Genetic characterization and distribution of the virus in chicken embryo tissues infected with Newcastle disease virus isolated from commercial and native chickens in Indonesia

Research (Published online: 14-06-2022)

9. Genetic characterization and distribution of the virus in chicken embryo tissues infected with Newcastle disease virus isolated from commercial and native chickens in Indonesia

Liza Angeliya, Yuli Purwandari Kristianingrum, Widya Asmara and Michael Haryadi Wibowo

Veterinary World, 15(6): 1467-1480

Liza Angeliya: Veterinary Science Doctoral Study Program, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia; Disease Investigation Center Lampung, Jalan Untung Suropati Bandar Lampung, Lampung, 35142, Indonesia.

Yuli Purwandari Kristianingrum: Department of Pathology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia.

Widya Asmara: Department of Microbiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia.

Michael Haryadi Wibowo: Department of Microbiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia.

doi: www.doi.org/10.14202/vetworld.2022.1467-1480

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Article history: Received: 17-01-2022, Accepted: 22-04-2022, Published online: 14-06-2022

Corresponding author: Michael Haryadi Wibowo

E-mail: mhwibowo@ugm.ac.id

Citation: Angeliya L, Kristianingrum YP, Asmara W, Wibowo MH (2022) Genetic characterization and distribution of the virus in chicken embryo tissue infected with Newcastle disease virus isolated from commercial and native chickens in Indonesia, Veterinary World, 15(6): 1467-1480.

Abstract

Background and Aim: Newcastle disease (ND) is a viral infectious disease that affects commercial and native chickens, resulting in economic losses to the poultry industry. This study aimed to examine the viral strains circulating in commercial and native chickens by genetic characterization and observe the distribution of Newcastle disease virus (NDV) in chicken embryonic tissue.

Materials and Methods: ND was detected using a quantitative reverse transcription-polymerase chain reaction. Genetic characterization of the fusion (F) and hemagglutinin-neuraminidase (HN) genes from the eight NDVs was performed using specific primers. The sequence was compared with that of other NDVs from GenBank and analyzed using the MEGA-X software. The distribution of NDV in chicken embryos was analyzed based on lesions and the immunopositivity in immunohistochemistry staining.

Results: Based on F gene characterization, velogenic NDV strains circulating in commercial and native chickens that showed varying clinical symptoms belonged to genotype VII.2. Lentogenic strains found in chickens without clinical symptoms were grouped into genotype II (unvaccinated native chickens) and genotype I (vaccinated commercial chickens). Amino acid variations in the HN gene, namely, the neutralization epitope and antigenic sites at positions 263 and 494, respectively, occurred in lentogenic strains. The NDV reaches the digestive and respiratory organs, but in lentogenic NDV does not cause significant damage, and hence embryo death does not occur.

Conclusion: This study showed that velogenic and lentogenic NDV strains circulated in both commercial and native chickens with varying genotypes. The virus was distributed in almost all organs, especially digestive and respiratory. Organ damage in lentogenic infection is not as severe as in velogenic NDV. Further research is needed to observe the distribution of NDV with varying pathogenicity in chickens.

Keywords: fusion, gene characterization, hemagglutinin-neuraminidase, Newcastle disease, pathogenicity, viral distribution.