Genetic variation in the first intron and exon of the myostatin gene in several Indonesian cattle populations

Research (Published online: 19-05-2021)

18. Genetic variation in the first intron and exon of the myostatin gene in several Indonesian cattle populations

Peni Wahyu Prihandini, Almira Primasari, Aryogi Aryogi, Jauhari Efendy, Muchamad Luthfi, Dicky Pamungkas and Dwi Nur Happy Hariyono

Veterinary World, 14(5): 1197-1201

Peni Wahyu Prihandini: Beef Cattle Research Institute of Grati, Pasuruan, Indonesia.

Almira Primasari: Beef Cattle Research Institute of Grati, Pasuruan, Indonesia.

Aryogi Aryogi: Beef Cattle Research Institute of Grati, Pasuruan, Indonesia.

Jauhari Efendy: Beef Cattle Research Institute of Grati, Pasuruan, Indonesia.

Muchamad Luthfi: Beef Cattle Research Institute of Grati, Pasuruan, Indonesia.

Dicky Pamungkas: Beef Cattle Research Institute of Grati, Pasuruan, Indonesia.

Dwi Nur Happy Hariyono: Department of Animal Science, Faculty of Agriculture, Universitas Khairun, Ternate, Indonesia.

doi: www.doi.org/10.14202/vetworld.2021.1197-1201

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Article history: Received: 11-12-2020, Accepted: 30-03-2021, Published online: 19-05-2021

Corresponding author: Peni Wahyu Prihandini

E-mail: peniprihandini@pertanian.go.id

Citation: Prihandini PW, Primasari A, Aryogi A, Efendy J, Luthfi M, Pamungkas D, Hariyono DNH (2021) Genetic variation in the first intron and exon of the myostatin gene in several Indonesian cattle populations, Veterinary World, 14(5): 1197-1201.

Abstract

Background and Aim: Myostatin (MSTN), a member of the transforming growth factor-β family, is a negative regulator of muscle mass. This study aimed to detect the genetic variation of the 1160 bp fragment of exon 1 and part of intron 1 of the MSTN gene in several cattle populations raised in Indonesia.

Materials and Methods: Polymerase chain reaction products of the MSTN gene amplified from 92 animals representing 10 cattle populations (Peranakan Ongole [PO], Belgian Blue x PO cross, Rambon, PO x Bali cross, Jabres, Galekan, Sragen, Donggala, Madura, and Bali) were sequenced, compared, and aligned with bovine MSTN of Bos taurus (GenBank Acc. No. AF320998.1) and Bos indicus (GenBank Acc. No. AY794986.1).

Results: Four nucleotide substitutions (nt 1045 and 1066 in intron 1; nt 262 and 418 in exon 1) and two indels (nt 807 and 869 in intron 1) were synonymous mutations. Among these substitutions, only the nt 262G>C and nt 418A>G loci were polymorphic in all populations, except Bali cattle. The frequencies of the nt 262C (0.82) and nt 418A (0.65) alleles were highest. For the nt 262G>C locus, the CC genotype had the highest frequency (0.66) followed by GC (0.30) and CC (0.03). For the nt 418A>G locus, the AG genotype had the highest frequency (0.52) followed by AA (0.39) and GG (0.09).

Conclusion: The results, showing genetic variations in exon 1 and intron 1 of the MSTN gene, might be helpful for future association studies.

Keywords: beef cattle, double muscling, myostatin gene, polymorphisms, single-nucleotide polymorphism.