Background and Aim: Hydrogen peroxide (H₂O₂) is a free radical, widely use as a food preservative, may cause adverse effects to the body. Mangosteen contains various antioxidants may scavenge free radical produced by H₂O₂. This study examined the effects of mangosteen peel infusion on the liver and kidney of rats after H₂O₂ induction.

Materials and Methods: Thirty-six female Wistar rats were divided into six groups: Negative control, positive control (1% H₂O₂), as well as mangosteen peel infusion groups at a concentration of 0.25; 0.5; 1, and 2%. H₂O₂ induction was administered orally every day for 2 months followed by mangosteen peel infusion treatment (1 month) after H₂O₂ induction for 1 month. At the end of the experiment, the rats were sacrificed. The liver and kidney of each rat were collected for reactive oxygen species (ROS) and histopathological examinations. Furthermore, blood was collected for creatinine examination.

Results: H₂O₂ induction caused the highest ROS level elevation in the positive control group which was treated with H₂O₂ only. Meanwhile, 2% of mangosteen peel infusion returned the ROS levels to normal. It was also observed that mangosteen peel infusion restored creatinine levels to normal. Furthermore, 2% of mangosteen peel infusion improved the histopathological structure of the liver and kidneys after H₂O₂ induction.

Conclusion: Mangosteen peel infusion especially at a concentration of 2% has the potential to improve liver and kidney structure and functions after H₂O₂ induction.

Keywords: antioxidant, female rat, H₂O₂, histological structure, mangosteen peel infusion.