Open Access
Research (Published online: 18-06-2021)
21. Comparative evaluation of cytokine and acute-phase protein concentrations in sera of dairy cows with subclinical and clinical ketosis as a different view of the causes of the disease
Piotr Brodzki, Jan Marczuk, Urszula Lisiecka, Marek Szczubiał, Adam Brodzki, Hubert Gorzkoś and Katarzyna Kulpa
Veterinary World, 14(6): 1572-1578

Piotr Brodzki: Department and Clinic of Animal Reproduction, Faculty of Veterinary Medicine, University of Life Science in Lublin, Lublin, Poland.
Jan Marczuk: Department and Clinic of Animal Internal Medicine, Faculty of Veterinary Medicine, University of Life Science in Lublin, Lublin, Poland.
Urszula Lisiecka: Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin, Poland.
Marek Szczubiał: Department and Clinic of Animal Reproduction, Faculty of Veterinary Medicine, University of Life Science in Lublin, Lublin, Poland.
Adam Brodzki: Department and Clinic of Animal Surgery, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin, Poland.
Hubert Gorzkoś: Private Veterinary Practitioner, Gorvet, Sztutowo, Poland.
Katarzyna Kulpa: Department and Clinic of Animal Reproduction, Faculty of Veterinary Medicine, University of Life Science in Lublin, Lublin, Poland.

doi: www.doi.org/10.14202/vetworld.2021.1572-1578

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Article history: Received: 31-12-2020, Accepted: 10-05-2021, Published online: 18-06-2021

Corresponding author: Piotr Brodzki

E-mail: wetdoc@interia.pl

Citation: Brodzki P, Marczuk J, Lisiecka U, Szczubiał M, Brodzki A, Gorzkoś H, Kulpa K (2021) Comparative evaluation of cytokine and acute-phase protein concentrations in sera of dairy cows with subclinical and clinical ketosis as a different view of the causes of the disease, Veterinary World, 14(6): 1572-1578.
Abstract

Background and Aim: Ketosis is a common disease occurring during the first stage of lactation in highly productive dairy cows. The aim of the present study was the comparative assessment of selected pro-inflammatory cytokines (including tumor necrosis factor-α [TNF-α] and interleukin 6 [IL-6]), anti-inflammatory cytokines (including IL-10), and acute-phase proteins (APPs) (including haptoglobin [Hp] and serum amyloid A [SAA]), in the sera of cows with subclinical ketosis (SCK), in those with clinical ketosis (CK), and in healthy cows.

Materials and Methods: Thirty dairy cows of Holstein-Friesian breed were investigated. The cows were divided into three groups depending on the serum β-hydroxybutyric acid (BHBA) level. The control, SCK, and CK groups included healthy cows, cows with SCK, and cows with CK, respectively. BHBA concentration in blood serum was determined using colorimetric method. The blood serum was used for proper tests. Cytokine (TNF-α, IL-6, and IL-10) and APPs (SAA and Hp) concentrations in the investigated samples were determined by enzyme-linked immunosorbent assay method.

Results: The SCK group had significantly higher TNF-α, IL-6; IL-10, and SAA values than had the CK group (p<0.01). The SCK group had a lower Hp concentration than had the CK group (p<0.05).

Conclusion: This study showed that the inflammation intensity is higher in the initial phase of the disease and decreases during the advancement, probably due to active anti-inflammatory mechanisms (an increase of IL-10 concentration), which protect animal organism from self-destruction. On the basis of our study, it can be assumed that ketosis development in dairy cows was preceded by the systemic inflammation that may influence the progress of this disease.

Keywords: acute-phase proteins, cytokine, dairy cows, ketosis.