Occurrence of antimicrobial resistance and antimicrobial resistance genes in methicillin-resistant Staphylococcus aureus isolated from healthy rabbits

Research (Published online: 27-11-2022)

22. Occurrence of antimicrobial resistance and antimicrobial resistance genes in methicillin-resistant Staphylococcus aureus isolated from healthy rabbits

Arunee Jangsangthong, Nawarat Suriyakhun, Witawat Tunyong, Thida Kong-Ngoen, Sirijan Santajit, Nitaya Indrawattana, and Shutipen Buranasinsup

Veterinary World, 15(11): 2699-2704

Arunee Jangsangthong: Department of Pre-clinic and Applied Animal Science, Faculty of Veterinary Science, Mahidol University, Nakornpathom 73710, Thailand.

Nawarat Suriyakhun: Prasu-Arthorn Animal Hospital, Faculty of Veterinary Science, Mahidol University, Nakhonpathom 73170, Thailand.

Witawat Tunyong: Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand.

Thida Kong-Ngoen: Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand.

Sirijan Santajit: Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand; Research Center in Tropical Pathobiology, Walailak University, Nakhon Si Thammarat 80160, Thailand.

Nitaya Indrawattana: Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand.

Shutipen Buranasinsup: Department of Pre-clinic and Applied Animal Science, Faculty of Veterinary Science, Mahidol University, Nakornpathom 73710, Thailand.

doi: www.doi.org/10.14202/vetworld.2022.2699-2704

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Article history: Received: 23-08-2022, Accepted: 26-10-2022, Published online: 27-11-2022

Corresponding author: Shutipen Buranasinsup

E-mail: shutipen.bur@mahidol.edu

Citation: Jangsangthong A, Suriyakhun N, Tunyong W, Kong-Ngoen T, Santajit S, Indrawattana N, and Buranasinsup S (2022) Occurrence of antimicrobial resistance and antimicrobial resistance genes in methicillin-resistant Staphylococcus aureus isolated from healthy rabbits, Veterinary World, 15(11): 2699–2704.

Abstract

Background and Aim: Methicillin-resistant globally, Staphylococcus aureus (MRSA) is a major cause of disease in both humans and animals. Several studies have documented the presence of MRSA in healthy and infected animals. However, there is less information on MRSA occurrence in exotic pets, especially healthy rabbits. This study aimed to look into the antimicrobial resistance profile, hidden antimicrobial-resistant genes in isolated bacteria, and to estimate prevalence of MRSA in healthy rabbits.

Materials and Methods: Two-hundreds and eighteen samples, including 42 eyes, 44 ears, 44 oral, 44 ventral thoracic, and 44 perineal swabs, were taken from 44 healthy rabbits that visited the Prasu-Arthorn Animal Hospital, in Nakornpathom, Thailand, from January 2015 to October-2015. The traditional methods of Gram stain, mannitol fermentation, hemolysis on blood agar, catalase test, and coagulase production were used to confirm the presence of Staphylococcus aureus in all specimens. All bacterial isolates were determined by antimicrobial susceptibility test by the disk diffusion method. The polymerase chain reaction was used to identify the antimicrobial-resistant genes (blaZ, mecA, aacA-aphD, msrA, tetK, gyrA, grlA, and dfrG) in isolates of MRSA with a cefoxitin-resistant phenotype.

Results: From 218 specimens, 185 S. aureus were isolated, with the majority of these being found in the oral cavity (29.73%) and ventral thoracic area (22.7%), respectively. Forty-seven (25.41%) MRSAs were found in S. aureus isolates, with the majority of these being found in the perineum (16, 34.04%) and ventral thoracic area (13, 27.66%) specimens. Among MRSAs, 29 (61.7%) isolates were multidrug-resistant (MDR) strains. Most of MRSA isolates were resistant to penicillin (100%), followed by ceftriaxone (44.68%) and azithromycin (44.68%). In addition, these bacteria contained the most drug-resistance genes, blaZ (47.83%), followed by gyrA (36.17%) and tetK (23.4%).

Conclusion: This study revealed that MRSA could be found even in healthy rabbits. Some MRSAs strains were MDR–MRSA, which means that when an infection occurs, the available antibiotics were not effective in treating it. To prevent the spread of MDR–MRSA from pets to owners, it may be helpful to educate owners about effective prevention and hygiene measures.

Keywords: antimicrobial resistance, antimicrobial-resistant genes, healthy rabbits, methicillin-resistant Staphylococcus aureus.