Open Access
Research (Published online: 31-12-2021)
22. Melanocortin-4 receptor and leptin as genes for the selection of superior Madrasin cattle
Budi Utomo, Rimayanti Rimayanti, Indah Norma Triana and Amaq Fadholly
Veterinary World, 14(12): 3224-3228

Budi Utomo: Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, 60115, Indonesia.
Rimayanti Rimayanti: Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, 60115, Indonesia.
Indah Norma Triana: Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, 60115, Indonesia.
Amaq Fadholly: Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, 60115, Indonesia.

doi: www.doi.org/10.14202/vetworld.2021.3224-3228

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Article history: Received: 15-08-2021, Accepted: 24-11-2021, Published online: 31-12-2021

Corresponding author: Budi Utomo

E-mail: budi_reprovet@yahoo.com

Citation: Utomo B, Rimayanti R, Triana IN, Fadholly A (2021) Melanocortin-4 receptor and leptin as genes for the selection of superior Madrasin cattle, Veterinary World, 14(12): 3224-3228.
Abstract

Background and Aim: The genetic improvement of cattle through livestock section is based on quantitative, qualitative, and molecular characteristics. This study examined polymorphisms of the melanocortin-4 receptor (MC4R) and leptin genes as a reference for the selection of superior breeds in Madrasin cattle.

Materials and Methods: The leptin and MC4R genes of Madrasin cattle were amplified using polymerase chain reaction (PCR); then, restriction fragment length polymorphism of the leptin gene was performed using the restriction enzyme BsaA1, at site 2793 with ACGT point position.

Results: The leptin gene was divided into three bands, namely, AA with one fragment (522 bp), CG with two fragments (441 bp and 81 bp), and AG with three fragments (522 bp, 441 bp, and 81 bp). The MCR-4 gene was divided into three bands, namely, 493 bp, 318 bp, and 175 bp.

Conclusion: The MC4R and leptin genes can act as molecular markers for growth traits in Madrasin cattle and can be used to genetically optimize and improve growth. The GG allele of the MC4R gene and the AA allele of the leptin gene can be used in Madrasin cattle.

Keywords: leptin, Madrasin, melanocortin-4 receptor.