Open Access
Research (Published online: 13-05-2021)
16. Identification and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus-associated subclinical mastitis isolated from dairy cows in Bogor, Indonesia
Evi Nur Qolbaini, Miftahudin Majid Khoeri, Korrie Salsabila, Wisiva Tofriska Paramaiswari, Wisnu Tafroji, I. Made Artika and Dodi Safari
Veterinary World, 14(5): 1180-1184

Evi Nur Qolbaini: Department of Biochemistry, IPB University, Jl. Raya Dramaga, Bogor, 16680, Indonesia.
Miftahudin Majid Khoeri: Eijkman Institute for Molecular Biology, Jl. Diponegoro No. 69 Jakarta, 10340, Indonesia.
Korrie Salsabila: Eijkman Institute for Molecular Biology, Jl. Diponegoro No. 69 Jakarta, 10340, Indonesia.
Wisiva Tofriska Paramaiswari: Eijkman Institute for Molecular Biology, Jl. Diponegoro No. 69 Jakarta, 10340, Indonesia.
Wisnu Tafroji: Eijkman Institute for Molecular Biology, Jl. Diponegoro No. 69 Jakarta, 10340, Indonesia.
I. Made Artika: Department of Biochemistry, IPB University, Jl. Raya Dramaga, Bogor, 16680, Indonesia; Eijkman Institute for Molecular Biology, Jl. Diponegoro No. 69 Jakarta, 10340, Indonesia.
Dodi Safari: Eijkman Institute for Molecular Biology, Jl. Diponegoro No. 69 Jakarta, 10340, Indonesia.

doi: www.doi.org/10.14202/vetworld.2021.1180-1184

Share this article on [Facebook] [LinkedIn]

Article history: Received: 24-10-2020, Accepted: 30-03-2021, Published online: 13-05-2021

Corresponding author: Dodi Safari

E-mail: safari@eijkman.go.id

Citation: Qolbaini EN, Khoeri MM, Salsabila K, Paramaiswari WT, Tafroji W, Artika IM, Safari D (2021) Identification and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus-associated subclinical mastitis isolated from dairy cows in Bogor, Indonesia, Veterinary World, 14(5): 1180-1184.
Abstract

Background and Aim: Subclinical mastitis is an udder infection devoid of clinical symptoms, and Staphylococcus aureus is one of the bacteria causing this disease. This study aimed to identify and determine the prevalence and antibiotic susceptibility of methicillin-resistant S. aureus (MRSA)-associated subclinical mastitis isolated from dairy cows in Bogor, Indonesia.

Materials and Methods: S. aureus was isolated from subclinical mastitis milk specimens. All strains were confirmed by polymerase chain reaction-based detection of staphylococcal nuc, and MRSA was confirmed by the presence of mecA. Antibiotic susceptibility was determined using the disk diffusion method.

Results: From 86 milk samples, 49 isolates (57%) were confirmed as S. aureus. All S. aureus isolates were susceptible to tetracycline, gentamicin, chloramphenicol, erythromycin, and trimethoprim/sulfamethoxazole. Nine isolates were identified as MRSA (10.5%).

Conclusion: In this study, we reported MRSA-associated subclinical mastitis in Bogor, Indonesia.

Keywords: Bogor, dairy cows, methicillin-resistant Staphylococcus aureus, subclinical mastitis.