Determination of extended-spectrum β-lactamase-producing Klebsiella pneumoniae isolated from horses with respiratory manifestation

Research (Published online: 06-04-2022)

2. Determination of extended-spectrum β-lactamase-producing Klebsiella pneumoniae isolated from horses with respiratory manifestation

Amany A. Arafa, Riham H. Hedia, Sohad M. Dorgham, Eman S. Ibrahim, Magdy A. Bakry, Abeer M. Abdalhamed and Azza S. M. Abuelnaga

Veterinary World, 15(4): 827-833

Amany A. Arafa: Department of Microbiology and Immunology, National Research Centre, Dokki, Egypt.

Riham H. Hedia: Department of Microbiology and Immunology, National Research Centre, Dokki, Egypt.

Sohad M. Dorgham: Department of Microbiology and Immunology, National Research Centre, Dokki, Egypt.

Eman S. Ibrahim: Department of Microbiology and Immunology, National Research Centre, Dokki, Egypt.

Magdy A. Bakry: Department of Microbiology and Immunology, National Research Centre, Dokki, Egypt.

Abeer M. Abdalhamed: Department of Parasitology and Animal Diseases, National Research Centre, Dokki, Egypt.

Azza S. M. Abuelnaga: Department of Microbiology and Immunology, National Research Centre, Dokki, Egypt.

doi: www.doi.org/10.14202/vetworld.2022.827-833

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Article history: Received: 08-12-2021, Accepted: 28-02-2022, Published online: 06-04-2022

Corresponding author: Amany A. Arafa

E-mail: dr.amanyahmed@yahoo.com

Citation: Arafa AA, Hedia RH, Dorgham SM, Ibrahim ES, Bakry MA, Abdalhamed AM, Abuelnaga ASM (2022) Determination of extended-spectrum β-lactamase-producing Klebsiella pneumoniae isolated from horses with respiratory manifestation, Veterinary World, 15(4): 827-833.

Abstract

Background and Aim: The World Health Organization considers multidrug-resistant (MDR) Klebsiella pneumoniae a major global threat. Horses harbor commensal isolates of this bacterial species and potentially serve as reservoirs for human MDR bacteria. This study investigated antimicrobial resistance in horses caused by extended-spectrum β-lactamase (ESBL)-producing K. pneumoniae.

Materials and Methods: One hundred fifty-nine nasal swab samples were collected from horses with respiratory distress not treated with cefotaxime and erythromycin. Biochemical and serological identification was performed on all samples. Polymerase chain reaction (PCR) was used to detect 16S-23S ITS, mucoviscosity-associated gene (magA), uridine diphosphate galacturonate 4-epimerase gene (uge), and iron uptake system gene (kfu), bla_TEM, bla_SHV, and bla_CTX genes. Sequence analysis and phylogenetic relatedness of randomly selected K. pneumoniae isolates carrying the bla_TEM gene were performed.

Results: Ten isolates of Klebsiella spp. were obtained from 159 samples, with an incidence of 6.28% (10 of 159). Based on biochemical and serological identification, K. pneumoniae was detected in 4.4% (7 of 159) of the samples. Using PCR, all tested K. pneumoniae isolates (n=7) carried the 16S-23S ITS gene. By contrast, no isolates carried magA, uge, and kfu genes. The bla_TEM gene was detected in all test isolates. Moreover, all isolates did not harbor the bla_SHV or bla_CTX gene. Sequence analysis and phylogenetic relatedness reported that the maximum likelihood unrooted tree generated indicated the clustering of the test isolate with the other Gram-negative isolate bla_TEM. Finally, the sequence distance of the bla_TEM gene of the test isolate (generated by Lasergene) showed an identity range of 98.4-100% with the bla_TEM gene of the different test isolates.

Conclusion: The misuse of antimicrobials and insufficient veterinary services might help generate a population of ESBL-producing K. pneumoniae in equines and humans, representing a public health risk.

Keywords: extended-spectrum β-lactamase, horse, Klebsiella pneumoniae, multidrug-resistant, respiratory manifestation.