Open Access
Research (Published online: 22-04-2021)
22. Apoptosis-related gene expression induced by Colombian propolis samples in canine osteosarcoma cell line
Dolly Patricia Pardo-Mora, Oscar Julián Murillo, Mauricio Rey-Buitrago, Mónica Losada-Barragán, Jaime Fabian Cruz Uribe, Karina Basso Santiago, Bruno José Conti, Eliza de Oliveira Cardoso, Fernanda Lopes Conte, Rafael María Gutiérrez, Orlando Torres García and José Maurício Sforcin
Veterinary World, 14(4): 964-971

Dolly Patricia Pardo-Mora: Department of Animal Health, Facultad de Medicina Veterinaria, Universidad Antonio Nariño, Bogotá, Colombia.
Oscar Julián Murillo: Department of Morphology, Facultad de Medicina, Universidad Nacional de Colombia, Bogotá, Colombia.
Mauricio Rey-Buitrago: Department of Animal Health, Facultad de Medicina Veterinaria, Universidad Antonio Nariño, Bogotá, Colombia; Department of Morphology, Facultad de Medicina, Universidad Nacional de Colombia, Bogotá, Colombia.
Mónica Losada-Barragán: Department of Animal Health, Facultad de Medicina Veterinaria, Universidad Antonio Nariño, Bogotá, Colombia.
Jaime Fabian Cruz Uribe: Department of Animal Health, Facultad de Medicina Veterinaria, Universidad Antonio Nariño, Bogotá, Colombia.
Karina Basso Santiago: Department of Chemical and Biological Sciences, São Paulo State University (UNESP), Institute of Biosciences, Campus Botucatu, Brazil.
Bruno José Conti: Department of Chemical and Biological Sciences, São Paulo State University (UNESP), Institute of Biosciences, Campus Botucatu, Brazil.
Eliza de Oliveira Cardoso: Department of Chemical and Biological Sciences, São Paulo State University (UNESP), Institute of Biosciences, Campus Botucatu, Brazil.
Fernanda Lopes Conte: Department of Chemical and Biological Sciences, São Paulo State University (UNESP), Institute of Biosciences, Campus Botucatu, Brazil.
Rafael María Gutiérrez: Department of Animal Health, Facultad de Medicina Veterinaria, Universidad Antonio Nariño, Bogotá, Colombia.
Orlando Torres García: Department of Animal Health, Facultad de Medicina Veterinaria, Universidad Antonio Nariño, Bogotá, Colombia.
José Maurício Sforcin: Department of Chemical and Biological Sciences, São Paulo State University (UNESP), Institute of Biosciences, Campus Botucatu, Brazil.

doi: www.doi.org/10.14202/vetworld.2021.964-971

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Article history: Received: 08-10-2020, Accepted: 01-03-2021, Published online: 22-04-2021

Corresponding author: José Maurício Sforcin

E-mail: jose.m.sforcin@unesp.br

Citation: Pardo-Mora DP, Murillo OJ, Rey-Buitrago M, Losada-Barragán M, Uribe JFC, Santiago KB, Conti BJ, Cardoso EO, Conte FL, Gutiérrez RM, García OT, Sforcin JM (2021) Apoptosis-related gene expression induced by Colombian propolis samples in canine osteosarcoma cell line, Veterinary World, 14(4): 964-971.
Abstract

Background and Aim: Osteosarcoma (OSA) is the most common bone tumor in canines and humans. This study aimed to assess the cytotoxic and apoptotic effects of Colombian propolis samples on a canine OSA cell line (OSCA-8) by evaluating the expression of BCL-2, BAX, CASPASE 9, CASPASE 8, and TNFR1 genes involved in the apoptosis pathway.

Materials and Methods: After treating the cells with five Colombian propolis samples (Usm, Met, Fus, Sil, and Caj), we evaluated cell viability and lactate dehydrogenase (LDH) release. Early and late apoptosis was determined by flow cytometry using annexin V/propidium iodide. Furthermore, the effects of three selected samples on gene expression were analyzed by real-time polymerase chain reaction.

Results: The Colombian propolis samples reduced OSCA-8 cell viability and increased LDH release. All samples induced apoptosis significantly and upregulated BCL-2 and CASPASE 8 expression. Usm and Sil increased BAX expression, Met and Sil induced CASPASE 9 expression, and Usm increased TNFR1.

Conclusion: Colombian propolis samples exhibited cytotoxic and apoptotic effects on canine OSA cells, and CASPASE 8 upregulation indicated apoptosis induction by the extrinsic pathway.

Keywords: apoptosis, cytotoxicity, osteosarcoma, propolis.