Research (Published online: 07-07-2021)
6. Molecular identification of fluoroquinolone resistance in Salmonella spp. isolated from broiler farms and human samples obtained from two regions in Colombia
María Paula Herrera-Sánchez, Rafael Enrique Castro-Vargas, Luz Clemencia Fandiño-de-Rubio, Roy Rodríguez-Hernández and Iang Schroniltgen Rondón-Barragán
Veterinary World, 14(7): 1767-1773
María Paula Herrera-Sánchez: Research Group in Immunobiology and Pathogenesis, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Santa Helena Highs, Ibagué 730006299, Tolima, Colombia.
Rafael Enrique Castro-Vargas: Research Group in Immunobiology and Pathogenesis, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Santa Helena Highs, Ibagué 730006299, Tolima, Colombia; Poultry Research Group, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Santa Helena Highs, Ibagué 730006299, Tolima, Colombia.
Luz Clemencia Fandiño-de-Rubio: Poultry Research Group, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Santa Helena Highs, Ibagué 730006299, Tolima, Colombia.
Roy Rodríguez-Hernández: Poultry Research Group, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Santa Helena Highs, Ibagué 730006299, Tolima, Colombia.
Iang Schroniltgen Rondón-Barragán: Research Group in Immunobiology and Pathogenesis, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Santa Helena Highs, Ibagué 730006299, Tolima, Colombia; Poultry Research Group, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Santa Helena Highs, Ibagué 730006299, Tolima, Colombia.
doi: www.doi.org/10.14202/vetworld.2021.1767-1773
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Article history: Received: 29-01-2021, Accepted: 24-05-2021, Published online: 07-07-2021
Corresponding author: Iang Schroniltgen Rondón-Barragán
E-mail: isrondon@ut.edu.co
Citation: Herrera- Sánchez MP, Castro-Vargas RE, Fandiño-de-Rubio LC, Rodríguez-Hernández R, Rondón-Barragán IS (2021) Molecular identification of fluoroquinolone resistance in Salmonella spp. isolated from broiler farms and human samples obtained from two regions in Colombia, Veterinary World, 14(7): 1767-1773.
Abstract
Background and Aim: Salmonella is one of the most common foodborne pathogens, the emergence of antibiotic-resistant strains of which is increasing. The aim of this study was to phenotypically and genotypically characterize the fluoroquinolone resistance of Salmonella isolates from broiler and humans in two regions of Colombia.
Materials and Methods: Salmonella strains (n=49) were evaluated. The phenotype of antibiotic resistance was assessed by an automated method and agar diffusion method, as well as the presence of the quinolone resistance genes qnrA, qnrB, qnrC, qnrD, qnrS, and aac(6')-Ib as determined by polymerase chain reaction.
Results: Strains were resistant to ciprofloxacin (75%), levofloxacin (57.1%), and enrofloxacin (38.8%). Molecular identification showed that 24 out of 49 strains possessed the qnrB gene (48.9%), while only one isolate from the Santander region possessed the aac(6')-Ib gene. Regarding Class 1 integron, it was present in 11 out of the 49 strains (22.44%).
Conclusion: This study reports the presence of the gene qnrB as well the presence of Class 1 integrons in broiler Salmonella isolates, which may contribute to the resistance to fluoroquinolones.
Keywords: antibiotic resistance, broiler, resistance genes, Salmonella.