Cervical anticancer activities of Annona squamosa Linn. leaf isolate

Research (Published online: 24-01-2022)

16. Cervical anticancer activities of Annona squamosa Linn. leaf isolate

Made Dira Swantara, Wiwik Susanah Rita, Made Asmarani Dira and Kadek Karang Agustina

Veterinary World, 15(1): 124-131

Made Dira Swantara: Department of Applied Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University, Denpasar, Bali 80225 Indonesia.

Wiwik Susanah Rita: Department of Applied Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University, Denpasar, Bali 80225 Indonesia.

Made Asmarani Dira: Clinical and Community Pharmacy Study Program, Faculty of Health, Bali Institute of Technology and Health, Denpasar, Bali 80225, Indonesia.

Kadek Karang Agustina: Department of Public Health, The Faculty of Veterinary Medicine, Udayana University, Denpasar, Bali 80225, Indonesia.

doi: www.doi.org/10.14202/vetworld.2022.124-131

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Article history: Received: 16-09-2021, Accepted: 20-12-2021, Published online: 24-01-2022

Corresponding authors: Made Dira Swantara

E-mail: dira_swantara@unud.ac.id

Citation: Swantara MD, Rita WS, Dira MA, Agustina KK (2022) Cervical anticancer activities of Annona squamosa Linn. leaf isolate, Veterinary World, 15(1): 124-131.

Abstract

Background and Aim: Cancer is one of the leading causes of death, the need for new anticancer herbal drugs is becoming more urgent considering the side effects of synthetic drugs. This study aimed to determine the anticancer activity of isolates derived from the methanol extract of Annona squamosa Linn. leaves and to identify the compounds that have an active effect against HeLa cells.

Materials and Methods: The leaf metabolites of A. squamosa L. were extracted using methanol at room temperature (28°C) and were partitioned into n-hexane, chloroform, and n-butanol. The toxicity test of these extracts was conducted using a brine shrimp lethality assay. Furthermore, the most toxic extracts were separated and purified using silica gel column chromatography to yield four isolate fractions: FA, FB, FC, and FD. The most toxic isolates were tested for anticancer against HeLa cells, and their compounds were identified using liquid chromatography-mass spectrometry.

Results: The results showed that the most toxic isolate with an LC₅₀ value of 100.00 ppm had a potency similar to that of an anticancer agent with an IC₅₀ value of 70.9021 ppm. Furthermore, the five compounds identified in this isolate include (6S, 7aR)-6-hydroxy-4,4,7a-trimethyl-6,7-dihydro-5H-1-benzofuran-2-one or loliolide, cocamidopropyl betaine, N-[3- (dimethylamino)propyl]dodecanamide or lauramidopropyl dimethylamine, linolenic acid, and 1-dodecyl-2-azepanone or laurocapram.

Conclusion: It can be concluded that the leaf isolates of A. squamosa Linn. had shown anticancer activities against cervical cancer.

Keywords: Annona squamosa Linn, cervical anticancer activities, HeLa cells.