Diversity of mosquito species and potential arbovirus transmission in long-tailed macaque (Macaca fascicularis) breeding facilities

Research (Published online: 18-08-2022)

8. Diversity of mosquito species and potential arbovirus transmission in long-tailed macaque (Macaca fascicularis) breeding facilities

Dimas Novianto, Upik Kesumawati Hadi, Susi Soviana, Supriyono Supriyono, Lis Rosmanah, and Huda Shalahudin Darusman

Veterinary World, 15(8): 1961-1968

Dimas Novianto: Parasitology and Medical Entomology Laboratory, Animal Biomedicine Study Program, Graduate School, IPB University, Bogor, Indonesia.

Upik Kesumawati Hadi: Department of Animal Infectious Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University, Bogor, Indonesia.

Susi Soviana: Department of Animal Infectious Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University, Bogor, Indonesia.

Supriyono Supriyono: Department of Animal Infectious Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University, Bogor, Indonesia.

Lis Rosmanah: Primate Research Centre, Institute of Research and Community Service IPB University, Bogor, Indonesia.

Huda Shalahudin Darusman: Primate Research Centre, Institute of Research and Community Service IPB University, Bogor, Indonesia; Department of Anatomy, Physiology and Pharmacology, Faculty of Veterinary Medicine, IPB University, Bogor, Indonesia.

doi: www.doi.org/10.14202/vetworld.2022.1961-1968

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Article history: Received: 07-03-2022, Accepted: 29-06-2022, Published online: 18-08-2022

Corresponding author: Upik Kesumawati Hadi

E-mail: upikke@apps.ipb.ac.id

Citation: Novianto D, Hadi UK, Soviana S, Supriyono S, Rosmanah L, and Darusman HS (2022) Diversity of mosquito species and potential arbovirus transmission in long-tailed macaque (Macaca fascicularis) breeding facilities, Veterinary World, 15(8): 1961–1968.

Abstract

Background and Aim: Mosquito-borne viral infections are diseases that reduce human and animal health levels. Their transmission involves wildlife animals as reservoirs and amplifying hosts, including long-tailed macaques (Macaca fascicularis), and potentially transmits to humans and vice versa. This study aimed to determine the species diversity, richness, and biting activity of mosquitoes in a long-tailed macaque breeding area facility and discover the presence of Flavivirus and Alphavirus as the two main arboviruses reported to infect macaques.

Materials and Methods: Human landing catch, light trap, and sweep net methods were used for mosquito collection around long-tailed macaques cages at parallel times for 12 h (18:00–06:00) for 12 nights. Mosquito species were identified to the species level based on the morphological identification key for Indonesian mosquitoes. Mosquito diversity was analyzed by several diversity indices. Mosquitoes caught using the human landing catch method were pooled based on mosquito species for viral ribonucleic acid extraction. Reverse transcription-polymerase chain reaction (RT-PCR) detected the non-structural protein 5 of the Flavivirus region and the non-structural protein 4 of the Alphavirus region. This study used the man-hour density and man-biting rate formulas for mosquito density.

Results: Ten mosquito species were collected, namely, Aedes albopictus, Anopheles aconitus, Anopheles minimus, Anopheles vagus, Armigeres foliatus, Armigeres subalbatus, Culex gelidus, Culex hutchinsoni, Culex tritaeniorhynchus, and Culex quinquefasciatus. The number of mosquitoes caught using the light trap method had the highest abundance. In contrast, the number of mosquito species caught using the sweep net method had lower diversity than the other two methods. Seven mosquito species were obtained using the human landing catch method. The mosquito species with the highest density was Cx. quinquefasciatus within the observed densest period from 20:00 to 21:00. Negative results were obtained from RT-PCR testing on five species detected using universal Flavivirus and Alphavirus primers.

Conclusion: The occurrence of mosquitoes in long-tailed macaque breeding facilities can be a source of transmission of zoonotic vector-borne diseases between animals and humans and vice versa.

Keywords: arbovirus, diversity, ecology, health, primates.