Open Access
Research (Published online: 28-01-2022)
23. The improvement of insulin level after hydrogen-rich water therapy in streptozotocin-induced diabetic rats
Ekowati Retnaningtyas, Budi Susatia, Siti Nur Arifah and Sri Rahayu Lestari
Veterinary World, 15(1): 182-187

Ekowati Retnaningtyas: Department of Nursing, Politeknik Kesehatan Kemenkes Malang, Jl. Besar Ijen No. 77C, Malang 65119, East Java, Indonesia.
Budi Susatia: Department of Nursing, Politeknik Kesehatan Kemenkes Malang, Jl. Besar Ijen No. 77C, Malang 65119, East Java, Indonesia.
Siti Nur Arifah: Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang (State University of Malang), Jl. Semarang No. 5, Malang 65145, East Java, Indonesia.
Sri Rahayu Lestari: Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang (State University of Malang), Jl. Semarang No. 5, Malang 65145, East Java, Indonesia.

doi: www.doi.org/10.14202/vetworld.2022.182-187

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Article history: Received: 29-09-2021, Accepted: 28-12-2021, Published online: 28-01-2022

Corresponding authors: Ekowati Retnaningtyas

E-mail: ekowati_retnaningtyas@poltekkes-malang.ac.id

Citation: Retnaningtyas E, Susatia B, Arifah SN, Lestari SR (2022) The improvement of insulin level after hydrogen-rich water therapy in streptozotocin-induced diabetic rats, Veterinary World, 15(1): 182-187.
Abstract

Background and Aim: Water plays a pivotal role in the body. Alteration of the fluid balance promotes metabolic disorder, thus leading to the development of various diseases, such as diabetes mellitus (DM). Hydrogen-rich water (HW) is recognized as a novel antioxidant. This study aimed to investigate the role of HW on insulin, insulin receptor (IRs), and superoxide dismutase (SOD) levels in streptozotocin (STZ)-induced diabetic rats.

Materials and Methods: A total of 30 male Wistar rats were randomly divided into five groups: Normal (N), DM rats, DM+metformin (DM+Met, 45 mg/kg body weight [BW]), DM+Met+HW, and DM+HW. DM rats were induced by feeding them a high-fat diet for 30 days and then injecting with repeated low doses of STZ (35 mg/kg BW) intraperitoneally. Fresh HW was administered orally and ad libitum for 14 days. Insulin, IRs, and SOD were observed in each group.

Results: HW therapy increased the level and expression of insulin and IRs. In addition, treatment with HW also elevated the SOD levels in the serum and liver. The study results indicated no significant differences between the administration of HW and metformin.

Conclusion: HW has antioxidant activity in STZ-induced DM rats, increasing insulin, IRs, and SOD.

Keywords: diabetes mellitus, hydrogen-rich water, insulin receptor, insulin, superoxide dismutase.