Husbandry and Veterinary Science, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou, China.

Kaizhi Shi: Key Laboratory of Livestock and Poultry Major Epidemic Disease Monitoring and Prevention, Institute of Animal Husbandry and Veterinary Science, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou, China.

Jing Wang: Key Laboratory of Livestock and Poultry Major Epidemic Disease Monitoring and Prevention, Institute of Animal Husbandry and Veterinary Science, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou, China.

Xiong Zhang: Key Laboratory of Livestock and Poultry Major Epidemic Disease Monitoring and Prevention, Institute of Animal Husbandry and Veterinary Science, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou, China.

Chunping Zhao: Key Laboratory of Livestock and Poultry Major Epidemic Disease Monitoring and Prevention, Institute of Animal Husbandry and Veterinary Science, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou, China.

Chunlin Du: Key Laboratory of Livestock and Poultry Major Epidemic Disease Monitoring and Prevention, Institute of Animal Husbandry and Veterinary Science, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou, China.

Linxin Zhang: Key Laboratory of Livestock and Poultry Major Epidemic Disease Monitoring and Prevention, Institute of Animal Husbandry and Veterinary Science, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou, China.

doi: www.doi.org/10.14202/vetworld.2020.1970-1981

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Article history: Received: 19-05-2020, Accepted: 07-08-2020, Published online: 25-09-2020

Corresponding author: Kaizhi Shi

E-mail: shkzjjp@163.com

Citation: Zhang J, Shi K, Wang J, Zhang X, Zhao C, Du C, Zhang L (2020) Effects of respiratory disease on Kele piglets lung microbiome, assessed through 16S rRNA sequencing, Veterinary World, 13(9): 1970-1981.

Abstract

Background and Aim: Due to the incomplete development of the immune system in immature piglets, the respiratory tract is susceptible to invasion by numerous pathogens that cause a range of potential respiratory diseases. However, few studies have reported the changes in pig lung microorganisms during respiratory infection. Therefore, we aimed to explore the differences in lung environmental microorganisms between healthy piglets and piglets with respiratory diseases.

Materials and Methods: Histopathological changes in lung sections were observed in both diseased and healthy pigs. Changes in the composition and abundance of microbiomes in alveolar lavage fluid from eleven 4-week-old Chinese Kele piglets (three clinically healthy and eight diseased) were studied by IonS5^TM XL sequencing of the bacterial 16S rRNA genes.

Results: Histopathological sections showed that diseased pigs displayed more lung lesions than healthy pigs. Diseased piglets harbored lower bacterial operational taxonomic units, α-diversity, and bacterial community complexity in comparison to healthy piglets. Taxonomic composition analysis showed that in the diseased piglets, the majority of flora was composed of Ureaplasma, Mycoplasma, and Actinobacillus; while Actinobacillus, Sphingomonas, and Stenotrophomonas were dominant in the control group. The abundance of Ureaplasma was significantly higher in ill piglets (p<0.05), and the phylogenetic tree indicated that Ureaplasma was clustered in Ureaplasma diversum, a conditional pathogen that has the potential to affect the swine respiratory system.

Conclusion: The results of this study show that the microbial species and structure of piglets' lungs were changed during respiratory tract infection. The finding of Ureaplasma suggested that besides known pathogens such as Mycoplasma and Actinobacillus, unknown pathogens can exist in the respiratory system of diseased pigs and provide a potential basis for clinical treatment.

Keywords: 16S rRNA sequencing, Kele piglets, microbial diversity, respiratory diseases, Ureaplasma, Ureaplasma diversum.