First report of Avian metapneumovirus type B in Iraqi broiler flocks with swollen head syndrome

Research (Published online: 08-01-2022)

3. First report of Avian metapneumovirus type B in Iraqi broiler flocks with swollen head syndrome

Baraa Akeel Al-Hasan, Abdullah O. Alhatami, Husam Muhsen Abdulwahab, Ghadeer Sabah Bustani, Muhammad Ali Hameed and Ameer Haider Jawad

Veterinary World, 15(1): 16-21

Baraa Akeel Al-Hasan: Department of Medical Laboratory Technology, College of Medical Technology, The Islamic University, Najaf, Iraq.

Abdullah O. Alhatami: Department of Microbiology, Faculty of Veterinary Medicine, University of Kufa, Najaf, Iraq.

Husam Muhsen Abdulwahab: Department of Pathology, Faculty of Veterinary Medicine, University of Kufa, Najaf, Iraq.

Ghadeer Sabah Bustani: Department of Physiology and Pharmacology, The Islamic University, Najaf, Iraq; Department of Physiology and Pharmacology, College of Nursing, Altoosi University College, Najaf, Iraq.

Muhammad Ali Hameed: Department of Medical Laboratory Technology, College of Medical Technology, The Islamic University, Najaf, Iraq.

Ameer Haider Jawad: Department of Medical Laboratory Technology, College of Medical Technology, The Islamic University, Najaf, Iraq.

doi: www.doi.org/10.14202/vetworld.2022.16-21

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Article history: Received: 12-08-2021, Accepted: 22-11-2021, Published online: 08-01-2022

Corresponding author: Ghadeer Sabah Bustani

E-mail: bustani@iunajaf.edu.iq

Citation: Al-Hasan BA, Alhatami AO, Abdulwahab HM, Bustani GS, Hameed MA, Jawad AH (2022) First report of Avian metapneumovirus type B in Iraqi broiler flocks with swollen head syndrome, Veterinary World, 15(1): 16-21.

Abstract

Background and Aim: Swollen head syndrome (SHS) is a complex disease caused by various agents, including bacterial and viral pathogens, as well as environmental factors. Avian metapneumovirus (aMPV) is one of the most important causes of respiratory diseases and SHS in poultry and one of the most widespread viruses worldwide; however, it has not been recorded in Iraq. This study aimed at the molecular identification and subtyping of aMPV in poultry, with the objectives of investigating the prevalence of aMPV in infected broiler flocks with SHS and molecular typing using primers specific to the study of the prevalence of subtypes A, B, and C of aMPV.

Materials and Methods: This study was performed on 67 broiler farms that reported typical SHS from September 2018 to August 2019. Swabs were collected from the trachea, infraorbital sinuses, and lung, then uploaded on FTA cards and subjected to an RNA extraction protocol.

Results: aMPV was detected in 16 (23.8%) samples. Molecular typing using primers specific to the attachment glycoprotein (G) gene showed that all positive samples belonged to subtype B, as assessed using the real-time polymerase chain reaction technique.

Conclusion: aMPV may be the main etiological factor causing SHS in poultry. Moreover, this was the first report of the prevalence of subtype B aMPV strains in broiler farms in Iraq.

Keywords: Avian metapneumovirus type (B), Iraq, middle Euphrates region, swollen head syndrome, upper respiratory tract infection, viral infection poultry.