Open Access
Research (Published online: 11-04-2022)
9. Pro-inflammatory cytokine release from chicken peripheral blood mononuclear cells stimulated with lipopolysaccharide
Jatuporn Rattanasrisomporn, Chananphat Tantikositruj, Anyarat Thiptara, Warangkana Kitpipit, Ittidet Wichianrat, Autchara Kayan and Chaiwat Boonkaewwan
Veterinary World, 15(4): 885-889

Jatuporn Rattanasrisomporn: Department of Companion Animal Clinical Science, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
Chananphat Tantikositruj: Department of Animal Science, Kasetsart University, Bangkok 10900, Thailand.
Anyarat Thiptara: Department of Livestock Development, Veterinary Research and Development Center (Upper Southern Region), Nakhon Si Thammarat 80110, Thailand.
Warangkana Kitpipit: Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand; One Health Research Center, Walailak University, Nakhon Si Thammarat 80160, Thailand. Food Technology and Innovation Center of Excellence, Walailak University, Nakhon Si Thammarat, 80160, Thailand.
Ittidet Wichianrat: Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand.
Autchara Kayan: Department of Animal Science, Kasetsart University, Bangkok 10900, Thailand.
Chaiwat Boonkaewwan: Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand; One Health Research Center, Walailak University, Nakhon Si Thammarat 80160, Thailand.

doi: www.doi.org/10.14202/vetworld.2022.885-889

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Article history: Received: 30-10-2021, Accepted: 15-02-2022, Published online: 11-04-2022

Corresponding author: Chaiwat Boonkaewwan

E-mail: chaiwat.bo@wu.ac.th

Citation: Rattanasrisomporn J, Tantikositruj C, Thiptara A, Kitpipit W, Wichianrat I, Kayan A, Boonkaewwan C (2022) Pro-inflammatory cytokine release from chicken peripheral blood mononuclear cells stimulated with lipopolysaccharide, Veterinary World, 15(4): 885-889.
Abstract

Background and Aim: The principal cytokines released by the host on infection include pro-inflammatory cytokines such as interleukin (IL)-1, IL-6, and tumor necrosis factor (TNF). These cytokines were regarded as regulators of the host's response to infection. This study aimed to determine the release of pro-inflammatory cytokines from chicken peripheral blood mononuclear cells (PBMCs) following lipopolysaccharide (LPS) stimulation.

Materials and Methods: Blood samples were collected from six Betong chickens. To isolate PBMCs, density gradient centrifugation was utilized. PBMC culture in RPMI1640 with 10% fetal bovine serum was stimulated with various concentrations of LPS (0, 0.01, 0.1, and 1.0 μg/mL). The production of TNF-α, IL-1β, and IL-6 was determined using an enzyme-linked immunosorbent assay.

Results: When the PBMCs were cultured for 24 h with varying doses of LPS, there was no significant variation in cell viability. TNF-α, IL-1β, and IL-6 levels were measured in Betong chicken PBMC. The release of these cytokines increased considerably as LPS concentration (0.01-1 μg/mL) increased (p<0.05).

Conclusion: In vitro studies of the chicken immune response, notably the release of pro-inflammatory cytokines, can be conducted using PBMCs obtained from chicken blood.

Keywords: chicken, interleukin-1β, interleukin-6, peripheral blood mononuclear cell, tumor necrosis factor-α.